Anti-EGFR antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody (AB245363)

Formalin-fixed, paraffin-embedded human placenta tissue stained for EGFR with ab245363 at a 1/1,000 dilution in immunohistochemical analysis. DAB staining.

• IP

Supplier Data

Immunoprecipitation - Anti-EGFR antibody (AB245363)

EGFR was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab245363 used for IP at 6 μg per reaction. For WB 0.1 μg/ml.

Lane 1 : ab245363 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 3 seconds.

All lanes:

Immunoprecipitation - Anti-EGFR antibody (ab245363)

Predicted band size: 134 kDa

false

• WB

Supplier Data

Western blot - Anti-EGFR antibody (AB245363)

Prepared using NETN lysis buffer.

Detection : Chemiluminescence.

All lanes:

Western blot - Anti-EGFR antibody (ab245363) at 0.04 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 134 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-EGFR antibody (AB245363)

Prepared using NETN lysis buffer.

Detection : Chemiluminescence.

All lanes:

Western blot - Anti-EGFR antibody (ab245363) at 0.04 µg/mL

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 134 kDa

false

Exposure time: 30s