Anti-EGFR antibody [ICR10] (ab231) is a rat monoclonal antibody detecting EGFR in Flow Cytometry, ICC/IF. Suitable for Human.
- Over 30 publications
- Trusted since 1999
Images
![Flow Cytometry - Anti-EGFR antibody [ICR10] (AB231), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-icr10-ab231--flow-cytometry-img446867.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-EGFR antibody [ICR10] (AB231)")
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (AB231), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-icr10-ab231--immunocytochemistry-immunofluorescence-img446772.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (AB231)")
Publications
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- Cells 12:2023Dual Targeting of EGFR and MTOR Pathways Inhibits Glioblastoma Growth by Modulating the Tumor Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaxim Sidorov,Pratiksha Dighe,Rinette W L Woo,Aida Rodriguez-Brotons,Michelle Chen,Ryan J Ice,Edith Vaquero,Damon Jian,Pierre-Yves Desprez,Mehdi Nosrati,Leah Galvez,Lewis Leng,Lawrence Dickinson,Mohammed Kashani-Sabet,Sean David McAllister,Liliana SoroceanuPubMed 36831214
- STAR protocols 3:1014752022Measuring EGFR plasma membrane density, stability, internalization, and recycling in alive adherent cells by cell surface ELISA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJalal M Kazan,Ariadna Pollato-Blanco,Gergely L Lukacs,Arnim PausePubMed 35755125
- IEEE open journal of engineering in medicine and biology 3:86-952022Assessment of CAR-T Cell-Mediated Cytotoxicity in 3D Microfluidic Cancer Co-Culture Models for Combination Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarla Paterson,Sarah Paterson,Theresa Mulholland,Seth B Coffelt,Michele ZagnoniPubMed 35813488
- Nature cancer 3:418-4362022Functional patient-derived organoid screenings identify MCLA-158 as a therapeutic EGFR × LGR5 bispecific antibody with efficacy in epithelial tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBram Herpers,Berina Eppink,Mark I James,Carme Cortina,Adrià Cañellas-Socias,Sylvia F Boj,Xavier Hernando-Momblona,Dominik Glodzik,Rob C Roovers,Marc van de Wetering,Carina Bartelink-Clements,Vanessa Zondag-van der Zande,Jara García Mateos,Kuan Yan,Lucia Salinaro,Abdul Basmeleh,Szabolcs Fatrai,David Maussang,Jeroen J Lammerts van Bueren,Irene Chicote,Garazi Serna,Laia Cabellos,Lorena Ramírez,Paolo Nuciforo,Ramon Salazar,Cristina Santos,Alberto Villanueva,Camille Stephan-Otto Attolini,Elena Sancho,Hector G Palmer,Josep Tabernero,Michael R Stratton,John de Kruif,Ton Logtenberg,Hans Clevers,Leo S Price,Robert G J Vries,Eduard Batlle,Mark ThrosbyPubMed 35469014
- International journal of molecular sciences 22:2021Two-Chains Tissue Plasminogen Activator Unifies Met and NMDA Receptor Signalling to Control Neuronal Survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElodie Hedou,Sara Douceau,Arnaud Chevilley,Alexandre Varangot,Audrey M Thiebaut,Hortense Triniac,Isabelle Bardou,Carine Ali,Mike Maillasson,Tiziana Crepaldi,Paolo Comoglio,Eloïse Lemarchand,Véronique Agin,Benoit D Roussel,Denis VivienPubMed 34948279
- iScience 24:1032742021Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJalal M Kazan,Guillaume Desrochers,Claire E Martin,Hyeonju Jeong,Dmitri Kharitidi,Pirjo M Apaja,Ariel Roldan,Nicole St Denis,Anne-Claude Gingras,Gergely L Lukacs,Arnim PausePubMed 34761192
- Cell 184:5053-5069.e232021Chromatin and gene-regulatory dynamics of the developing human cerebral cortex at single-cell resolution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandro E Trevino,Fabian Müller,Jimena Andersen,Laksshman Sundaram,Arwa Kathiria,Anna Shcherbina,Kyle Farh,Howard Y Chang,Anca M Pașca,Anshul Kundaje,Sergiu P Pașca,William J GreenleafPubMed 34390642
- Cell reports 34:1087882021Heterogeneity of glial progenitor cells during the neurogenesis-to-gliogenesis switch in the developing human cerebral cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Fu,Ming Yang,Hongmin Yu,Yicheng Wang,Xinglong Wu,Jun Yong,Yunuo Mao,Yueli Cui,Xiaoying Fan,Lu Wen,Jie Qiao,Fuchou TangPubMed 33657375
- Cell reports 31:1075992020Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicola Micali,Suel-Kee Kim,Marcelo Diaz-Bustamante,Genevieve Stein-O'Brien,Seungmae Seo,Joo-Heon Shin,Brian G Rash,Shaojie Ma,Yanhong Wang,Nicolas A Olivares,Jon I Arellano,Kristen R Maynard,Elana J Fertig,Alan J Cross,Roland W Bürli,Nicholas J Brandon,Daniel R Weinberger,Joshua G Chenoweth,Daniel J Hoeppner,Nenad Sestan,Pasko Rakic,Carlo Colantuoni,Ronald D McKayPubMed 32375049
- Scientific reports 9:146112019CD206 tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA S M Rafiul Haque et. al.PubMed 31601953
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human EGFR. Database link P00533
Reactivity data
Select an application| Flow Cyt | ICC/IF | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Recommended products
Anti-EGFR antibody [ICR10] (ab231) is a rat monoclonal antibody detecting EGFR in Flow Cytometry, ICC/IF. Suitable for Human.
- Over 30 publications
- Trusted since 1999
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human EGFR. Database link P00533
- Clone number
- ICR10
- Purification technique
- Ion exchange chromatography
- Epitope
- ICR10 binds to epitope B, and has an affinity of 6.7x10-9 M.
- Light chain type
- unknown
- Concentration
- Purification notes
Purified IgG prepared by ion exchange chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-EGFR antibody [ICR10] (ab231) is a rat monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Trusted by the scientific community
Anti-EGFR [ICR10] (ab231) was first used in a scientific publication in 1999 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-EGFR [ICR10] (ab231) has over 5 independent reviews from customers.
Other related products
We have a range of other formats of antibody clone [ICR10] also available for your convenience:
ab231, FITC - FITC Anti-EGFR antibody [ICR10] ab11400, PE - PE Anti-EGFR antibody [ICR10] ab27764
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
- Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
- Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
- Associated diseases and disorders
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-icr10-ab231--flow-cytometry-img446867.jpg "Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)")
Flow Cytometry - Anti-EGFR antibody [ICR10] (ab231)
EGFR Flow Cytometry staining of A431 (Human epidermoid carcinoma epithelial cell) cells using rat Anti-EGFR antibody
Flow cytometric analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling EGFR with ab231 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) / Blue.
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 647, ab150163) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-icr10-ab231--immunocytochemistry-immunofluorescence-img446772.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231)")
Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [ICR10] (ab231)
EGFR Immunocytochemistry/ Immunofluorescence staining of A431 cells using rat Anti-EGFR antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling EGFR with ab231 at 1/50 dilution followed by secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (green).
Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) used at a 1/1000 dilution (red).
Confocal image showing strong membranous and weak cytoplasmic staining in A431 cells.
Low expression control: HEK-293 (PMID: 26368334).Nuclear DNA was labelled with DAPI (shown in blue).
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