Rabbit Polyclonal EFL1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EFL1 aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EFL1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z2Z2
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits.
Alternative names
EFTUD1, FAM42A, EFL1, Elongation factor-like GTPase 1, Elongation factor Tu GTP-binding domain-containing protein 1, Elongation factor-like 1, Protein FAM42A
Recommended products
Rabbit Polyclonal EFL1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EFL1 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EFL1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z2Z2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EFL1 also known as elongation factor-like 1 acts as a GTPase involved in ribosomal subunit maturation. The protein has a molecular mass of approximately 143 kDa. It is expressed in the cytoplasm and nucleolus across various tissues. This protein exhibits activity in remodeling the pre-60S ribosomal subunit assisting in proper ribosome assembly. EFL1's mechanical action includes replacing eIF6 from the pre-60S subunit which enables effective translation initiation.
- Biological function summary
EFL1 facilitates proper formation and function of ribosomes acting as part of the ribosome biogenesis machinery. It partners with SBDS a known ribosome maturation factor to promote GTPase activity which ensures ribosome readiness for translation. EFL1's role remains central to effective synthesis of proteins by supporting efficient building of functional ribosomes a process vital for normal cellular operation.
- Pathways
EFL1 participates in the ribosome biogenesis and translation initiation pathways. Its function links strongly with the SBDS protein coordinating essential functions within the ribosome assembly process. Additionally EFL1 contributes to protein synthesis serving as an important factor to ensure proper ribosome transition into active protein translation cycles. This coordination influences how cells respond and adapt to different metabolic and cellular needs.
- Associated diseases and disorders
EFL1 plays a role in Shwachman-Diamond syndrome alongside the SBDS protein. Mutations affecting EFL1 or its pathway partners can result in impaired ribosome assembly and function contributing to the pathophysiology of this condition. Additionally its involvement in ribosome biogenesis links EFL1 to certain cancer types where disruptions in ribosome function often lead to tumorigenesis. Studies continue to explore these connections highlighting potential therapeutic avenues targeting EFL1’s pathway interactions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFL1 antibody (ab188305)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling EFL1 with ab188305 at 1/20 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFL1 antibody (ab188305)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling EFL1 with ab188305 at 1/20 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFL1 antibody (ab188305)
Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling EFL1 with ab188305 at 1/20 dilution.
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