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AB276159

Anti-EGFL7 antibody [EPR22603-190] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal EGFL7 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

MEGF7, UNQ187/PRO1449, EGFL7, Epidermal growth factor-like protein 7, EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, NOTCH4-like protein, Vascular endothelial statin, Zneu1, Multiple EGF-like domains protein 7, VE-statin

1 Images
Sandwich ELISA - Anti-EGFL7 antibody [EPR22603-190] - BSA and Azide free (Capture) (AB276159)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-EGFL7 antibody [EPR22603-190] - BSA and Azide free (Capture) (AB276159)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab275896).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22603-190

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab276159 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human EGFL7. The recommended pair for sandwich ELISA is:
Capture: ab276159, Human EGFL7 Capture Antibody (unconjugated)
Detector: ab276160, Human EGFL7 Detector Antibody (unconjugated)
The reference range value is 0.469 - 30 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFL7 also known as Epidermal Growth Factor-like Domain 7 is a secreted protein with a molecular mass of approximately 31 kDa. Scientists observe its expression mainly in vascular endothelial cells indicating its role in vasculature. It characterizes itself by its distinctive EGF-like repeat domains which enable interaction with other proteins in the cellular environment.
Biological function summary

EGFL7 plays an essential role in blood vessel development and maintenance. It is involved in the modulation of endothelial cell behavior primarily through cell proliferation migration and organization. EGFL7 influences angiogenesis by affecting the extracellular matrix environment but it does not form part of a larger protein complex. It serves as a regulator by interacting directly with other components of the angiogenic process.

Pathways

Research has established EGFL7 as a significant participant in the angiogenesis and Notch signaling pathways. It interacts closely with proteins such as COX-2 within the angiogenic pathway and through its efficacy impacts the downstream signaling events in vessels' formation and repair. The proper functioning of these pathways highlights the importance of EGFL7 in vascular biology.

Researchers associate EGFL7 with certain cancers and cardiovascular diseases. Elevated levels of EGFL7 in tumors implicate it in tumor angiogenesis and metastasis suggesting its potential as a biomarker or therapeutic target. In cardiovascular contexts its interaction with VEGF suggests a role in pathologies involving vascular dysfunctions including atherosclerosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.
See full target information EGFL7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com