Rabbit Recombinant Monoclonal Eg5 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62622.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)")
![Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunocytochemistry-immunofluorescence-img76563.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)")
![Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--flow-cytometry-intracellular-img17640.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58119.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)")
![Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunocytochemistry-immunofluorescence-img23935.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (AB272226)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-Fr | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Tested | Not recommended | Tested | Expected |
| Rat | Not recommended | Tested | Expected | Not recommended | Tested | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis (PubMed:19001501, PubMed:37728657). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).
Alternative names
EG5, KNSL1, TRIP5, KIF11, Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5
Recommended products
Rabbit Recombinant Monoclonal Eg5 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23277-7
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab272226 is the carrier-free version of Anti-Eg5 antibody [EPR23277-7] ab272220.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Eg5 protein also known scientifically as kinesin family member 11 (KIF11) plays an important role in cell mitosis. It functions mechanically as a plus-end directed motor involved in the separation of centrosomes during the early stage of cell division. Eg5 with an approximate mass of 116 kDa is responsible for the proper alignment and segregation of spindle fibers. The protein is expressed in rapidly dividing cells particularly within tissues with high turnover rates such as bone marrow and epithelium.
- Biological function summary
The motor function of Eg5 is indispensable for the mitotic spindle organization. It provides the force necessary to push microtubules in opposite directions. Eg5 functions as part of a complex cellular machinery that includes proteins like dynein and other kinesins. By controlling spindle bipolarity it ensures accurate chromosome segregation during cell division preventing errors that could lead to aneuploidy.
- Pathways
The action of Eg5 is fundamental in mitotic pathways including the spindle assembly checkpoint pathway. It interacts with proteins such as cyclin-dependent kinases (CDKs) which regulate its activity to ensure proper cell cycle progression. The protein's role is integral in facilitating the dynamics of the metaphase spindle checkpoint guaranteeing cell cycle fidelity.
- Associated diseases and disorders
Eg5 is linked to certain types of cancers where there is abnormal cell division. The overexpression or dysregulation of Eg5 can contribute to tumorigenesis. Additionally Eg5 interacts with proteins involved in cancer such as p53 a tumor suppressor protein directly influencing cellular proliferation and survival. Researchers target Eg5 in the development of anticancer therapies to inhibit its activity aiming to obstruct malignant cell proliferation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img62622.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Eg5 with Anti-Eg5 antibody [EPR23277-7] ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in germinal center of human tonsil is observed (PMID:25277178). The section was incubated with Anti-Eg5 antibody [EPR23277-7] ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Eg5 antibody [EPR23277-7] ab272220).
![Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunocytochemistry-immunofluorescence-img76563.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)")
Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Eg5 with Anti-Eg5 antibody [EPR23277-7] ab272220 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing spindle fibre and cytoplasmic staining in HeLa cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Eg5 antibody [EPR23277-7] ab272220).
![Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--flow-cytometry-intracellular-img17640.jpg "Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)")
Flow Cytometry (Intracellular) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
Intracellular flow cytometric analysis of 80% methanol fixed 0.1% Tween-20 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Eg5 with Anti-Eg5 antibody [EPR23277-7] ab272220 at 1/50 dilution (0.1μg)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were pre-treated with 20μg/ml RNaseA for 30min to minimize the binding between Propidium Iodide (PI) and RNA. Then intracellularly stained with Anti-Eg5 antibody [EPR23277-7] ab272220 and PI. Propidium Iodide Flow Cytometry Kit (Propidium Iodide Flow Cytometry Kit ab139418) was used for RNaseA treatment and PI staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Eg5 antibody [EPR23277-7] ab272220).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58119.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Eg5 with Anti-Eg5 antibody [EPR23277-7] ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in spermatocytes and spermatogonnia of mouse testis is observed. The section was incubated with Anti-Eg5 antibody [EPR23277-7] ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Eg5 antibody [EPR23277-7] ab272220).
![Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunocytochemistry-immunofluorescence-img23935.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)")
Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling Eg5 with Anti-Eg5 antibody [EPR23277-7] ab272220 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing spindle fibre and cytoplasmic staining in NIH/3T3 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Eg5 antibody [EPR23277-7] ab272220).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92604.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Eg5 with Anti-Eg5 antibody [EPR23277-7] ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in spermatocytes and spermatogonnia of rat testis is observed. The section was incubated with Anti-Eg5 antibody [EPR23277-7] ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Eg5 antibody [EPR23277-7] ab272220).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77406.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Eg5 with Anti-Eg5 antibody [EPR23277-7] ab272220 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Scattered cytoplasmic staining in human breast cancer cells is observed (PMID:29181100). The section was incubated with Anti-Eg5 antibody [EPR23277-7] ab272220 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Eg5 antibody [EPR23277-7] ab272220).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92604.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226), expandable thumbnail](https://content.abcam.com/products/images/eg5-antibody-epr23277-7-bsa-and-azide-free-ab272226--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77406.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-7] - BSA and Azide free (ab272226)")
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