Rabbit Recombinant Monoclonal EGFR phospho Y1173 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 35 publications.
Images
![Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (AB32578), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-y1173-antibody-e124-ab32578--western-blot-img170010.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (AB32578)")
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] (AB32578), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-y1173-antibody-e124-ab32578--immunocytochemistry-immunofluorescence-img168603.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] (AB32578)")
![Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] (AB32578), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-y1173-antibody-e124-ab32578--flow-cytometry-intracellular-img25609.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] (AB32578)")
![Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (AB32578), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-y1173-antibody-e124-ab32578--western-blot-img442726.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (AB32578)")
Publications
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- Molecular cancer therapeutics 22:667-6782023Loss of LGR5 through Therapy-induced Downregulation or Gene Ablation Is Associated with Resistance and Enhanced MET-STAT3 Signaling in Colorectal Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTressie A Posey,Joan Jacob,Ashlyn Parkhurst,Shraddha Subramanian,Liezl E Francisco,Zhengdong Liang,Kendra S CarmonPubMed 36921315
- Cancer cell 40:754-767.e62022Poziotinib for EGFR exon 20-mutant NSCLC: Clinical efficacy, resistance mechanisms, and impact of insertion location on drug sensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasir Y Elamin,Jacqulyne P Robichaux,Brett W Carter,Mehmet Altan,Hai Tran,Don L Gibbons,Simon Heeke,Frank V Fossella,Vincent K Lam,Xiuning Le,Marcelo V Negrao,Monique B Nilsson,Anisha Patel,R S K Vijayan,Jason B Cross,Jianjun Zhang,Lauren A Byers,Charles Lu,Tina Cascone,Lei Feng,Rajyalakshmi Luthra,Francis A San Lucas,Geeta Mantha,Mark Routbort,George Blumenschein,Anne S Tsao,John V HeymachPubMed 35820397
- Frontiers in cardiovascular medicine 9:7971372022ADAMTS8 Promotes Cardiac Fibrosis Partly Through Activating EGFR Dependent Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYafang Zha,Yanyan Li,Zhuowang Ge,Jian Wang,Yuheng Jiao,Jiayan Zhang,Song ZhangPubMed 35224040
- Journal of cellular physiology 237:1780-17892021Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChing-Chia Cheng,Chih-Feng Lin,Yong-Chong Lin,Tai-Horng Young,Pei-Jen LouPubMed 34806177
- Pathology international 71:752-7642021Expression of astrocyte-elevated gene-1 indicates prognostic value of fluoropyrimidine-based adjuvant chemotherapy in resectable stage III colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong-Wei Lin,Peng-Sheng Lai,Ying-Yin Chen,Chung-Yu ChenPubMed 34528330
- Experimental and therapeutic medicine 21:6382021Gallic acid suppresses colon cancer proliferation by inhibiting SRC and EGFR phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoming Lin,Guangfei Wang,Ping Liu,Lei Han,Tong Wang,Kaili Chen,Yonglin GaoPubMed 33968169
- Carbohydrate polymers 261:1179112021Cell detachment ratio on pH-responsive chitosan: A useful biometric for prognostic judgment and drug efficacy assessment in oncology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia-Hsiang Yen,Tai-Horng Young,Tsung-Wei HuangPubMed 33766385
- Neoplasia (New York, N.Y.) 23:36-482020Eucannabinolide, a novel sesquiterpene lactone, suppresses the growth, metastasis and BCSCS-like traits of TNBC via inactivation of STAT3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhihui Zhu,Jingtao Yuan,Xintong Xu,Yingying Wei,Bo Yang,Huajun ZhaoPubMed 33217668
- International journal of cancer 148:1438-14512020Integrating proteomics into precision oncology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeonie W Wahjudi,Stephan Bernhardt,Khalid Abnaof,Peter Horak,Simon Kreutzfeldt,Christoph Heining,Simone Borgoni,Corinna Becki,Daniela Berg,Daniela Richter,Barbara Hutter,Sebastian Uhrig,Katrin Pfütze,Jonas Leichsenring,Hanno Glimm,Benedikt Brors,Christof von Kalle,Albrecht Stenzinger,Ulrike Korf,Stefan Fröhling,Stefan WiemannPubMed 32949162
- Molecules (Basel, Switzerland) 25:202016-Hydroxycleroda-3,13-dien-15,16-olide Induces Apoptosis in Human Bladder Cancer Cells through Cell Cycle Arrest, Mitochondria ROS Overproduction, and Inactivation of EGFR-Related Signalling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Chi Chen et. al.PubMed 32872665
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25 - 1/50 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Recommended products
Rabbit Recombinant Monoclonal EGFR phospho Y1173 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 35 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- E124
- Purification technique
- Affinity purification Protein A
- Specificity
The antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
- Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
- Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
- Associated diseases and disorders
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-y1173-antibody-e124-ab32578--western-blot-img170010.jpg "Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)")
Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)
All lanes: Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578) at 1/1000 dilution
Lane 1: Untreated A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2: A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate
Lane 3: A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 134 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-y1173-antibody-e124-ab32578--immunocytochemistry-immunofluorescence-img168603.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)")
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)
Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with EGF(100ng/ml 5min) cells labeling EGFR with Purified ab32578 at 1:2000 dilution (0.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-y1173-antibody-e124-ab32578--flow-cytometry-intracellular-img25609.jpg "Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)")
Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade ab177178 at 1/1300 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
![Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578), expandable thumbnail](https://content.abcam.com/products/images/egfr-phospho-y1173-antibody-e124-ab32578--western-blot-img442726.jpg "Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (ab32578)
EGFR (phospho Y1173) western blot using anti-EGFR (phospho Y1173) antibody [E124] ab32578. Publication image and figure legend from Bönisch, M., Sellmann, C., et al., 2017, Protein Eng Des Sel, PubMed 28981885.
ab32578 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab32578 please see the product overview.
Biophysical and functional characterisation of B10v5 × hu225M with or without mutations in the CH1:CL interface. Analytical SEC after protein A purification of bsAbs produced in (A) HEK293-6E or (B) bsAbs produced in ExpiCHO after preparative SEC. The area under the curve in percent of all detected peak areas is given in the graph. (C) Simultaneous binding of both antigens cMET and EGFR using biolayer interferometry. BsAbs (wt, MaB40, MaB5/40, MaB21/40, MaB45/40) or antibodies consisting of only one Fab (oa for ‘one-armed’) were allowed to bind to cMET coated biosensors. Subsequently, biosensors were incubated with EGFR. (D) Differential scanning calorimetry of wildtype, interface mutants and ‘one-armed’ constructs (Table III). (E) Western blot analysis of the inhibition of EGFR and cMET phosphorylation in A549 cells by bsAbs with our without interface designs.
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