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AB63943

Anti-EGR2 antibody

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(1 Review)

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(4 Publications )

Goat Polyclonal EGR2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EGR2 aa 200 to C-terminus.
1 Images
Western blot - Anti-EGR2 antibody (AB63943)
  • WB

Supplier Data

Western blot - Anti-EGR2 antibody (AB63943)

Incubated with the primary antibody for 1 hour.

All lanes:

Western blot - Anti-EGR2 antibody (ab63943) at 2 µg/mL

All lanes:

HepG2 cell lysate in RIPA buffer at 35 µg

Predicted band size: 50 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human EGR2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11161

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-6 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "3-5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab63943 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGR2 also known as early growth response 2 or KROX20 refers to a transcription factor with a mass of approximately 53 kDa. This protein plays a significant role in gene expression and regulates various genes involved in cellular growth and differentiation. EGR2 is expressed in various tissues with notable presence in the nervous system particularly within Schwann cells and the developing vertebrate nervous system. It can also be found in immune system cells including T and B cells.
Biological function summary

EGR2 influences the development and function of the peripheral nervous system contributing to processes like nerve myelination. It acts as a transcriptional regulator controlling the expression of genes that are essential for the maintenance of myelin sheaths around nerves. While EGR2 does not directly form part of a protein complex its role as a transcription factor allows it to interact with many other proteins to exert its regulatory functions.

Pathways

EGR2 participates in cellular pathways such as the MAPK/ERK signaling pathway and the immune response pathway. In the MAPK/ERK pathway EGR2 interacts with proteins like ERK1/2 to regulate cell growth and differentiation. In immune response pathways EGR2 modulates the differentiation of T cells impacting immune homeostasis and function by influencing gene expression pertinent to immune regulation.

Mutations or dysregulation of EGR2 can relate to Charcot-Marie-Tooth disease type 1 a peripheral nerve disorder. This neuropathy impacts myelin production where alterations in EGR2 play a role alongside proteins like PMP22 which are also affected in the disorder. Additionally EGR2 expression irregularities have been studied in connection with systemic lupus erythematosus in which immune cell regulation becomes impaired affecting proteins involved in immune signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific DNA-binding transcription factor (PubMed : 17717711). Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin (By similarity). Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation (By similarity). Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2 (By similarity). Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres (By similarity). Promotes the expression of HOXB3 in the rhombomere r5 in the hindbrain (By similarity). Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells (By similarity). Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons (By similarity). May play a role in adipogenesis, possibly by regulating the expression of CEBPB (By similarity).. E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity.
See full target information EGR2

Alternative Names

KROX20, EGR2, E3 SUMO-protein ligase EGR2, AT591, E3 SUMO-protein transferase ERG2, Early growth response protein 2, Zinc finger protein Krox-20, EGR-2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:110355 PubMed40505865

2025

Select early growth response (Egr) isoforms augment hypoxia inducible factor 2 (HIF-2) regulation of erythropoietin (Epo) gene expression in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Jason S Nagati,Elhadji M Dioum,Catarina S Giardinetto,S M Golam Mohiuddin,Qiuyang Zhang,Joseph A Garcia

The Journal of clinical investigation 130:4546-4560 PubMed32484801

2020

FTY720 reactivates cryptococcal granulomas in mice through S1P receptor 3 on macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Arielle M Bryan,Jeehyun Karen You,Travis McQuiston,Cristina Lazzarini,Zhijuan Qiu,Brian Sheridan,Barbara Nuesslein-Hildesheim,Maurizio Del Poeta

Acta neuropathologica communications 8:51 PubMed32303273

2020

Characterising cellular and molecular features of human peripheral nerve degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew B Wilcox,Simão G Laranjeira,Tuula M Eriksson,Kristjan R Jessen,Rhona Mirsky,Tom J Quick,James B Phillips

Cells 8: PubMed31783583

2019

Advanced Age Is Associated with Iron Dyshomeostasis and Mitochondrial DNA Damage in Human Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Picca,Robert T Mankowski,George Kamenov,Stephen D Anton,Todd M Manini,Thomas W Buford,Sunil K Saini,Riccardo Calvani,Francesco Landi,Roberto Bernabei,Emanuele Marzetti,Christiaan Leeuwenburgh
View all publications
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Product promise

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