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AB122557

Anti-EHBP1L1 antibody

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(2 Publications)

Rabbit Polyclonal EHBP1L1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EHBP1L1.

View Alternative Names

EH domain-binding protein 1-like protein 1, EHBP1L1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (AB122557)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (AB122557)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lymph node tissue labeling EHBP1L1 with ab122557 at 1/5000.

Immunocytochemistry/ Immunofluorescence - Anti-EHBP1L1 antibody (AB122557)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EHBP1L1 antibody (AB122557)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122557 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (AB122557)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (AB122557)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling EHBP1L1 with ab122557 at 1/5000.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (AB122557)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (AB122557)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skeletal muscle tissue labeling EHBP1L1 with ab122557 at 1/5000.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (AB122557)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (AB122557)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebral cortex tissue labeling EHBP1L1 with ab122557 at 1/5000. No positivity observed.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EHBP1L1. The exact immunogen used to generate this antibody is proprietary information.

Q8N3D4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EHBP1L1 protein also known as EH domain binding protein 1-like 1 is involved in endocytic trafficking processes. It has a molecular mass of approximately 98 kDa. This protein is expressed in various tissues particularly showing higher expression levels in the epithelial cells of the gastrointestinal tract and kidney. EHBP1L1 contains multiple EH domains which help mediate protein-protein interactions necessary for cellular transport processes.
Biological function summary

EHBP1L1 facilitates the movement of vesicles by linking motor proteins to cell membranes. It participates in cellular processes such as cytoskeletal organization and membrane remodeling through its interaction with other components in a protein complex. This involvement ensures proper cellular function and maintenance of dynamic cellular structures.

Pathways

EHBP1L1 plays a role in the endocytic recycling pathway. It is essential for the internalization and recycling of membrane-bound receptors. EHBP1L1 interacts with proteins such as Rab8 and Rab11 which are small GTPases critical for membrane traffic. This protein's role helps maintain cellular homeostasis and signaling by facilitating receptor recycling.

EHBP1L1 has been linked to certain renal disorders particularly ones affecting epithelial cell function. Its association with proteins like Rab8 indicates a potential role in cystic kidney disease where vesicle trafficking pathways are disrupted. Additionally abnormal EHBP1L1 expression has connections to gastrointestinal cancers. These disorders highlight the importance of maintaining proper vesicle trafficking and membrane dynamics in cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as Rab effector protein and play a role in vesicle trafficking.
See full target information EHBP1L1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012344 PubMed38976714

2024

Genome-wide CRISPR screenings identified SMCHD1 as a host-restricting factor for AAV transduction.

Applications

Unspecified application

Species

Unspecified reactive species

Chenlu Wang,Yu Liu,Jingfei Xiong,Kun Xie,Tianshu Wang,Yu Hu,Huancheng Fu,Baiquan Zhang,Xiaochao Huang,Hui Bao,Haoyang Cai,Biao Dong,Zhonghan Li

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2206792 PubMed36775874

2023

EHBP1L1 Drives Immune Evasion in Renal Cell Carcinoma through Binding and Stabilizing JAK1.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Pan,Guannan Shu,Liangmin Fu,Kangbo Huang,Xinwei Zhou,Chengpeng Gui,Huashan Liu,Xiaohan Jin,Minyu Chen,Pengju Li,Junjie Cen,Zihao Feng,Jun Lu,Zhenhua Chen,Jiaying Li,Quanhui Xu,Yinghan Wang,Hui Liang,Zhu Wang,Qiong Deng,Wei Chen,Junhang Luo,Jiefeng Yang,Jiaxing Zhang,Jinhuan Wei
View all publications
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