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AB5650

Anti-EGFR (phospho Y1086) antibody

4

(1 Review)

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(14 Publications)

Rabbit Polyclonal EGFR phospho Y1086 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human EGFR phospho Y1086.

View Alternative Names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

3 Images
Immunocytochemistry - Anti-EGFR (phospho Y1086) antibody (AB5650)
  • ICC

Supplier Data

Immunocytochemistry - Anti-EGFR (phospho Y1086) antibody (AB5650)

A-431 cells stained for EGFR (green) using ab5650 at 1/100 dilution in ICC/IF. Followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing membranous localization. Panel e represents cells treated with antagonist, Afatinib (1uM for 6hrs) followed by EGF (200 ng/mL for 10 minutes), showing no signal. Panel f shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background.

Western blot - Anti-EGFR (phospho Y1086) antibody (AB5650)
  • WB

Supplier Data

Western blot - Anti-EGFR (phospho Y1086) antibody (AB5650)

All lanes:

Western blot - Anti-EGFR (phospho Y1086) antibody (ab5650) at 1/1000 dilution

Lane 1:

A549 cell lysate at 30 µg

Lane 2:

A549 cell lysate treated for 10 minutes with 200 ng/mL of EGF at 30 µg

Lane 3:

A431 cell lysate at 30 µg

Lane 4:

A431 cell lysate treated for 10 minutes with 200 ng/mL of EGF at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/5000 dilution

Predicted band size: 134 kDa

false

Western blot - Anti-EGFR (phospho Y1086) antibody (AB5650)
  • WB

Supplier Data

Western blot - Anti-EGFR (phospho Y1086) antibody (AB5650)

All lanes:

Western blot - Anti-EGFR (phospho Y1086) antibody (ab5650) at 1/1000 dilution

Lane 1:

A-431 extracts at 30 µg

Lane 2:

A-431 treated with EGF (200 ng/mL for 10 minutes) at 30 µg

Lane 3:

A-431 treated with Gefitinib followed by EGF (1uM for 16 hours, 200 ng/mL for 10 minutes) at 30 µg

Lane 4:

A-431 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200 ng/mL for 10 minutes) at 30 µg

Lane 5:

A549 extracts at 30 µg

Lane 6:

A549 treated with EGF (200 ng/mL for 10 minutes) at 30 µg

Lane 7:

A549 treated with Afatinib followed by EGF (0.5 uM for 6 hours, 200 ng/mL for 10 minutes) at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human EGFR phospho Y1086. The exact immunogen used to generate this antibody is proprietary information.

P00533

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody has been negatively preadsorbed using (i) a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated epidermal growth factor receptor (EGFR), and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an EGFR-derived peptide that is phosphorylated at tyrosine 1086.
Storage buffer
pH: 7.3 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
See full target information EGFR phospho Y1086
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 10:648-661 PubMed32195033

2020

Blocking c-Met and EGFR reverses acquired resistance of PARP inhibitors in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Yi Chu,Clinton Yam,Mei-Kuang Chen,Li-Chuan Chan,Min Xiao,Yong-Kun Wei,Hirohito Yamaguchi,Pei-Chih Lee,Ye Han,Lei Nie,Xian Sun,Stacy L Moulder,Kenneth R Hess,Bin Wang,Jennifer L Hsu,Gabriel N Hortobagyi,Jennifer Litton,Jeffrey T Chang,Mien-Chie Hung

American journal of cancer research 9:180-185 PubMed30755821

2019

An essential role of PRMT1-mediated EGFR methylation in EGFR activation by ribonuclease 5.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Jan Wang,Jung-Mao Hsu,Ying-Nai Wang,Heng-Huan Lee,Hirohito Yamaguchi,Hsin-Wei Liao,Mien-Chie Hung

Cancer research 79:819-829 PubMed30573522

2018

EGFR and c-MET Cooperate to Enhance Resistance to PARP Inhibitors in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiongzhu Dong,Yi Du,Hui Li,Chunxiao Liu,Yongkun Wei,Mei-Kuang Chen,Xixi Zhao,Yu-Yi Chu,Yufan Qiu,Lunxiu Qin,Hirohito Yamaguchi,Mien-Chie Hung

Cancer cell 34:954-969.e4 PubMed30537515

2018

Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Chih Lee,Yueh-Fu Fang,Hirohito Yamaguchi,Wei-Jan Wang,Tse-Ching Chen,Xuan Hong,Baozhen Ke,Weiya Xia,Yongkun Wei,Zhengyu Zha,Yan Wang,Han-Pin Kuo,Chih-Wei Wang,Chih-Yen Tu,Chia-Hung Chen,Wei-Chien Huang,Shu-Fen Chiang,Lei Nie,Junwei Hou,Chun-Te Chen,Longfei Huo,Wen-Hao Yang,Rong Deng,Katsuya Nakai,Yi-Hsin Hsu,Shih-Shin Chang,Tai-Jan Chiu,Jun Tang,Ran Zhang,Li Wang,Bingliang Fang,Ting Chen,Kwok-Kin Wong,Jennifer L Hsu,Mien-Chie Hung

Molecular carcinogenesis 58:185-195 PubMed30346064

2018

Inhibition of skin tumor promotion by TPA using a combination of topically applied ursolic acid and curcumin.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Tremmel,Okkyung Rho,Thomas J Slaga,John DiGiovanni

International journal of clinical and experimental pathology 11:2488-2496 PubMed31938361

2018

Effects of melatonin on diabetic nephropathy rats via Wnt/β-catenin signaling pathway and TGF-β-Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weichao Wang,Jie Zhang,Xiaohang Wang,Hong Wang,Qiaohua Ren,Yukun Li

The Journal of clinical investigation 125:4529-43 PubMed26571401

2015

PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Wei Liao,Jung-Mao Hsu,Weiya Xia,Hung-Ling Wang,Ying-Nai Wang,Wei-Chao Chang,Stefan T Arold,Chao-Kai Chou,Pei-Hsiang Tsou,Hirohito Yamaguchi,Yueh-Fu Fang,Hong-Jen Lee,Heng-Huan Lee,Shyh-Kuan Tai,Mhu-Hwa Yang,Maria P Morelli,Malabika Sen,John E Ladbury,Chung-Hsuan Chen,Jennifer R Grandis,Scott Kopetz,Mien-Chie Hung

Journal of neurochemistry 128:878-89 PubMed24188029

2013

HB-EGF affects astrocyte morphology, proliferation, differentiation, and the expression of intermediate filament proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Till B Puschmann,Carl Zandén,Isabell Lebkuechner,Camille Philippot,Yolanda de Pablo,Johan Liu,Milos Pekny

PloS one 7:e33846 PubMed22457794

2012

MUC1 contributes to BPDE-induced human bronchial epithelial cell transformation through facilitating EGFR activation.

Applications

WB

Species

Human

Xiuling Xu,Lang Bai,Wenshu Chen,Mabel T Padilla,Yushi Liu,Kwang Chul Kim,Steven A Belinsky,Yong Lin

The Journal of biological chemistry 286:21164-72 PubMed21525012

2011

Epidermal growth factor receptor-mediated tissue transglutaminase overexpression couples acquired tumor necrosis factor-related apoptosis-inducing ligand resistance and migration through c-FLIP and MMP-9 proteins in lung cancer cells.

Applications

WB

Species

Human

Zi Li,Xiuling Xu,Lang Bai,Wenshu Chen,Yong Lin
View all publications
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