AB150375

Anti-EG-VEGF antibody [EPR9699]

RabMAb
Recombinant
What is this?

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(14 Publications)

Rabbit Recombinant Monoclonal EG-VEGF antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 14 publications.

View Alternative Names

UNQ600/PRO1186, PROK1, Prokineticin-1, Endocrine-gland-derived vascular endothelial growth factor, Mambakine, EG-VEGF

2 Images

Flow Cytometry (Intracellular) - Anti-EG-VEGF antibody [EPR9699] (AB150375)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-EG-VEGF antibody [EPR9699] (AB150375)

Intracellular flow cytometric analysis of permeabilized NIH/3T3 cells labelling EG-VEGF with ab150375 at 1/10 dilution (red) or a Rabbit IgG (negative, green).

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Western blot - Anti-EG-VEGF antibody [EPR9699] (AB150375)

All lanes:

Western blot - Anti-EG-VEGF antibody [EPR9699] (ab150375) at 1/1000 dilution

Lane 1:

Raw 264.7 cell lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate at 10 µg

Lane 3:

ECV-304 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 12 kDa

false

Carrier free

Anti-EG-VEGF antibody [EPR9699] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9699

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EG-VEGF also known as endocrine gland-derived vascular endothelial growth factor or prokineticin 1 is a potent angiogenic mitogen. It typically has a molecular mass of around 9 kDa. EG-VEGF is expressed mainly in steroidogenic tissues such as the ovaries testes adrenal glands and placenta. It targets endothelial cells selectively within these glands inducing various responses like proliferation migration and fenestration. This selectivity makes it distinct from vascular endothelial growth factor A (VEGF-A) which has a broader expression pattern.

Biological function summary

EG-VEGF plays a critical role in the formation and remodeling of blood vessels specific to endocrine glands. It is not part of a larger protein complex but operates through interaction with its receptor prokineticin receptor 1 (PROKR1). This unique interaction means that it has a specific ligand-receptor activity contributing to the regulation of vascular development especially in tissues undergoing hormonal changes. This makes EG-VEGF significant in contexts where localized and specific angiogenesis is essential.

Pathways

EG-VEGF is important in the regulation of angiogenesis and endothelial function. It fits into pathways that influence cell signaling associated with vascular development. One such pathway is the hypoxia-inducible factor (HIF) pathway which enhances the expression of EG-VEGF under low oxygen conditions. Another related protein is vascular endothelial growth factor (VEGF) whereby VEGF and EG-VEGF demonstrate distinctive yet occasionally overlapping functions in angiogenesis optimizing vascular growth within specific environments.

EG-VEGF shows associations with conditions like preeclampsia and polycystic ovary syndrome (PCOS). Preeclampsia involves improper vascularization in the placenta where EG-VEGF's role in angiogenesis becomes critical as it may help or disrupt proper vascular development. Polycystic ovary syndrome a hormonal disorder impacting ovaries also links with EG-VEGF through its effect on ovarian blood vessels. These disorders highlight potential therapeutic targets considering EG-VEGF’s integration with other proteins like PROKR1 which could modulate its angiogenic functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.

See full target information PROK1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40806274

2025

Hormonal Signaling and Follicular Regulation in Normal and Miniature Pigs During Corpus Luteum Regression.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Hwan Kim

Stem cell research & therapy 16:253 PubMed40394699

2025

Exosomes derived from adipose-derived stem cells alleviate acute radiation-induced dermatitis through up-regulating hyaluronic acid synthase 1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Meijia Li,Yuan Tian,Xiaotian Wang,Di Sun,Haiqian Xu,Xinyue Wang,Xinyue Chen,Lijun Hao

Heliyon 10:e38599 PubMed39435107

2024

Promoting diabetic oral mucosa wound healing with a light-responsive hydrogel adaptive to the microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwen Ding,Xiaohui Zhang,Gaopeng Wang,Jiaying Shi,Jiayu Zhu,Jiayu Yan,Jing Wang,Junhua Wu

Clinical and experimental pharmacology & physiology 51:e13855 PubMed38636942

2024

Salvianic acid A sodium facilitates cardiac microvascular endothelial cell proliferation by enhancing the hypoxia-inducible factor-1 alpha/vascular endothelial growth factor signalling pathway post-myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Jichun Liu,Fei Wu,Zhenhan Li,Shengwei Zheng,Yanqiang Huang,Hao Chen

Tissue barriers 13:2334544 PubMed38544287

2024

Fibroblasts/three-dimensional scaffolds complexes promote wound healing in rats with skin defects.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Jiang,Qiang Liu,Er-Chang Xu,Si-Yu He,Hong-Yan Liu,Chao Tian,Lan-Fang Zhang,Ze-Long Yang

Heliyon 10:e27653 PubMed38524558

2024

LncRNA NEAT1 promotes angiogenesis of retinoblastoma cells through regulation of the miR-106a/HIF-1α axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Zhiyuan Xin,Kun Zhang,Xin Jin,Dajiang Wang

Integrative cancer therapies 22:15347354231172732 PubMed37157810

2023

6-Shogaol Inhibits the Cell Migration of Colon Cancer by Suppressing the EMT Process Through the IKKβ/NF-κB/Snail Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Min Chen,Chiin Tong,Qibiao Wu,Zhenghong Zhong,Qida He,Li Zeng,Lu Xiao

Ginekologia polska : PubMed35942721

2022

LncRNA-loc391533 is involved in the progression of preeclampsia through VEGF.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Xiong,Xuan Jin,Kangxiang Xu,Bingqi Wu,Yuqi Xu,Dong Ruan,Xiaoju He

Stem cell research & therapy 13:336 PubMed35870977

2022

Exosomes from mmu_circ_0001052-modified adipose-derived stem cells promote angiogenesis of DFU via miR-106a-5p and FGF4/p38MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zun-Hong Liang,Nan-Fang Pan,Shi-Shuai Lin,Zhi-Yang Qiu,Ping Liang,Jun Wang,Zhi Zhang,Yun-Chuan Pan

Bioengineered 12:7446-7458 PubMed34652260

2021

Long non-coding RNA SNHG22 facilitates hepatocellular carcinoma tumorigenesis and angiogenesis via DNA methylation of microRNA miR-16-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yinxin Zhang,Changliang Lu,Haiwei Cui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EG-VEGF antibody [EPR9699]

Flow Cytometry (Intracellular) - Anti-EG-VEGF antibody [EPR9699] (AB150375)

Western blot - Anti-EG-VEGF antibody [EPR9699] (AB150375)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise