Rabbit Polyclonal EGR4 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Early growth response protein 4.
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Publications
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- JCI insight 6:2021The DNA repair protein ATM as a target in autism spectrum disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLara Pizzamiglio et. al.PubMed 33373327
- Biochimie 149:62-702018Overexpression of Egr2 and Egr4 protects rat brains against ischemic stroke by downregulating JNK signaling pathway.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Early growth response protein 4. The exact immunogen used to generate this antibody is proprietary information. Database link Q05215
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/25.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation (By similarity).
Alternative names
Early growth response protein 4, EGR-4, AT133, EGR4
Recommended products
Rabbit Polyclonal EGR4 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Early growth response protein 4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Early growth response protein 4. The exact immunogen used to generate this antibody is proprietary information. Database link Q05215
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
{EGR4} also known as Early Growth Response 4 is a transcription factor involved in the regulation of gene expression. It belongs to the EGR family of C2H2-type zinc-finger proteins. EGR4 has a mass of approximately 59 kDa and expresses in a variety of tissues including the brain testis and other nervous tissues. The protein serves as an important regulator in neuronal differentiation and development playing a role in synaptic plasticity and cognitive functions.
- Biological function summary
The Early Growth Response 4 protein modulates the transcription of genes involved in cellular growth and apoptosis. It does not work in large complexes but often collaborates with other transcription factors and cofactors. This interaction helps control the expression of genes necessary for cell proliferation differentiation and stress response. EGR4’s activity significantly affects the balance between cell survival and death impacting cellular homeostasis and adaptation to external signals.
- Pathways
EGR4 interacts with multiple signal transduction pathways. Notably it engages in the MAPK/ERK pathway which plays a critical role in transmitting growth signals from the cell surface to the nucleus. Moreover EGR4 influences the PI3K/AKT pathway related to cell survival and metabolism. These pathways connect EGR4 with other proteins such as c-JUN and CREB important for gene regulation and cellular response mechanisms.
- Associated diseases and disorders
The dysregulation of EGR4 has been associated with certain neurological disorders and cancers. In particular aberrant expression of EGR4 links to Alzheimer's disease where it might contribute to neuronal damage and impaired cognitive functions. Additionally EGR4 involvement appears in the progression of testicular tumors indicating its role in cell proliferation and malignancy. EGR4's interaction with p53 a prominent tumor suppressor protein could exacerbate or mitigate these disease processes by influencing apoptosis or cell cycle regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-EGR4 antibody (ab198197)
8% SDS-PAGE
All lanes: Western blot - Anti-EGR4 antibody (ab198197) at 1/400 dilution
All lanes: Human fetal brain tissue lysate at 40 µg
Predicted band size: 51 kDa
Exposure time: 35s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGR4 antibody (ab198197)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling EGR4 with ab198197 at a 1/25 dilution.
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