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AB181981

Anti-Eg5 antibody [EPR12280-76]

5

(2 Reviews)

|

(4 Publications)

Rabbit Recombinant Monoclonal Eg5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

EG5, KNSL1, TRIP5, KIF11, Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR12280-76] (AB181981)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR12280-76] (AB181981)

Immunofluorescence analysis of -20°C acetone fixed HeLa cells labeling Eg5 with ab181981 at 1/100 dilution followed by goat anti rabbit IgG (Dylight 555) at a 1/1000 dilution. Counter stained with Dapi.

Western blot - Anti-Eg5 antibody [EPR12280-76] (AB181981)
  • WB

Supplier Data

Western blot - Anti-Eg5 antibody [EPR12280-76] (AB181981)

All lanes:

Western blot - Anti-Eg5 antibody [EPR12280-76] (ab181981) at 1/10000 dilution

Lane 1:

Raji cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 119 kDa

false

  • Carrier free

    Anti-Eg5 antibody [EPR12280-76] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12280-76

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Eg5 protein also known scientifically as kinesin family member 11 (KIF11) plays an important role in cell mitosis. It functions mechanically as a plus-end directed motor involved in the separation of centrosomes during the early stage of cell division. Eg5 with an approximate mass of 116 kDa is responsible for the proper alignment and segregation of spindle fibers. The protein is expressed in rapidly dividing cells particularly within tissues with high turnover rates such as bone marrow and epithelium.
Biological function summary

The motor function of Eg5 is indispensable for the mitotic spindle organization. It provides the force necessary to push microtubules in opposite directions. Eg5 functions as part of a complex cellular machinery that includes proteins like dynein and other kinesins. By controlling spindle bipolarity it ensures accurate chromosome segregation during cell division preventing errors that could lead to aneuploidy.

Pathways

The action of Eg5 is fundamental in mitotic pathways including the spindle assembly checkpoint pathway. It interacts with proteins such as cyclin-dependent kinases (CDKs) which regulate its activity to ensure proper cell cycle progression. The protein's role is integral in facilitating the dynamics of the metaphase spindle checkpoint guaranteeing cell cycle fidelity.

Eg5 is linked to certain types of cancers where there is abnormal cell division. The overexpression or dysregulation of Eg5 can contribute to tumorigenesis. Additionally Eg5 interacts with proteins involved in cancer such as p53 a tumor suppressor protein directly influencing cellular proliferation and survival. Researchers target Eg5 in the development of anticancer therapies to inhibit its activity aiming to obstruct malignant cell proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis (PubMed : 19001501, PubMed : 37728657). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed : 23857769).
See full target information KIF11
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

World journal of surgical oncology 18:56 PubMed32183822

2020

Circ-FOXM1 contributes to cell proliferation, invasion, and glycolysis and represses apoptosis in melanoma by regulating miR-143-3p/FLOT2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Tian,Gangwen Han,Lulu Lu,Xiangyu Meng

International journal of biological sciences 16:1388-1402 PubMed32210727

2020

The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xu,Minghua Huang,Jia Guo,Huiting Zhang,Depeng Wang,Tiantian Liu,Haiting Liu,Shiming Chen,Peng Gao,Kun Mu

Molecular biology of the cell 30:42-55 PubMed30379607

2018

Environmental stresses induce karyotypic instability in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihao Tan,Yong Jie Andrew Chan,Ying Jie Karen Chua,Samuel D Rutledge,Norman Pavelka,Daniela Cimini,Giulia Rancati

Molecular biology of the cell 29:2370-2377 PubMed30091644

2018

CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Kuan-Chung Su,Mary-Jane Tsang,Neil Emans,Iain M Cheeseman
View all publications
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Product promise

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