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AB9695

Anti-EGF antibody

5

(3 Reviews)

|

(36 Publications)

Anti-EGF antibody (ab9695) is a rabbit polyclonal antibody detecting EGF in Western Blot, IHC-P, ELISA, Neut. Suitable for Human,.

- Over 30 publications
- Trusted since 2002

View Alternative Names

Pro-epidermal growth factor, EGF

2 Images
Western blot - Anti-EGF antibody (AB9695)
  • WB

Unknown

Western blot - Anti-EGF antibody (AB9695)

Primary ab9695 was used at a dilution of 0.2 ug/ml.

Lane 1 : 250 ng Recombinant human EGF protein

Lane 2 : 125 ng Recombinant human EGF protein

All lanes:

Western blot - Anti-EGF antibody (ab9695) at 0.2 µg/mL

Lane 1:

Recombinant Human EGF protein (<a href='/en-us/products/unavailable/recombinant-human-egf-protein-ab55483'>ab55483</a>) at 0.1 µg

Lane 2:

Recombinant Human EGF protein (<a href='/en-us/products/unavailable/recombinant-human-egf-protein-ab55483'>ab55483</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 133 kDa

true

Exposure time: 1min

Sandwich ELISA - Anti-EGF antibody (AB9695)
  • sELISA

Unknown

Sandwich ELISA - Anti-EGF antibody (AB9695)

Standard Curve for EGF (Analyte : ab51082) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [S-177] to EGF (ab18635) at 5ug/ml and Detector Antibody Rabbit polyclonal to EGF (ab9695) at 0.5ug/ml

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

ELISA, sELISA, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EGF.

P01133

Reactivity data

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Product details

What is this antibody validated in?
Anti-EGF antibody (ab9695) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), ELISA, Neutralising in Human, samples.

Trusted by the scientific community
Anti-EGF (ab9695) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.

Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute with 200µl of sterile water.
Purification technique
Affinity purification Immunogen
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.
Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed : 10964941).
See full target information EGF

Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Acta cirurgica brasileira 39:e391224 PubMed38477786

2024

Ellagic acid protected the gingival tissue via fibroblast and epidermal growth factors in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Gülüçağ Giray Tekin,Buşra Deveci,Engin Deveci

International journal of molecular sciences 24: PubMed38003736

2023

Distinct HAND2/HAND2-AS1 Expression Levels May Fine-Tune Mesenchymal and Epithelial Cell Plasticity of Human Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Vazana-Netzarim,Yishay Elmalem,Shachar Sofer,Hod Bruck,Naama Danino,Udi Sarig

Acta cirurgica brasileira 38:e381723 PubMed37098927

2023

Investigation of the histopathological level of Ki-67, caspase-3 expressions of the effects of hesperidin on wound healing in the rat esophagus.

Applications

Unspecified application

Species

Unspecified reactive species

Cemalettin Durgun,Gülsen Kirman,Engin Deveci

Scientific reports 13:5144 PubMed36991029

2023

p130Cas is required for androgen-dependent postnatal development regulation of submandibular glands.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Gao,Aonan Li,Shinsuke Fujii,Fei Huang,Chihiro Nakatomi,Ichiro Nakamura,Hiroaki Honda,Tamotsu Kiyoshima,Eijiro Jimi

Annals of the New York Academy of Sciences 1518:299-314 PubMed36303271

2022

Epidermal growth factor activates a hypoxia-inducible factor 1α-microRNA-21 axis to inhibit aquaporin 4 in chronic rhinosinusitis.

Applications

Unspecified application

Species

Unspecified reactive species

Junjun Chen,Dong Liu,Jingpu Yang,Chengxun Jin,Chang Zhao,Jinzhang Cheng

International journal of medical sciences 19:1779-1786 PubMed36313223

2022

The Potential of Transforming Growth Factor-beta Inhibitor and Vascular Endothelial Growth Factor Inhibitor as Therapeutic Agents for Uterine Leiomyoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Yoon Park,Boah Chae,Mee-Ran Kim

Journal of applied oral science : revista FOB 30:e20210413 PubMed35195153

2022

Expression of epithelial growth factors and of apoptosis-regulating proteins, and presence of CD57+ cells in the development of inflammatory periapical lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Walter Arthur Silva Valente,Déborah Barrocas,Luciana Armada,Fábio Ramôa Pires

British journal of cancer 125:390-401 PubMed34088989

2021

The role of anlotinib-mediated EGFR blockade in a positive feedback loop of CXCL11-EGF-EGFR signalling in anaplastic thyroid cancer angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Juyong Liang,Zhijian Jin,Jie Kuang,Haoran Feng,Qiwu Zhao,Zheyu Yang,Ling Zhan,Baiyong Shen,Jiqi Yan,Wei Cai,Xi Cheng,Weihua Qiu

International journal of molecular sciences 22: PubMed33800168

2021

Gene Knockout Contributes to Human Epidermal Growth Factor Production in Transgenic Silkworms.

Applications

Unspecified application

Species

Unspecified reactive species

Meiyu Wu,Jinghua Ruan,Xiaogang Ye,Shuo Zhao,Xiaoli Tang,Xiaoxiao Wang,Huiping Li,Boxiong Zhong

Stem cells (Dayton, Ohio) 39:581-599 PubMed33547700

2021

SMAD-specific E3 ubiquitin ligase 2 promotes angiogenesis by facilitating PTX3 degradation in MSCs from patients with ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjun Ma,Wen Yang,Zhaopeng Cai,Peng Wang,Hongyu Li,Rujia Mi,Yuhang Jiang,Zhongyu Xie,Pengfei Sui,Yanfeng Wu,Huiyong Shen
View all publications

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