Anti-EGFR antibody [SP84] - C-terminal (ab227642) is a rabbit monoclonal antibody detecting EGFR in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Primary antibody incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/25 | Notes Primary antibody incubation for 1 hour at room temperature. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200 | Notes Permeabilize with 90% Methanol (-20 °C for 30min) after fixation. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow, Pig | Dilution info - | Notes - |
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The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Anti-EGFR antibody [SP84] - C-terminal (ab227642) is a rabbit monoclonal antibody detecting EGFR in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Purified from TCS by protein A/G.
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry analysis of A431(Human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1:200 dilution (0.48 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
Immunocytochemistry/ Immunofluorescence analysis of A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1:50 (1.92 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human placenta tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human piver tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.
All lanes: Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642) at 1/25 dilution
All lanes: A431 (human epidermoid carcinoma cell line) EGF treated cell lysate
Predicted band size: 134 kDa
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