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AB203559

Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free

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(9 Publications)

Rabbit Recombinant Monoclonal EGFR phospho Y1173 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

View Alternative Names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (AB203559)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (AB203559)

Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with EGF(100ng/ml 5min) cells labeling EGFR with Purified ab32578 at 1 : 2000 dilution (0.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using ab32578, the same antibody clone in a different buffer formulation.

Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (AB203559)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (AB203559)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab177178 at 1/1300 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32578).

Western blot - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (AB203559)
  • WB

Supplier Data

Western blot - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (AB203559)

This data was developed using ab32578, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EGFR (phospho Y1173) antibody [E124] (<a href='/en-us/products/primary-antibodies/egfr-phospho-y1173-antibody-e124-ab32578'>ab32578</a>) at 1/1000 dilution

Lane 1:

Untreated A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate at 15 µg

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 134 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E124

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor).

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Reactivity data

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Product details

ab203559 is the carrier-free version of ab32578.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.
See full target information EGFR phospho Y1173
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Endocrine-related cancer 21:555-65 PubMed24825747

2014

Regulation of estrogen receptor α function in oral squamous cell carcinoma cells by FAK signaling.

Applications

Unspecified application

Species

Human

Yi-Lin Chang,Yu-Kan Hsu,Tsung-Fan Wu,Chieh-Ming Huang,Li-Yin Liou,Ya-Wen Chiu,Yu-Hsuan Hsiao,Fuh-Jinn Luo,Ta-Chun Yuan

Clinical cancer research : an official journal of the American Association for Cancer Research 19:1126-38 PubMed23209031

2012

GA201 (RG7160): a novel, humanized, glycoengineered anti-EGFR antibody with enhanced ADCC and superior in vivo efficacy compared with cetuximab.

Applications

Unspecified application

Species

Unspecified reactive species

Christian A Gerdes,Valeria Gonzalez Nicolini,Sylvia Herter,Erwin van Puijenbroek,Sabine Lang,Michaela Roemmele,Ekkehard Moessner,Olivier Freytag,Thomas Friess,Carola H Ries,Birgit Bossenmaier,Hans Joachim Mueller,Pablo Umaña

Toxicological sciences : an official journal of th 128:365-76 PubMed22552774

2012

Phthalates stimulate the epithelial to mesenchymal transition through an HDAC6-dependent mechanism in human breast epithelial stem cells.

Applications

Unspecified application

Species

Human

Tsung-Hua Hsieh,Cheng-Fang Tsai,Chia-Yi Hsu,Po-Lin Kuo,Jau-Nan Lee,Chee-Yin Chai,Ming-Feng Hou,Chia-Cheng Chang,Cheng-Yu Long,Ying-Chin Ko,Eing-Mei Tsai

Science translational medicine 4:120ra17 PubMed22277784

2012

Mechanisms of acquired crizotinib resistance in ALK-rearranged lung Cancers.

Applications

WB

Species

Human

Ryohei Katayama,Alice T Shaw,Tahsin M Khan,Mari Mino-Kenudson,Benjamin J Solomon,Balazs Halmos,Nicholas A Jessop,John C Wain,Alan Tien Yeo,Cyril Benes,Lisa Drew,Jamal Carlos Saeh,Katherine Crosby,Lecia V Sequist,A John Iafrate,Jeffrey A Engelman

Breast cancer research : BCR 13:R121 PubMed22123186

2011

Different mechanisms for resistance to trastuzumab versus lapatinib in HER2-positive breast cancers--role of estrogen receptor and HER2 reactivation.

Applications

WB

Species

Unspecified reactive species

Yen-Chao Wang,Gladys Morrison,Ryan Gillihan,Jun Guo,Robin M Ward,Xiaoyong Fu,Maria F Botero,Nuala A Healy,Susan G Hilsenbeck,Gail Lewis Phillips,Gary C Chamness,Mothaffar F Rimawi,C Kent Osborne,Rachel Schiff

Histopathology 59:376-89 PubMed22034878

2011

Epidermal growth factor receptor signalling contributes to osteoblastic stromal cell proliferation, osteoclastogenesis and disease progression in giant cell tumour of bone.

Applications

IHC

Species

Human

Peter Balla,Linda Moskovszky,Zoltan Sapi,Ramses Forsyth,Helen Knowles,Nick A Athanasou,Miklos Szendroi,Laszlo Kopper,Hajnalka Rajnai,Ferenc Pinter,Istvan Petak,Maria Serena Benassi,Piero Picci,Amalia Conti,Tibor Krenacs

Oncogene 28:461-8 PubMed18978815

2008

Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab.

Applications

Unspecified application

Species

Unspecified reactive species

M Pickl,C H Ries

Molecular pharmacology 73:1668-78 PubMed18349105

2008

Multipathway model enables prediction of kinase inhibitor cross-talk effects on migration of Her2-overexpressing mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Neil Kumar,Raffi Afeyan,Hyung-Do Kim,Douglas A Lauffenburger

Molecular therapy : the journal of the American Society of Gene Therapy 15:279-86 PubMed17235305

2007

Oncolytic HSV and erlotinib inhibit tumor growth and angiogenesis in a novel malignant peripheral nerve sheath tumor xenograft model.

Applications

WB

Species

Unspecified reactive species

Yonatan Y Mahller,Sachin S Vaikunth,Mark A Currier,Shyra J Miller,Maria C Ripberger,Ya-Hsuan Hsu,Ruty Mehrian-Shai,Margaret H Collins,Timothy M Crombleholme,Nancy Ratner,Timothy P Cripe
View all publications
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