Rabbit Recombinant Monoclonal EGFR antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 6 publications.
Images
![Western blot - Anti-EGFR antibody [EPR39Y] (AB76153), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-epr39y-ab76153--western-blot-img101276.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EGFR antibody [EPR39Y] (AB76153)")
![Flow Cytometry (Intracellular) - Anti-EGFR antibody [EPR39Y] (AB76153), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-epr39y-ab76153--flow-cytometry-intracellular-img22307.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-EGFR antibody [EPR39Y] (AB76153)")
Publications
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- Small (Weinheim an der Bergstrasse, Germany) 18:e220448520223D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNastaran Barin,Hayri E Balcioglu,Iris de Heer,Maurice de Wit,Martine L M Lamfers,Martin E van Royen,Pim J French,Angelo AccardoPubMed 36207287
- Journal of cellular biochemistry 122:1277-12942021SENP1 participates in Irinotecan resistance in human colon cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Cheng Chen,Do Chi Nhan,Chiung-Hung Hsu,Tso-Fu Wang,Chi-Cheng Li,Tsung-Jung Ho,B Mahalakshmi,Mei-Chih Chen,Liang-Yo Yang,Chih-Yang HuangPubMed 34037277
- Autophagy 16:435-4502019Inactivation of MTOR promotes autophagy-mediated epithelial injury in particulate matter-induced airway inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin-Fang Wu,Zhou-Yang Li,Ling-Ling Dong,Wei-Jie Li,Yan-Ping Wu,Jing Wang,Hai-Pin Chen,Hui-Wen Liu,Miao Li,Ci-Liang Jin,Hua-Qiong Huang,Song-Min Ying,Wen Li,Hua-Hao Shen,Zhi-Hua ChenPubMed 31203721
- PloS one 13:e01931702018Vitamin C alters the amount of specific endoplasmic reticulum associated proteins involved in lipid metabolism in the liver of mice synthesizing a nonfunctional Werner syndrome (Wrn) mutant protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucie Aumailley et. al.PubMed 29494634
- Scientific reports 7:142222017The role of Thyroid Transcription Factor-1 and Tumor differentiation in Resected Lung Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsai-Wang Huang et. al.PubMed 29079814
- Oncology letters 10:1495-15002015RNA interference for epidermal growth factor receptor enhances the radiosensitivity of esophageal squamous cell carcinoma cell line Eca109.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Not recommended | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR. |
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR. |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Species Mouse | Dilution info 1/100 - 1/250 | Notes - |
Flow Cyt (Intra)
TestedTested
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Recommended products
Rabbit Recombinant Monoclonal EGFR antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR39Y
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody detects both phosphorylated and non-phosphorylated EGFR protein. This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
- Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
- Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
- Associated diseases and disorders
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-EGFR antibody [EPR39Y] (ab76153), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-epr39y-ab76153--western-blot-img101276.jpg "Western blot - Anti-EGFR antibody [EPR39Y] (ab76153)")
Western blot - Anti-EGFR antibody [EPR39Y] (ab76153)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab76153 overnight at 4°C in the presence of loading control ab18058 (Mouse monoclonal [SPM227] to Vinculin diluted 1:10000). Antibody binding was detected using IR-labelled goat anti-Rabbit Ab at a 1:10,000 dilution for one hour at room temperature before imaging.
This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR.
All lanes: Western blot - Anti-EGFR antibody [EPR39Y] (ab76153) at 1/1000 dilution
Lane 1: Caco-2 cell lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
Lane 3: Mouse skin cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 134 kDa
![Flow Cytometry (Intracellular) - Anti-EGFR antibody [EPR39Y] (ab76153), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-epr39y-ab76153--flow-cytometry-intracellular-img22307.jpg "Flow Cytometry (Intracellular) - Anti-EGFR antibody [EPR39Y] (ab76153)")
Flow Cytometry (Intracellular) - Anti-EGFR antibody [EPR39Y] (ab76153)
Overlay histogram showing A431 cells stained with ab76153 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76153, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EPR39Y] (ab76153), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-epr39y-ab76153--immunocytochemistry-immunofluorescence-img63504.gif "Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EPR39Y] (ab76153)")
Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EPR39Y] (ab76153)
ab76153 at 1/100 dilution staining EGFR in A431 cells by Immunofluorescence.
![Western blot - Anti-EGFR antibody [EPR39Y] (ab76153), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-epr39y-ab76153--western-blot-img89943.gif "Western blot - Anti-EGFR antibody [EPR39Y] (ab76153)")
Western blot - Anti-EGFR antibody [EPR39Y] (ab76153)
All lanes: Western blot - Anti-EGFR antibody [EPR39Y] (ab76153) at 1/5000 dilution
All lanes: HeLa cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 134 kDa
Observed band size: 170 kDa, 180 kDa
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