JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272226

Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Eg5 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

View Alternative Names

EG5, KNSL1, TRIP5, KIF11, Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Eg5 with ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in spermatocytes and spermatogonnia of rat testis is observed. The section was incubated with ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Eg5 with ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Scattered cytoplasmic staining in human breast cancer cells is observed (PMID : 29181100). The section was incubated with ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)

Intracellular flow cytometric analysis of 80% methanol fixed 0.1% Tween-20 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Eg5 with ab272220 at 1/50 dilution (0.1μg)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were pre-treated with 20μg/ml RNaseA for 30min to minimize the binding between Propidium Iodide (PI) and RNA. Then intracellularly stained with ab272220 and PI. Propidium Iodide Flow Cytometry Kit (ab139418) was used for RNaseA treatment and PI staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Eg5 with ab272220 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing spindle fibre and cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Eg5 with ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in spermatocytes and spermatogonnia of mouse testis is observed. The section was incubated with ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Eg5 with ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in germinal center of human tonsil is observed (PMID : 25277178). The section was incubated with ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling Eg5 with ab272220 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing spindle fibre and cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272220).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23277-7

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

ab272226 is the carrier-free version of ab272220.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Eg5 protein also known scientifically as kinesin family member 11 (KIF11) plays an important role in cell mitosis. It functions mechanically as a plus-end directed motor involved in the separation of centrosomes during the early stage of cell division. Eg5 with an approximate mass of 116 kDa is responsible for the proper alignment and segregation of spindle fibers. The protein is expressed in rapidly dividing cells particularly within tissues with high turnover rates such as bone marrow and epithelium.
Biological function summary

The motor function of Eg5 is indispensable for the mitotic spindle organization. It provides the force necessary to push microtubules in opposite directions. Eg5 functions as part of a complex cellular machinery that includes proteins like dynein and other kinesins. By controlling spindle bipolarity it ensures accurate chromosome segregation during cell division preventing errors that could lead to aneuploidy.

Pathways

The action of Eg5 is fundamental in mitotic pathways including the spindle assembly checkpoint pathway. It interacts with proteins such as cyclin-dependent kinases (CDKs) which regulate its activity to ensure proper cell cycle progression. The protein's role is integral in facilitating the dynamics of the metaphase spindle checkpoint guaranteeing cell cycle fidelity.

Eg5 is linked to certain types of cancers where there is abnormal cell division. The overexpression or dysregulation of Eg5 can contribute to tumorigenesis. Additionally Eg5 interacts with proteins involved in cancer such as p53 a tumor suppressor protein directly influencing cellular proliferation and survival. Researchers target Eg5 in the development of anticancer therapies to inhibit its activity aiming to obstruct malignant cell proliferation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis (PubMed : 19001501, PubMed : 37728657). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed : 23857769).
See full target information KIF11
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB254298

Anti-Eg5 antibody [EPR23276-52]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23276-52] (AB254298)

  • 0
  • 0
Primary Antibodies

AB249676

Anti-Eg5 antibody [EPR12280] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Eg5 antibody [EPR12280] - BSA and Azide free (AB249676)

  • 0
  • 0
Primary Antibodies

AB254299

Anti-Eg5 antibody [EPR23277-60]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-60] (AB254299)

  • 0
  • 0
Primary Antibodies

AB270754

Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23276-52] - BSA and Azide free (AB270754)

  • 0
  • 0
Primary Antibodies

AB270409

Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (AB270409)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB31645

Anti-hnRNP A2B1 antibody

BOND RX™ Validated

Immunocytochemistry - Anti-hnRNP A2B1 antibody (AB31645)

  • 5
  • 5
Primary Antibodies

AB283500

Anti-Cofilin (phospho S3) antibody [EPR24753-28]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin (phospho S3) antibody [EPR24753-28] (AB283500)

  • 0
  • 0
Primary Antibodies

AB32064

Anti-Cleaved PARP1 antibody [E51]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (AB32064)

  • 4
  • 18
Primary Antibodies

AB129113

Anti-FKBP38 antibody [EPR7441(2)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-FKBP38 antibody [EPR7441(2)] (AB129113)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com