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AB109747

Anti-EHD1 antibody [EPR4955]

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(7 Publications)

Rabbit Recombinant Monoclonal EHD1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

View Alternative Names

PAST, PAST1, CDABP0131, EHD1, EH domain-containing protein 1, PAST homolog 1, Testilin, hPAST1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EHD1 antibody [EPR4955] (AB109747)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EHD1 antibody [EPR4955] (AB109747)

ab109747, at 1/100 dilution, staining EHD1 in HeLa cells by Immunofluorescence.

Flow Cytometry (Intracellular) - Anti-EHD1 antibody [EPR4955] (AB109747)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-EHD1 antibody [EPR4955] (AB109747)

Overlay histogram showing HeLa cells stained with ab109747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109747 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Western blot - Anti-EHD1 antibody [EPR4955] (AB109747)
  • WB

Unknown

Western blot - Anti-EHD1 antibody [EPR4955] (AB109747)

All lanes:

Western blot - Anti-EHD1 antibody [EPR4955] (ab109747) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Predicted band size: 61 kDa

false

  • Carrier free

    Anti-EHD1 antibody [EPR4955] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4955

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 66% homology with EHD3. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000 - 1/10000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHD1 also known as Eps15 homology domain-containing protein 1 or EH domain-containing protein 1 is an important component in the process of endocytic recycling. EHD1 belongs to the C-terminal EHD protein family and it has a molecular mass of approximately 61 kDa. Expression of EHD1 occurs in various tissues with significant levels in the brain heart and skeletal muscle. As a member of this family EHD1 plays an authoritative role in membrane trafficking specifically in the cycling of endosomes back to the plasma membrane.
Biological function summary

In cellular transport and organization EHD1 regulates the recycling and sorting of endocytic vesicles. It functions in concert with other partners including Rab proteins and syndapin forming a complex that promotes the tubulation and fission of recycling carriers. This involvement is fundamental for maintaining cell surface receptor levels and proper cell signaling. EHD1's role is necessary to ensure cellular homeostasis and receptor-mediated processes.

Pathways

EHD1 plays an influential role in intracellular trafficking pathways particularly the endocytic recycling pathway and the trafficking of GLUT4 in adipocytes and muscle cells. In collaboration with proteins such as Rab11 and SNX5 it supports the movement of recycled membrane proteins and receptors back to the cell surface. In the GLUT4 pathway EHD1 assists in glucose uptake by facilitating the correct localization of glucose transporters on the plasma membrane which is critical for maintaining glucose homeostasis.

EHD1's dysfunction has been linked to neurodegenerative diseases and cancer. Abnormal EHD1 activity can impact neuronal signaling pathways contributing to conditions such as Alzheimer's disease. The alteration of its function can also promote disrupted recycling of growth factor receptors therefore potentially contributing to oncogenic processes. EHD1’s interactions with proteins like Rab11 and SNX5 in these contexts highlight its involvement in maintaining cellular integrity and normal function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes (PubMed : 24019528). Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes (PubMed : 15020713, PubMed : 17233914, PubMed : 20801876). Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity). Plays a role in myoblast fusion (By similarity). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis (PubMed : 25686250).
See full target information EHD1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 79:615 PubMed36460928

2022

The endocytic recycling compartment serves as a viral factory for hepatitis E virus.

Applications

Unspecified application

Species

Unspecified reactive species

Cyrine Bentaleb,Kévin Hervouet,Claire Montpellier,Charline Camuzet,Martin Ferrié,Julien Burlaud-Gaillard,Stéphane Bressanelli,Karoline Metzger,Elisabeth Werkmeister,Maliki Ankavay,Nancy Leon Janampa,Julien Marlet,Julien Roux,Clarence Deffaud,Anne Goffard,Yves Rouillé,Jean Dubuisson,Philippe Roingeard,Cécile-Marie Aliouat-Denis,Laurence Cocquerel

Human molecular genetics 30:1851-1862 PubMed34100076

2021

Functional and behavioral effects of de novo mutations in calcium-related genes in patients with bipolar disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Takumi Nakamura,Kazuo Nakajima,Yuki Kobayashi,Shigeyoshi Itohara,Takaoki Kasahara,Takashi Tsuboi,Tadafumi Kato

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33653948

2021

Pathogenic LRRK2 regulates ciliation probability upstream of tau tubulin kinase 2 via Rab10 and RILPL1 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yuriko Sobu,Paulina S Wawro,Herschel S Dhekne,Wondwossen M Yeshaw,Suzanne R Pfeffer

Nature communications 8:1246 PubMed29093443

2017

Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuyuki Matsudaira,Kojiro Mukai,Taishin Noguchi,Junya Hasegawa,Tomohisa Hatta,Shun-Ichiro Iemura,Tohru Natsume,Norio Miyamura,Hiroshi Nishina,Jun Nakayama,Kentaro Semba,Takuya Tomita,Shigeo Murata,Hiroyuki Arai,Tomohiko Taguchi

Journal of cell science 130:1147-1157 PubMed28137756

2017

Endocytic turnover of Rab8 controls cell polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Maite Vidal-Quadras,Mikkel R Holst,Monika K Francis,Elin Larsson,Mariam Hachimi,Wai-Lok Yau,Johan Peränen,Fernando Martín-Belmonte,Richard Lundmark

Cilia 5:23 PubMed27493724

2016

The IN/OUT assay: a new tool to study ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ira Kukic,Felix Rivera-Molina,Derek Toomre

Developmental biology 393:298-311 PubMed25019370

2014

GRAF1 promotes ferlin-dependent myoblast fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlin C Lenhart,Abby L Becherer,Jianbin Li,Xiao Xiao,Elizabeth M McNally,Christopher P Mack,Joan M Taylor
View all publications
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