AB106429

Anti-EFEMP1/Fibulin-3 antibody

Name: Anti-EFEMP1/Fibulin-3 antibody (ab106429) | Abcam
Brand: Abcam Limited
SKU: AB106429
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal EFEMP1/Fibulin-3 antibody. Suitable for ICC, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EFEMP1.

View Alternative Names

FBLN3, FBNL, EFEMP1, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3

3 Images

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Immunocytochemistry - Anti-EFEMP1/Fibulin-3 antibody (AB106429)

Immunohistochemical analysis of HeLa cells labelling EFEMP1/Fibulin-3 with ab106429 at 20 μg/ml.

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Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB106429)

Lane 1:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab106429) at 0.5 µg/mL

Lane 2:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab106429) at 1 µg/mL

All lanes:

HeLa cell lysate at 15 µg

Predicted band size: 54 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-EFEMP1/Fibulin-3 antibody (AB106429)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EFEMP1/Fibulin-3 antibody (AB106429)

Immunofluorescence analysis of Hela cells labelling Fibulin 3 with ab106429 at 20 ug/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human EFEMP1. The exact immunogen used to generate this antibody is proprietary information.

Q12805

Specificity

ab106429 is predicted not to cross-react with other Fibulin proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "20 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

Antibodies should not be exposed to prolonged high temperatures.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EFEMP1 also known as Fibulin-3 is a member of the fibulin family involved in extracellular matrix (ECM) organization. The protein's mass is approximately 55 kDa. It is expressed in various tissues but researchers find it most common in the eye lung and arterial walls. As a secreted glycoprotein Fibulin-3 plays a vital role in maintaining structural integrity and elasticity in connective tissues.

Biological function summary

EFEMP1/Fibulin-3 participates in ECM modulation and cell signaling. It interacts with other ECM components such as fibronectin and collagen contributing to structural assembly and stability. Although not prominently part of multi-protein complexes Fibulin-3 influences cellular adhesion and migration impacting tissue development and repair mechanisms. Researchers observe its expression during embryogenesis suggesting a role in developmental processes.

Pathways

EFEMP1/Fibulin-3 integrates into matrix-related pathways impacting tissue architecture. It plays a role in the EGF (Epidermal Growth Factor) signaling pathway which is important for various cell survival and proliferation processes. Fibulin-3 exhibits functional relationships with proteins like fibronectin and integrins within these pathways highlighting its importance in mediating cell-matrix interactions and influencing signal transduction cascades.

EFEMP1/Fibulin-3 has notable connections with certain pathologies. Malfunctions in its expression or structure can relate to age-related macular degeneration and various forms of cancer including mesothelioma. In these diseases Fibulin-3 interacts with proteins such as matrix metalloproteinases which degrade extracellular matrix proteins thereby influencing tumor progression and ECM remodeling in affected tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.

See full target information EFEMP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 17:1144421 PubMed36891459

2023

EFEMP1 is a potential biomarker of choroid thickness change in myopia.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Qing Shi,Ting Wan,Bing Li,Tao Li,Xiao-Dong Zhou

BioMed research international 2020:8851480 PubMed33381589

2020

Hypermethylation of EFEMP1 in the Hippocampus May Be Related to the Deficit in Spatial Memory of Rat Neonates Triggered by Repeated Administration of Propofol.

Applications

Unspecified application

Species

Unspecified reactive species

Nu Zhang,Zhiyi Liao,Pinwen Wu,Hao Fang,Guoping Cai

The Journal of investigative dermatology 140:764-773.e4 PubMed31676413

2019

Defining Transcriptional Signatures of Human Hair Follicle Cell States.

Applications

Unspecified application

Species

Unspecified reactive species

Rie Takahashi,Adrienne Grzenda,Thomas F Allison,Jeffrey Rawnsley,Samuel J Balin,Shan Sabri,Kathrin Plath,William E Lowry

Reproduction, fertility, and development : PubMed25989843

2015

Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Li Shao,Ri-Cheng Chian,Yixin Xu,Zhengjie Yan,Yihui Zhang,Chao Gao,Li Gao,Jiayin Liu,Yugui Cui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EFEMP1/Fibulin-3 antibody

Immunocytochemistry - Anti-EFEMP1/Fibulin-3 antibody (AB106429)

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB106429)

Immunocytochemistry/ Immunofluorescence - Anti-EFEMP1/Fibulin-3 antibody (AB106429)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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