Rabbit Polyclonal EFTUD2 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human EFTUD2.
View Alternative Names
KIAA0031, SNRP116, EFTUD2, 116 kDa U5 small nuclear ribonucleoprotein component, Elongation factor Tu GTP-binding domain-containing protein 2, SNU114 homolog, hSNU114, U5-116 kDa
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFTUD2 antibody (AB72456)
IHC image of ab72456 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72456, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFTUD2 antibody (AB72456)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling EFTUD2 with ab72456 at 1/200 (1µg/ml). Detection : DAB.
- IP
Unknown
Immunoprecipitation - Anti-EFTUD2 antibody (AB72456)
HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab72456 used for IP at 3 µg/mg lysate, and at 0.1 µg/ml for subsequent WB.
Detection : Chemiluminescence with an exposure time of 10 seconds.
All lanes:
Immunoprecipitation - Anti-EFTUD2 antibody (ab72456)
Predicted band size: 109 kDa
false
- WB
Unknown
Western blot - Anti-EFTUD2 antibody (AB72456)
All lanes:
Western blot - Anti-EFTUD2 antibody (ab72456) at 0.04 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
HeLa whole cell lysate at 15 µg
Lane 3:
HeLa whole cell lysate at 5 µg
Lane 4:
293T whole cell lysate at 50 µg
Lane 5:
NIH3T3 whole cell lysate at 50 µg
Predicted band size: 109 kDa
Observed band size: 117 kDa
false
- WB
CiteAb
Western blot - Anti-EFTUD2 antibody (AB72456)
EFTUD2 western blot using anti-EFTUD2 antibody ab72456. Publication image and figure legend from Cloutier, P., Poitras, C., et al., 2017, Nat Commun, PubMed 28561026.
ab72456 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab72456 please see the product overview.
Affinity purification of R2TP/PFDL subunits reveals interactors.(a) Diagram of the network of high-confidence interactions formed around the R2TP/PFD complex. Solid lines denote interactions with a FDR lower than 0.1 while dashed lines are interactions of relevance in this network with FDR scores higher than 0.1, but lower than 0.2 (Supplementary Data 1). Green-coloured nodes are tagged subunits used in this experiment. (b) CoImmunoprecipitation (CoIP) of FLAG-tagged subunits of the R2TP/PFD-like complex (RPAP3 and URI1) in HeLa S3 cells. Various Western blots were made to detect endogenous or recombinant proteins, as marked to the right. (c) Outline of the splicing cycle with emphasis on the recycling steps of the U5 snRNP and its reintegration within the U4/U6.U5 tri-snRNP (inner loop). (d) Regulation of the TORC1 kinase complex by TSC1–TSC2 (annotated here as ‘TSC'). Guanine nucleotides (GTP, GDP) and phosphate groups (P) are indicated in green or red, depending on whether they have an activating or inhibitory effect on their associated proteins, respectively.
false
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Reactivity data
Properties and storage information
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Aliquoting information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EFTUD2 participates in RNA processing activities that are necessary for accurate gene expression. This protein interacts with other components in the spliceosomal complex such as PRPF8 and SNRNP200 to facilitate the excision of introns and the ligation of exons. The precision in these activities ensures that transcription processes occur correctly and mutations in EFTUD2 can lead to mis-spliced mRNA and abnormal proteins disrupting normal cellular functions.
Pathways
EFTUD2 is an important participant in the mRNA splicing pathway an integral part of the gene expression pathway. The successful regulation of this splicing pathway is necessary for the proper synthesis of most eukaryotic proteins. EFTUD2 collaborates with proteins like SF3B1 and SYF2 to carry out efficient splicing ensuring the upkeep of normal cellular physiology and adaptive responses to environmental stimuli.
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Target data
Publications (8)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in oncology 15:1499217 PubMed40236649
2025
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Disease models & mechanisms 17: PubMed38426258
2024
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Neural regeneration research 18:856-862 PubMed36204854
2022
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Journal of cancer research and clinical oncology 149:4293-4303 PubMed36068443
2022
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Nucleic acids research 50:1128-1146 PubMed34951455
2021
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Nature communications 12:4810 PubMed34376666
2021
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Nature communications 11:2412 PubMed32415063
2020
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Nature communications 8:15615 PubMed28561026
2017
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WB
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Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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