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Rabbit Polyclonal EGF antibody. Suitable for ELISA, WB, sELISA, IHC-P, Neut and reacts with Recombinant full length protein, Recombinant full length protein - Human, Human, Recombinant fragment samples. Cited in 34 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EGF.
Alternative names=Pro-epidermal growth factor, EGF, EGF
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
Recombinant Full Length Protein corresponding to Human EGF. Database link: P01133
Consider this alternative
Reactivity data
Select an application| ELISA | WB | sELISA | IHC-P | Neut | |
|---|---|---|---|---|---|
| Human | Predicted | Predicted | Predicted | Expected | Expected |
| Recombinant fragment | Not recommended | Not recommended | Not recommended | Not recommended | Expected |
| Recombinant full length protein | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Tested | Tested | Not recommended | Not recommended |
Images
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes To detect hEGF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hEGF. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant fragment | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.1-0.2 µg/mL | Notes To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Western blot bands should be around 6.2 kDa. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant fragment | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.5-2 µg/mL | Notes For sandwich ELISA, use this antibody as Detection at 0.5μg/ml with ab18635 as Capture. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant fragment | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant full length protein - Human, Recombinant fragment | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 μg/ml of this antibody is required. |
Species Human | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/ml), a concentration of 0.25 - 0.35 μg/ml of this antibody is required. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant full length protein - Human | Dilution info - | Notes - |
Recommended products
Rabbit Polyclonal EGF antibody. Suitable for ELISA, WB, sELISA, IHC-P, Neut and reacts with Recombinant full length protein, Recombinant full length protein - Human, Human, Recombinant fragment samples. Cited in 34 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EGF.
Alternative names=Pro-epidermal growth factor, EGF, EGF
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
Recombinant Full Length Protein corresponding to Human EGF. Database link: P01133
- Purification technique
Affinity purification Immunogen
- Light chain type
unknown
- Reconstitution
Reconstitute with 200µl of sterile water.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
Notes
Although some customers have been successful in IHC we no longer batch test in this application. For IHC validate antibody please see ab184265.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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