AB61199

Anti-Eg5 antibody

Name: Anti-Eg5 antibody (ab61199) | Abcam
Brand: Abcam Limited
SKU: AB61199
Price: 535 USD
Availability: InStock

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(11 Publications)

Rabbit Polyclonal Eg5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human KIF11.

View Alternative Names

EG5, KNSL1, TRIP5, KIF11, Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody (AB61199)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody (AB61199)

ab61199 at 1/50 dilution staining Eg5 in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.

Unknown

Western blot - Anti-Eg5 antibody (AB61199)

All lanes:

Western blot - Anti-Eg5 antibody (ab61199) at 1/500 dilution

Lane 1:

COLO205 cell extracts

Lane 2:

COLO205 cell extracts with immunising peptide

Predicted band size: 119 kDa

Observed band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human KIF11. The exact immunogen used to generate this antibody is proprietary information.

P52732

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab61199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Eg5 protein also known scientifically as kinesin family member 11 (KIF11) plays an important role in cell mitosis. It functions mechanically as a plus-end directed motor involved in the separation of centrosomes during the early stage of cell division. Eg5 with an approximate mass of 116 kDa is responsible for the proper alignment and segregation of spindle fibers. The protein is expressed in rapidly dividing cells particularly within tissues with high turnover rates such as bone marrow and epithelium.

Biological function summary

The motor function of Eg5 is indispensable for the mitotic spindle organization. It provides the force necessary to push microtubules in opposite directions. Eg5 functions as part of a complex cellular machinery that includes proteins like dynein and other kinesins. By controlling spindle bipolarity it ensures accurate chromosome segregation during cell division preventing errors that could lead to aneuploidy.

Pathways

The action of Eg5 is fundamental in mitotic pathways including the spindle assembly checkpoint pathway. It interacts with proteins such as cyclin-dependent kinases (CDKs) which regulate its activity to ensure proper cell cycle progression. The protein's role is integral in facilitating the dynamics of the metaphase spindle checkpoint guaranteeing cell cycle fidelity.

Eg5 is linked to certain types of cancers where there is abnormal cell division. The overexpression or dysregulation of Eg5 can contribute to tumorigenesis. Additionally Eg5 interacts with proteins involved in cancer such as p53 a tumor suppressor protein directly influencing cellular proliferation and survival. Researchers target Eg5 in the development of anticancer therapies to inhibit its activity aiming to obstruct malignant cell proliferation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis (PubMed : 19001501, PubMed : 37728657). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed : 23857769).

See full target information KIF11

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 21:205 PubMed33574944

2021

Screening and identification of hub genes in bladder cancer by bioinformatics analysis and KIF11 is a potential prognostic biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Cong Mo,Zi-Tong Zhang,Meng-Jia Song,Zi-Qi Zhou,Jian-Xiong Zeng,Yu-Fei Du,Feng-Ze Sun,Jie-Ying Yang,Jun-Yi He,Yue Huang,Jian-Chuan Xia,De-Sheng Weng

BioMed research international 2021:2676745 PubMed33490265

2021

KIF11 Promotes Proliferation of Hepatocellular Carcinoma among Patients with Liver Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan-Dong Hu,Ying Jiang,Hong-Mei Sun,Jing-Wen Wang,Li-Li Zhai,Zhi-Qi Yin,Jun Yan

eLife 9: PubMed33174839

2020

Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Serra-Marques,Maud Martin,Eugene A Katrukha,Ilya Grigoriev,Cathelijn Ae Peeters,Qingyang Liu,Peter Jan Hooikaas,Yao Yao,Veronika Solianova,Ihor Smal,Lotte B Pedersen,Erik Meijering,Lukas C Kapitein,Anna Akhmanova

Journal of clinical pathology 72:354-362 PubMed30819726

2019

High kinesin family member 11 expression predicts poor prognosis in patients with clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Jin,Yanfeng Dai,Yan Wang,Shu Zhang,Gang Liu

Small (Weinheim an der Bergstrasse, Germany) 14:e1802565 PubMed30230235

2018

Redox-Responsive Nanoparticle-Mediated Systemic RNAi for Effective Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoding Xu,Jun Wu,Shuaishuai Liu,Phei Er Saw,Wei Tao,Yujing Li,Lisa Krygsman,Srinivasan Yegnasubramanian,Angelo M De Marzo,Jinjun Shi,Charles J Bieberich,Omid C Farokhzad

International journal of oncology 53:1752-1762 PubMed30015883

2018

Circular RNA‑MTO1 suppresses breast cancer cell viability and reverses monastrol resistance through regulating the TRAF4/Eg5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxiao Liu,Yanyan Dong,Liping Zhao,Lihong Su,Jin Luo

PloS one 11:e0154531 PubMed27140416

2016

Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq.

Applications

Species

Unspecified reactive species

S Marthandan,M Baumgart,S Priebe,M Groth,J Schaer,C Kaether,R Guthke,A Cellerino,M Platzer,S Diekmann,P Hemmerich

Cell reports 15:1111-1122 PubMed27117404

2016

Fbxo30 Regulates Mammopoiesis by Targeting the Bipolar Mitotic Kinesin Eg5.

Applications

Species

Mouse

Yan Liu,Yin Wang,Zhanwen Du,Xiaoli Yan,Pan Zheng,Yang Liu

BioMed research international 2015:731938 PubMed26339636

2015

Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts.

Applications

Species

Unspecified reactive species

Shiva Marthandan,Steffen Priebe,Mario Baumgart,Marco Groth,Alessandro Cellerino,Reinhard Guthke,Peter Hemmerich,Stephan Diekmann

Cancer research 71:1219-28 PubMed21303978

2011

Tumor suppressor RARRES1 interacts with cytoplasmic carboxypeptidase AGBL2 to regulate the α-tubulin tyrosination cycle.

Applications

Species

Human

Ziad J Sahab,Michael D Hall,You Me Sung,Sivanesan Dakshanamurthy,Yun Ji,Deepak Kumar,Stephen W Byers

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Eg5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody (AB61199)

Western blot - Anti-Eg5 antibody (AB61199)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise