Rabbit Recombinant Monoclonal EGFR phospho S1046 + S1047 antibody. Carrier free. Suitable for IP, Dot, WB, ICC/IF and reacts with Human, Synthetic peptide samples.
Images
![Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (AB256225), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-s1046-s1047-antibody-ep2259y-bsa-and-azide-free-ab256225--immunoprecipitation-img5626.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (AB256225)")
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (AB256225), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-s1046-s1047-antibody-ep2259y-bsa-and-azide-free-ab256225--immunocytochemistry-immunofluorescence-img36187.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (AB256225)")
![Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (AB256225), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-s1046-s1047-antibody-ep2259y-bsa-and-azide-free-ab256225--dot-blot-img137430.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (AB256225)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Expected | Not recommended | Tested |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:10805725, PubMed:27153536, PubMed:2790960, PubMed:35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:12297049, PubMed:15611079, PubMed:17909029, PubMed:20837704, PubMed:27153536, PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity). Isoform 2 may act as an antagonist of EGF action. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
Alternative names
ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Recommended products
Rabbit Recombinant Monoclonal EGFR phospho S1046 + S1047 antibody. Carrier free. Suitable for IP, Dot, WB, ICC/IF and reacts with Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP2259Y
- Purification technique
- Affinity purification Protein A
- Specificity
Recognises EGFR phosphorylated on Serine 1046 and Serine 1047 of the mature human isoform 1 (corresponding to S1070 and S1071 from the precursor form P00533-1/p170)
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab256225 is the carrier-free version of Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
- Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
- Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
- Associated diseases and disorders
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-s1046-s1047-antibody-ep2259y-bsa-and-azide-free-ab256225--immunoprecipitation-img5626.jpg "Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)")
Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)
This data was developed using Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300, the same antibody clone in a different buffer formulation.
Purified Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300 at 1/40 dilution (2μg) immunoprecipitating EGFR in A431 treated with EGF whole cell lysate.
Lane 1 (input): A431 (Human epidermoid carcinoma epithelial cell) treated with EGF whole cell lysate 10μg
Lane 2 (+): Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300 + A431 treated with EGF whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300 in A431 treated with EGF whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 180 kDaAll lanes: Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] (Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300)
Predicted band size: 134 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-s1046-s1047-antibody-ep2259y-bsa-and-azide-free-ab256225--immunocytochemistry-immunofluorescence-img36187.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)")
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)
Immunocytochemistry/Immunofluorescence analysis of untreated and EGF (100ng/mL for 10 minutes) treated A431 cells labelling EGFR (phospho S1046 + S1047) with Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, an Alexa Fluor® 594-conjugated mouse anti-tubulin (1/200). Nuclei counterstained with DAPI (blue).
Control 1: untreated A431 cells incubated with PBS instead of primary antibody followed by incubation with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 goat anti-rabbit IgG.
Control 2: EGF treated A431 cells incubated with PBS instead of primary antibody followed by incubation with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 goat anti-rabbit IgG.This data was developed using the same antibody clone in a different buffer formulation containing Tris Glycine, BSA, glycerol, and sodium azide (Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300).
![Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-s1046-s1047-antibody-ep2259y-bsa-and-azide-free-ab256225--dot-blot-img137430.jpg "Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)")
Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)
This data was developed using the same antibody clone in a different buffer formulation (Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300).
Primary antibody: Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] ab76300 at a dilution of 1/1000.
Secondary antibody: Peroxidase conjugated-goat anti-rabbit IgG, (H+L) at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST
Lane 1: EGFR (pS1046/pS1047) phospho peptide.
Lane 2: EGFR non-phospho peptide.Exposure time: 3 minutes.
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