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AB229979

Anti-EFCAB7 antibody

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(1 Publication)

Rabbit Polyclonal EFCAB7 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EFCAB7 aa 1-200.

View Alternative Names

KIAA1799, EFCAB7, EF-hand calcium-binding domain-containing protein 7

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFCAB7 antibody (AB229979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFCAB7 antibody (AB229979)

Paraffin-embedded human kidney tissue stained for EFCAB7 using ab229979 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFCAB7 antibody (AB229979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFCAB7 antibody (AB229979)

Paraffin-embedded human liver cancer tissue stained for EFCAB7 using ab229979 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-EFCAB7 antibody (AB229979)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EFCAB7 antibody (AB229979)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for EFCAB7 (green) using ab229979 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-EFCAB7 antibody (AB229979)
  • WB

Supplier Data

Western blot - Anti-EFCAB7 antibody (AB229979)

All lanes:

Western blot - Anti-EFCAB7 antibody (ab229979) at 1/2000 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

Mouse skeletal muscle tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 72 kDa

Observed band size: 72 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EFCAB7 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

A8K855

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EFCAB7 also known as EF-hand calcium-binding domain 7 is a protein that interacts primarily with molecular processes within cells. It possesses a mass of approximately 55 kDa. Researchers find EFCAB7 expressed mainly in human tissues with particular emphasis in the brain and testes. The protein shows involvement in calcium binding due to the presence of EF-hand motifs which plays a role in modulating its function.
Biological function summary

EFCAB7 contributes to essential cellular functions beyond just calcium binding. It acts as a part of protein complexes that regulate intracellular signaling pathways. Studies show that EFCAB7 plays a role in cilia formation and function which is important for cell motility and signaling. Its interaction with other proteins highlights its significance in cellular dynamics and homeostasis.

Pathways

Scientists have identified EFCAB7 as a critical participant in the primary cilium biogenesis pathway. This involvement links it with the Sonic Hedgehog signaling pathway an important pathway for embryonic development and cell differentiation. EFCAB7 also interacts with Nek1 a kinase known for its role in ciliogenesis indicating a cooperative functionality between these proteins within these pathways.

Alterations or dysregulation in EFCAB7 can impact conditions such as Joubert syndrome a ciliopathy affecting brain development. The protein's interaction with Nek1 also connects it to polycystic kidney disease where ciliary dysfunction is a primary factor. Researchers continue to examine these associations aiming to understand better the pathogenic mechanisms wherein EFCAB7 plays a role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling. Required for the localization of the EVC2 : EVC subcomplex at the base of primary cilia.
See full target information EFCAB7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed41059571

2025

AI662270/GRP94 axis couples the unfolded protein response to mitochondrial dynamics during acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Suling Ding,Wen Liu,Zhiwei Zhang,Xiyang Yang,Dili Sun,Jianfu Zhu,Xiaowei Zhu,Shijun Wang,Mengshi Xie,Hongyu Shi,Junbo Ge,Xiangdong Yang
View all publications
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Recombinant Human EFCAB7 protein

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Product promise

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