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AB250821

Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EFCAB4A antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

EFCAB4A, CRACR2B, EF-hand calcium-binding domain-containing protein 4A, Calcium release-activated calcium channel regulator 2B, Calcium release-activated channel regulator 2B, CRAC channel regulator 2B

1 Images
Western blot - Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (AB250821)
  • WB

Supplier Data

Western blot - Anti-EFCAB4A antibody [EPR13742] - BSA and Azide free (AB250821)

This data was developed using ab185223, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EFCAB4A antibody [EPR13742] (<a href='/en-us/products/primary-antibodies/efcab4a-antibody-epr13742-ab185223'>ab185223</a>) at 1/1000 dilution

Lane 1:

Human pancreas lysate at 20 µg

Lane 2:

LnCaP cell lysate at 20 µg

Lane 3:

SW480 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13742

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250821 is the carrier-free version of ab185223.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EFCAB4A also known as EF Hand Calcium Binding Domain 4A or STIM2.1 has a significant role in calcium signaling. It is a protein with a molecular mass of approximately 90 kDa. It is mainly expressed in the brain particularly in the cerebral cortex and hippocampus. EFCAB4A interacts directly with calcium ions and contributes to calcium homeostasis within its cellular environment. The presence of EF-hand motifs in its structure suggests a role in calcium binding necessary for its function in calcium signaling pathways.
Biological function summary

EFCAB4A participates in modulating calcium influx as a part of the store-operated calcium entry (SOCE) mechanism. It forms a complex with STIM1 another calcium sensor and helps regulate the function of ORAI1 channels on the plasma membrane. This complex is significant for the refilling of calcium stores in the endoplasmic reticulum. EFCAB4A's activity influences neuronal transmission and plasticity pointing to its involvement in maintaining normal cognitive functions.

Pathways

EFCAB4A is integrated into the calcium signaling and synaptic plasticity pathways. Its interaction with STIM1 and ORAI1 positions it as an important regulator of the cellular calcium influx necessary for signal transduction processes. These pathways connect EFCAB4A to proteins like CAMKII and calcineurin which are involved in various cellular responses and adaptations to changing internal and external signals. The regulation of calcium levels by EFCAB4A supports critical functions like muscle contraction and neurotransmitter release.

Variations in the function of EFCAB4A have been linked to neurodegenerative disorders and cognitive impairments. Dysfunction in EFCAB4A's role in calcium homeostasis may contribute to Alzheimer's disease where calcium dysregulation is a known factor. Additionally evidence associates EFCAB4A dysfunction with epilepsy where abnormal calcium signaling can lead to neuronal hyperactivity. The relationship of EFCAB4A with STIM1 and ORAI1 further connects these disorders as disrupted SOCE can exacerbate neurodegenerative and seizure-related symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in store-operated Ca(2+) entry (SOCE).
See full target information CRACR2B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com