AB151976

Anti-EFEMP1/Fibulin-3 antibody

Name: Anti-EFEMP1/Fibulin-3 antibody (ab151976) | Abcam
Brand: Abcam Limited
SKU: AB151976
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal EFEMP1/Fibulin-3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Recombinant fragment samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EFEMP1 aa 150 to C-terminus.

View Alternative Names

FBLN3, FBNL, EFEMP1, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3

5 Images

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Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

10% SDS PAGE

All lanes:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution

Lane 1:

HEK-293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution

Lane 1:

HUVEC whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution

Lane 1:

Mouse lung tissue extracts at 50 µg

Lane 2:

Mouse testis tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Immunohistochemical analysis of paraffin embedded NCIN87 xenograft tissue labeling EFEMP1/Fibulin-3 with ab151976 at a 1/500 dilution.

Supplier Data

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EFEMP1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q12805

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Recombinant fragment": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EFEMP1 also known as Fibulin-3 is a member of the fibulin family involved in extracellular matrix (ECM) organization. The protein's mass is approximately 55 kDa. It is expressed in various tissues but researchers find it most common in the eye lung and arterial walls. As a secreted glycoprotein Fibulin-3 plays a vital role in maintaining structural integrity and elasticity in connective tissues.

Biological function summary

EFEMP1/Fibulin-3 participates in ECM modulation and cell signaling. It interacts with other ECM components such as fibronectin and collagen contributing to structural assembly and stability. Although not prominently part of multi-protein complexes Fibulin-3 influences cellular adhesion and migration impacting tissue development and repair mechanisms. Researchers observe its expression during embryogenesis suggesting a role in developmental processes.

Pathways

EFEMP1/Fibulin-3 integrates into matrix-related pathways impacting tissue architecture. It plays a role in the EGF (Epidermal Growth Factor) signaling pathway which is important for various cell survival and proliferation processes. Fibulin-3 exhibits functional relationships with proteins like fibronectin and integrins within these pathways highlighting its importance in mediating cell-matrix interactions and influencing signal transduction cascades.

EFEMP1/Fibulin-3 has notable connections with certain pathologies. Malfunctions in its expression or structure can relate to age-related macular degeneration and various forms of cancer including mesothelioma. In these diseases Fibulin-3 interacts with proteins such as matrix metalloproteinases which degrade extracellular matrix proteins thereby influencing tumor progression and ECM remodeling in affected tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.

See full target information EFEMP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 14:e1007532 PubMed30102696

2018

A genome-wide association study identifies a susceptibility locus for biliary atresia on 2p16.1 within the gene EFEMP1.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Melissa A Gilbert,Christopher M Grochowski,Deborah McEldrew,Jessica Llewellyn,Orith Waisbourd-Zinman,Hakon Hakonarson,Joan E Bailey-Wilson,Pierre Russo,Rebecca G Wells,Kathleen M Loomes,Nancy B Spinner,Marcella Devoto

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EFEMP1/Fibulin-3 antibody

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Western blot - Anti-EFEMP1/Fibulin-3 antibody (AB151976)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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