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AB206423

Anti-EGF antibody [EPR19899]

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(7 Publications)

Rabbit Recombinant Monoclonal EGF antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 7 publications.

View Alternative Names

Pro-epidermal growth factor, EGF

3 Images
Western blot - Anti-EGF antibody [EPR19899] (AB206423)
  • WB

Supplier Data

Western blot - Anti-EGF antibody [EPR19899] (AB206423)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 2268351 & 7499272).

All lanes:

Western blot - Anti-EGF antibody [EPR19899] (ab206423) at 1/1000 dilution

All lanes:

Mouse salivary gland lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 133 kDa

Observed band size: 6 kDa

false

Exposure time: 3min

Western blot - Anti-EGF antibody [EPR19899] (AB206423)
  • WB

Supplier Data

Western blot - Anti-EGF antibody [EPR19899] (AB206423)

Blocking and Diluting buffer : 5% NFDM/TBST

Exposure time : 1 second

All lanes:

Western blot - Anti-EGF antibody [EPR19899] (ab206423) at 1/1000 dilution

Lane 1:

Recombinant Human Pro-EGF Protein (aa 21-1023) with His-Tag at 0.015 µg

Lane 2:

Recombinant Human EGF recombinant protein (aa971-1023) at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 133 kDa

false

Western blot - Anti-EGF antibody [EPR19899] (AB206423)
  • WB

Supplier Data

Western blot - Anti-EGF antibody [EPR19899] (AB206423)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-EGF antibody [EPR19899] (ab206423) at 1/1000 dilution

Lane 1:

Human EGF recombinant protein (aa971-1023) at 0.01 µg

Lane 2:

Mouse EGF recombinant protein (aa977-1029) at 0.01 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 133 kDa

Observed band size: 6 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19899

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.
Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed : 10964941).
See full target information EGF
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:15572 PubMed40320451

2025

Unveiling the protective role of ESM1 in endothelial cell proliferation and lipid reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Yukun Li,Anbo Gao,Wenchao Zhou,Xing Tang,Tian Zeng,Tingyu Fan,Weimin Jiang,Min Tang,Fan Ouyang

Life (Basel, Switzerland) 15: PubMed40141816

2025

Role of Bean Yellow Mosaic Virus P1 and HC-Pro in Enhancing Gene Expression and Suppressing RNA Silencing in .

Applications

Unspecified application

Species

Unspecified reactive species

Sunmee Choi,Suk Hyun Kwon,Gi Seok Kwon,Ho Seong Choi,Hyo Hyun Seo,Young Soon Kim,Jeong Hun Lee,Won Kyong Cho,Sang Hyun Moh

Journal of experimental & clinical cancer research : CR 44:68 PubMed39994761

2025

Reciprocal regulation of MMP-28 and EGFR is required for sustaining proliferative signaling in PDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtao Hong,Xing Huang,Linghao Xia,Tingbo Liang,Xueli Bai

BMC cancer 23:840 PubMed37679666

2023

Analysis of myosin genes in HNSCC and identify MYL1 as a specific poor prognostic biomarker, promotes tumor metastasis and correlates with tumor immune infiltration in HNSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Ce Li,Rui Guan,Wenming Li,Dongmin Wei,Shengda Cao,Fen Chang,Qun Wei,Ran Wei,Long Chen,Chenyang Xu,Kainan Wu,Dapeng Lei

Cell death discovery 8:17 PubMed35013115

2022

Tumor-derived extracellular vesicles inhibit HGF/c-Met and EGF/EGFR pathways to accelerate the radiosensitivity of nasopharyngeal carcinoma cells via microRNA-142-5p delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Changyu Zhu,Xiaolei Jiang,Hua Xiao,Jianmei Guan

Cell communication and signaling : CCS 19:53 PubMed33980247

2021

ATF5 and HIF1α cooperatively activate HIF1 signaling pathway in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng He,Hang Xiao,Yixin Cai,Ni Zhang

Cell research : PubMed32839552

2020

Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shen'ao Zhou,Wei Zhang,Gaihong Cai,Yingzhe Ding,Caixia Wei,Sheng Li,Yu Yang,Jie Qin,Dan Liu,Hao Zhang,Xiexiang Shao,Jianhua Wang,Hongye Wang,Wenjun Yang,Huating Wang,She Chen,Ping Hu,Liming Sun
View all publications
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Product promise

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