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AB218831

Anti-EGF antibody [EPR21926]

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(3 Publications)

Rabbit Recombinant Monoclonal EGF antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Rat samples. Cited in 3 publications.

View Alternative Names

Pro-epidermal growth factor, EGF, Egf

5 Images
Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR21926] (AB218831)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-EGF antibody [EPR21926] (AB218831)

Immunohistochemical analysis of frozen rat kidney tissue labeling EGF with ab218831 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on distal convoluted tubule of rat kidney (PMID : 23143303) is observed. Tissue was fixed in 4% PFA and permeabilized using 0.2% Triton X-100.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR21926] (AB218831)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [EPR21926] (AB218831)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling EGF with ab218831 at 1/10,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in distal convoluted tubules of rat kidney (PMID 1930151) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunoprecipitation - Anti-EGF antibody [EPR21926] (AB218831)
  • IP

Supplier Data

Immunoprecipitation - Anti-EGF antibody [EPR21926] (AB218831)

EGF was immunoprecipitated from 0.35 mg of rat salivary gland lysate with ab218831 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218831 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Rat salivary gland tissue 10 μg (Input).

Lane 2 : ab218831 IP in rat salivary gland lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab218831 in rat salivary gland lysate.

Blocking/ Dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-EGF antibody [EPR21926] (ab218831)

Predicted band size: 133 kDa

false

Western blot - Anti-EGF antibody [EPR21926] (AB218831)
  • WB

Supplier Data

Western blot - Anti-EGF antibody [EPR21926] (AB218831)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST

Exposure : Lane 1 : 3 minutes; Lane 2 : 75 seconds

The molecular mass observed is consistent with what has been described in the literature (PMID : 2268351).

All lanes:

Western blot - Anti-EGF antibody [EPR21926] (ab218831) at 1/5000 dilution

Lane 1:

Rat salivary gland tissue lysate at 20 µg

Lane 2:

Rat urine at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 133 kDa

false

Western blot - Anti-EGF antibody [EPR21926] (AB218831)
  • WB

Unknown

Western blot - Anti-EGF antibody [EPR21926] (AB218831)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST

The molecular mass observed is consistent with what has been described in the literature (PMID : 2268351).

All lanes:

Western blot - Anti-EGF antibody [EPR21926] (ab218831) at 1/1000 dilution

All lanes:

Rat EGF active protein (aa974-1026), 20ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 133 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21926

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB, IHC-P, IHC-Fr, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Perform heat-mediated antigen retrieval by sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.
Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
See full target information Egf
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 33:101995 PubMed40688680

2025

Inhibition of gastric adenocarcinoma proliferation by WSGC@MS: Role of KEAP1/NRF2 signaling pathway and autophagy regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Dongjian Song,Qiuliang Liu,Zechen Yan,Qi Wang,Meng Su,Hui Zhang,Longyan Shi,Yingzhong Fan,Qian Zhang,Heying Yang,Da Zhang

Aging 15:13176-13193 PubMed37988198

2023

Investigating EGF and PAG1 as necroptosis-related biomarkers for diabetic nephropathy: an and validation study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuejun Wang,Linlin Zhang,Zhiping Peng

Pharmaceutical biology 61:50-60 PubMed36541204

2022

Gastric protective effect of flavonoids: mediating TLR4/NF-κB and TRPV1 signalling pathways and gastric mucosal healing.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiwen Lin,Tang Deng,Huijuan Qu,Hongya Ou,Qifeng Huang,Bingmiao Gao,Xiaoliang Li,Na Wei
View all publications
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Product promise

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