Anti-EFTUD2 antibody

5 product images

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  Western blot - Anti-EFTUD2 antibody (ab72456)

  All lanes: Western blot - Anti-EFTUD2 antibody (ab72456) at 0.04 µg/mL

  Lane 1: HeLa whole cell lysate at 50 µg

  Lane 2: HeLa whole cell lysate at 15 µg

  Lane 3: HeLa whole cell lysate at 5 µg

  Lane 4: 293T whole cell lysate at 50 µg

  Lane 5: NIH3T3 whole cell lysate at 50 µg

  Predicted band size: 109 kDa

  Observed band size: 117 kDa

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  Immunoprecipitation - Anti-EFTUD2 antibody (ab72456)

  HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab72456 used for IP at 3 µg/mg lysate, and at 0.1 µg/ml for subsequent WB.
  Detection: Chemiluminescence with an exposure time of 10 seconds.

  All lanes: Immunoprecipitation - Anti-EFTUD2 antibody (ab72456)

  Predicted band size: 109 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFTUD2 antibody (ab72456)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling EFTUD2 with ab72456 at 1/200 (1µg/ml). Detection: DAB.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFTUD2 antibody (ab72456)

  IHC image of ab72456 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72456, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

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  Image collected and cropped by CiteAb under a CC-BY license from the publication

  Western blot - Anti-EFTUD2 antibody (ab72456)

  EFTUD2 western blot using anti-EFTUD2 antibody ab72456. Publication image and figure legend from Cloutier, P., Poitras, C., et al., 2017, Nat Commun, PubMed 28561026.

  
  

  ab72456 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab72456 please see the product overview.

  Affinity purification of R2TP/PFDL subunits reveals interactors.(a) Diagram of the network of high-confidence interactions formed around the R2TP/PFD complex. Solid lines denote interactions with a FDR lower than 0.1 while dashed lines are interactions of relevance in this network with FDR scores higher than 0.1, but lower than 0.2 (Supplementary Data 1). Green-coloured nodes are tagged subunits used in this experiment. (b) CoImmunoprecipitation (CoIP) of FLAG-tagged subunits of the R2TP/PFD-like complex (RPAP3 and URI1) in HeLa S3 cells. Various Western blots were made to detect endogenous or recombinant proteins, as marked to the right. (c) Outline of the splicing cycle with emphasis on the recycling steps of the U5 snRNP and its reintegration within the U4/U6.U5 tri-snRNP (inner loop). (d) Regulation of the TORC1 kinase complex by TSC1–TSC2 (annotated here as ‘TSC'). Guanine nucleotides (GTP, GDP) and phosphate groups (P) are indicated in green or red, depending on whether they have an activating or inhibitory effect on their associated proteins, respectively.