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AB218383

Anti-EGFR antibody [31G7]

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(3 Publications)

Anti-EGFR antibody [31G7] (ab218383) is a mouse monoclonal antibody detecting EGFR in Flow Cytometry, ICC/IF. Suitable for Human.

View Alternative Names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

2 Images
Flow Cytometry - Anti-EGFR antibody [31G7] (AB218383)
  • Flow Cyt

Lab

Flow Cytometry - Anti-EGFR antibody [31G7] (AB218383)

Flow Cytometry analysis of A431 (human epidermoid carcinoma epithelial cell line) cells (Right image) labeling EGFR with ab218383 at 1/900 dilution. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue).

Negative control : SW620 (human colorectal adenocarcinoma epithelial cell line) (Left image)
Gated on viable cells.

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [31G7] (AB218383)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [31G7] (AB218383)

Immunocytochemistry analysis of A431 (human epidermoid carcinoma epithelial cell line) cells labeling EGFR with ab218383 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-beta Tubulin rabbit monoclonal antibody (ab179593) at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) secondary antibody at (ab179513) at 1/500 dilution (red). Nuclear DNA was labelled with DAPI (blue).

Negative control : SW620 (Human colorectal adenocarcinoma epithelial cells) (PMID : 19789347)

  • Carrier free

    Anti-EGFR antibody [31G7] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

31G7

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/900", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-EGFR antibody [31G7] (ab218383) is a mouse recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Other related products
We have a range of other formats of antibody clone [31G7] also available for your convenience: ab289889, Carrier free - ab289896, Carrier free - ab212335

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
See full target information EGFR
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7671 PubMed36509746

2022

An atlas of late prenatal human neurodevelopment resolved by single-nucleus transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Susana I Ramos,Zarmeen M Mussa,Elisa N Falk,Balagopal Pai,Bruno Giotti,Kimaada Allette,Peiwen Cai,Fumiko Dekio,Robert Sebra,Kristin G Beaumont,Alexander M Tsankov,Nadejda M Tsankova

Pathology, research and practice 213:1530-1535 PubMed29108919

2017

EGFR immunohistochemistry as biomarker for antibody-based therapy of squamous NSCLC - Experience from the first ring trial of the German Quality Assurance Initiative for Pathology (QuIP).

Applications

Unspecified application

Species

Unspecified reactive species

Iver Petersen,Manfred Dietel,Wolf J Geilenkeuser,Masoud Mireskandari,Wilko Weichert,Katja Steiger,Andreas H Scheel,Reinhard Büttner,Peter Schirmacher,Arne Warth,Felix Lasitschka,Hans-Ulrich Schildhaus,Thomas Kirchner,Simone Reu,Hans Kreipe,Florian Länger,Markus Tiemann,Christoph Schulte,Korinna Jöhrens

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 80:146-55 PubMed27127342

2016

Breed- and age-related differences in canine mammary tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Woo Kim,Ha-Young Lim,Jong-Il Shin,Byung-Joon Seung,Jung-Hyung Ju,Jung-Hyang Sur
View all publications
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