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AB30

Anti-EGFR antibody [EGFR1]

4

(4 Reviews)

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(50 Publications)

Anti-EGFR antibody [EGFR1] (ab30) is a mouse monoclonal antibody detecting EGFR in Flow Cytometry, IHC-Fr, ICC/IF. Suitable for Human.

- Over 30 publications
- Trusted since 1998

View Alternative Names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

3 Images
Flow Cytometry - Anti-EGFR antibody [EGFR1] (AB30)
  • Flow Cyt

Lab

Flow Cytometry - Anti-EGFR antibody [EGFR1] (AB30)

Overlay histograms showing left positive A431 cells and right negative Jurkat cells stained with ab30 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab30, 1x106 cells in 100ul at 1ug/ml) for 30 min on ice. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) preabsorbed (ab150117) at 1/2000 dilution for 30 min on ice.

Isotype control antibody (black line) was mouse IgG2bκ (ab170192) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

Immunohistochemistry (Frozen sections) - Anti-EGFR antibody [EGFR1] (AB30)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-EGFR antibody [EGFR1] (AB30)

IHC image of EGFR staining in a section of frozen normal human placenta, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab30, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EGFR1] (AB30)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EGFR1] (AB30)

ab30 staining EGFR in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab30 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • 778 PE/Cy7®

    PE/Cy7® Anti-EGFR antibody [EGFR1]

  • 578 PE

    PE Anti-EGFR antibody [EGFR1]

  • Carrier free

    Anti-EGFR antibody [EGFR1] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EGFR1

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-Fr, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Extracellular epitope mapped between aa 6-273 of human EGFR.

Specificity

Recognises the external EGF-binding domain of the EGFR transmembrane glycoprotein. No effect on tyrosine kinase activity of EGFR.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "10 µg/mL", "IHCFr-species-notes": "<p></p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Horse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-EGFR antibody [EGFR1] (ab30) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-EGFR [EGFR1] (ab30) was first used in a scientific publication in 1998 and has been cited over 30 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [EGFR1] also available for your convenience: ab30, PE - ab130738, ab239309, Carrier free - ab264523, Alexa Fluor® 647 - ab320714

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
See full target information EGFR
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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:55 PubMed39972345

2025

EGFR-to-Src family tyrosine kinase switching in proliferating-DTP TNBC cells creates a hyperphosphorylation-dependent vulnerability to EGFR TKI.

Applications

Unspecified application

Species

Unspecified reactive species

Nazia Chaudhary,Bhagya Shree Choudhary,Anusha Shivashankar,Subhakankha Manna,Khyati Ved,Shagufa Shaikh,Sonal Khanna,Jeetnet Baar,Jagruti Dani,Sarthak Sahoo,R Soundharya,Mohit Kumar Jolly,Nandini Verma

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405459 PubMed39206796

2024

Genomic Amplification of TBC1D31 Promotes Hepatocellular Carcinoma Through Reducing the Rab22A-Mediated Endolysosomal Trafficking and Degradation of EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Pengbo Cao,Hongxia Chen,Ying Zhang,Qi Zhang,Mengting Shi,Huihui Han,Xiaowen Wang,Liang Jin,Bingqian Guo,Rongjiao Hao,Xi Zhao,Yuanfeng Li,Chengming Gao,Xinyi Liu,Yahui Wang,Aiqing Yang,Chenning Yang,Anfeng Si,Hua Li,Qingfeng Song,Fuchu He,Gangqiao Zhou

iScience 27:110417 PubMed39108703

2024

TSPAN4 influences glioblastoma progression through regulating EGFR stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbin Dong,Xiaolong Tang,Wenhui Zhao,Ping Liu,Weiru Yu,Jinlai Ren,Yu Chen,Yanfang Cui,Juan Chen,Yongshuo Liu

Asian Pacific journal of cancer prevention : APJCP 25:2193-2201 PubMed38918683

2024

Evaluation of the Expression EGFR, HER2/NEU and the End Effector ERK of the RAS/RAF/MAP Kinase Pathway in Prostatic Adenocarcinoma for a Possible Role as New Target Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Asem Shalaby,Heba A Mahmoud,Shayma G Sayed,Khalid Al Hashmi,Shadia Al-Sinawi,Suad Al Badi,Afrah Al Rashdi,Samya Al Husaini,Hajer Al Badi,Somaia Ahmed Saad El-Din

Cells 13: PubMed38667283

2024

Reversal of Postnatal Brain Astrocytes and Ependymal Cells towards a Progenitor Phenotype in Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitrios Kakogiannis,Michaela Kourla,Dimitrios Dimitrakopoulos,Ilias Kazanis

The Journal of cell biology 223: PubMed38240798

2024

Single-molecule imaging of stochastic interactions that drive dynein activation and cargo movement in cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nireekshit Addanki Tirumala,Gregory Michael Ian Redpath,Sarah Viktoria Skerhut,Pritha Dolai,Natasha Kapoor-Kaushik,Nicholas Ariotti,K Vijay Kumar,Vaishnavi Ananthanarayanan

Virology 590:109952 PubMed38103269

2023

Epidermal growth factor receptor-dependent stimulation of differentiation by human papillomavirus type 16 E5.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Trammel,Oluwamuyiwa Amusan,Allison Hultgren,Gaurav Raikhy,Jason M Bodily

Nature communications 14:7859 PubMed38030597

2023

Endosomal Arl4A attenuates EGFR degradation by binding to the ESCRT-II component VPS36.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Jin Lin,Ming-Chieh Lin,Tsai-Jung Liu,Yueh-Tso Tsai,Ming-Ting Tsai,Fang-Jen S Lee

Methods in molecular biology (Clifton, N.J.) 2668:257-276 PubMed37140802

2023

Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Bernuz,Arnau Pallarès-Rusiñol,Rosanna Rossi,Carolina Fernández-Senac,Mercè Martí,María Isabel Pividori

International journal of molecular sciences 23: PubMed36555466

2022

Expanding the Therapeutic Window of EGFR-Targeted PE24 Immunotoxin for EGFR-Overexpressing Cancers by Tailoring the EGFR Binding Affinity.

Applications

Unspecified application

Species

Unspecified reactive species

Sei-Yong Jun,Dae-Seong Kim,Yong-Sung Kim
View all publications
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Product promise

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