AB288300

Anti-EGF antibody [RM1015] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Multiclonal EGF antibody. Carrier free. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat samples.

View Alternative Names

Pro-epidermal growth factor, EGF, Egf

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling EGF with ab288294 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on the distal convoluted tubules in mouse kidney. The section was incubated with ab288294 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney (fresh) tissue labeling EGF with ab288294 at 1/500 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green). Positive staining in the renal tubule on the rat kidney is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling EGF with ab288294 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control : no staining on mouse spleen. The section was incubated with ab288294 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling EGF with ab288294 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control : no staining on rat spleen. The section was incubated with ab288294 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling EGF with ab288294 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on the distal convoluted tubules in rat kidney. The section was incubated with ab288294 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling EGF with ab288294 at 1/500 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (Green). Positive staining in the renal tubule on the mouse kidney is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

EGF was immunoprecipitated from 0.35 mg mouse kidney lysate with ab288294 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using aab288294 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse kidney lysate 10μg

Lane 2 : ab288294 IP in Mouse kidney lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab288294 in Mouse kidney lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 23 seconds

All lanes:

Immunoprecipitation - Anti-EGF antibody [RM1015] (<a href='/en-us/products/primary-antibodies/egf-antibody-rm1015-ab288294'>ab288294</a>)

false

Exposure time: 23s

Supplier Data

Immunoprecipitation - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

EGF was immunoprecipitated from 0.35 mg rat salivary gland lysate with ab288294 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288294 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat salivary gland lysate 10μg

Lane 2 : ab288294 IP in Rat salivary gland lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab288294 in rat salivary gland lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 100 seconds.

All lanes:

Immunoprecipitation - Anti-EGF antibody [RM1015] (<a href='/en-us/products/primary-antibodies/egf-antibody-rm1015-ab288294'>ab288294</a>)

false

Exposure time: 100s

Supplier Data

Western blot - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration are 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1636692, 2260661, 7499272 & 2268351).

Freshly made lysate is highly recommended to avoid EGF degradation and for better detection.

All lanes:

Western blot - Anti-EGF antibody [RM1015] (<a href='/en-us/products/primary-antibodies/egf-antibody-rm1015-ab288294'>ab288294</a>) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 20 µg

Lane 2:

Rat salivary gland lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 100s

Supplier Data

Western blot - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

This data was developed using ab288294, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration are 5% NFDM/TBST.

Negative control : Mouse spleen and Rat spleen.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1636692, 2260661, 7499272 & 2268351).

Freshly made lysate is highly recommended to avoid EGF degradation and for better detection.

All lanes:

Western blot - Anti-EGF antibody [RM1015] (<a href='/en-us/products/primary-antibodies/egf-antibody-rm1015-ab288294'>ab288294</a>) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 20 µg

Lane 2:

Mouse spleen lysate at 20 µg

Lane 3:

Rat kidney lysate at 20 µg

Lane 4:

Rat spleen lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3min

Unconjugated

Anti-EGF antibody [RM1015]

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1015

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IHC-Fr, IHC-P, IP

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

ab288300 is a carrier free version of ab288294.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.

Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).

See full target information Egf

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EGF antibody [RM1015] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Immunohistochemistry (Frozen sections) - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Immunoprecipitation - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Immunoprecipitation - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Western blot - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Western blot - Anti-EGF antibody [RM1015] - BSA and Azide free (AB288300)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise