Anti-EGF antibody
5
(3 Reviews)
|
(36 Publications)
Anti-EGF antibody (ab9695) is a rabbit polyclonal antibody detecting EGF in Western Blot, IHC-P, ELISA, Neut. Suitable for Human,.
- Over 30 publications
- Trusted since 2002
View Alternative Names
Pro-epidermal growth factor, EGF
- WB
Unknown
Western blot - Anti-EGF antibody (AB9695)
Primary ab9695 was used at a dilution of 0.2 ug/ml.
Lane 1 : 250 ng Recombinant human EGF protein
Lane 2 : 125 ng Recombinant human EGF protein
All lanes:
Western blot - Anti-EGF antibody (ab9695) at 0.2 µg/mL
Lane 1:
Recombinant Human EGF protein (<a href='/en-us/products/unavailable/recombinant-human-egf-protein-ab55483'>ab55483</a>) at 0.1 µg
Lane 2:
Recombinant Human EGF protein (<a href='/en-us/products/unavailable/recombinant-human-egf-protein-ab55483'>ab55483</a>) at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
Predicted band size: 133 kDa
true
Exposure time: 1min
Reactivity data
Product details
Anti-EGF antibody (ab9695) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), ELISA, Neutralising in Human, samples.
Trusted by the scientific community
Anti-EGF (ab9695) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.
Properties and storage information
Form
Reconstitution
Purification technique
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.
Pathways
EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.
Product protocols
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Target data
Publications (36)
Recent publications for all applications. Explore the full list and refine your search
Acta cirurgica brasileira 39:e391224 PubMed38477786
2024
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International journal of molecular sciences 24: PubMed38003736
2023
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Acta cirurgica brasileira 38:e381723 PubMed37098927
2023
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Scientific reports 13:5144 PubMed36991029
2023
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Annals of the New York Academy of Sciences 1518:299-314 PubMed36303271
2022
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International journal of medical sciences 19:1779-1786 PubMed36313223
2022
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Journal of applied oral science : revista FOB 30:e20210413 PubMed35195153
2022
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British journal of cancer 125:390-401 PubMed34088989
2021
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International journal of molecular sciences 22: PubMed33800168
2021
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Stem cells (Dayton, Ohio) 39:581-599 PubMed33547700
2021
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com