Anti-EGFR antibody [SP84] - C-terminal (ab227642) is a rabbit monoclonal antibody detecting EGFR in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (AB227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94363.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (AB227642)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (AB227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136656.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (AB227642)")
![Flow Cytometry (Intracellular) - Anti-EGFR antibody [SP84] - C-terminal (AB227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--flow-cytometry-intracellular-img26228.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-EGFR antibody [SP84] - C-terminal (AB227642)")
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - C-terminal (AB227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunocytochemistry-immunofluorescence-img72389.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - C-terminal (AB227642)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (AB227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133963.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (AB227642)")
Publications
Filter by
- Lung cancer (Amsterdam, Netherlands) 106:131-1372017MET exon 14 skipping mutation in triple-negative pulmonary adenocarcinomas and pleomorphic carcinomas: An analysis of intratumoral MET status heterogeneity and clinicopathological characteristics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDohee Kwon,Jaemoon Koh,Sehui Kim,Heounjeong Go,Young A Kim,Bhumsuk Keam,Tae Min Kim,Dong-Wan Kim,Yoon Kyung Jeon,Doo Hyun ChungPubMed 28285687
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Primary antibody incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25 | Notes Primary antibody incubation for 1 hour at room temperature. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes Permeabilize with 90% Methanol (-20 °C for 30min) after fixation. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Recommended products
Anti-EGFR antibody [SP84] - C-terminal (ab227642) is a rabbit monoclonal antibody detecting EGFR in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP84
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-EGFR antibody [SP84] - C-terminal (ab227642) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of EGFR?
Anti-EGFR [SP84] - C-terminal (ab227642) specifically detects a band for EGFR (UniProt: P07327) at a molecular weight of 134kDa.
Collaboration
Anti-EGFR [SP84] - C-terminal (ab227642) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Other related products
We have a range of other formats of antibody clone [SP84] also available for your convenience:
ab227642, Carrier free - Anti-EGFR antibody [SP84] - BSA and Azide free ab238803, ab279512, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-EGFR antibody [SP84] - C-terminal ab320599, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-EGFR antibody [SP84] - C-terminal ab320600, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-EGFR antibody [SP84] - C-terminal ab320601, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-EGFR antibody [SP84] - C-terminal ab320602, PE - PE Anti-EGFR antibody [SP84] - C-terminal ab320603, APC - APC Anti-EGFR antibody [SP84] - C-terminal ab320604, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-EGFR antibody [SP84] - C-terminal ab321433
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
- Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
- Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
- Associated diseases and disorders
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
14 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img94363.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136656.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Flow Cytometry (Intracellular) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--flow-cytometry-intracellular-img26228.jpg "Flow Cytometry (Intracellular) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Flow Cytometry (Intracellular) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Flow Cytometry analysis of A431(Human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1:200 dilution (0.48 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunocytochemistry-immunofluorescence-img72389.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Immunocytochemistry/ Immunofluorescence analysis of A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1:50 (1.92 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133963.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87753.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130181.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104482.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124036.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136796.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109945.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136646.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human placenta tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125725.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
Formalin-fixed, paraffin-embedded human piver tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
![Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--western-blot-img11430.jpg "Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
All lanes: Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642) at 1/25 dilution
All lanes: A431 (human epidermoid carcinoma cell line) EGF treated cell lysate
Predicted band size: 134 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87753.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130181.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104482.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124036.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136796.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109945.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136646.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125725.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
![Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642), expandable thumbnail](https://content.abcam.com/products/images/egfr-antibody-sp84-c-terminal-ab227642--western-blot-img11430.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642)")
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