Anti-EGFR antibody [SP84] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human piver tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227642).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human placenta tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Immunocytochemistry/ Immunofluorescence analysis of A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1 : 50 (1.92 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227737).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Flow Cytometry analysis of A431(Human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1 : 200 dilution (0.48 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227635).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

• WB

Lab

Western blot - Anti-EGFR antibody [SP84] - BSA and Azide free (AB238803)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227642).

Western blot : Anti-EGFR antibody [SP84] - C-terminal ab227642 staining at 1/200 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 160 kDa in Wild-type A549 cell lysates with no signal observed at this size in EGFR knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-EGFR antibody [SP84] - C-terminal (<a href='/en-us/products/primary-antibodies/egfr-antibody-sp84-c-terminal-ab227642'>ab227642</a>) at 1/200 dilution

Lane 1:

Wild-type A549 cell lysates at 20 µg

Lane 2:

EGFR knockout A549 cell lysates at 20 µg

Lane 3:

Wild-type HeLa ab255928 cell lysates at 20 µg

Lane 4:

Western blot - Human EGFR knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-egfr-knockout-hela-cell-line-ab255385'>ab255385</a>) at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 134 kDa

Observed band size: 160 kDa

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