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AB247922

Anti-EHD1 antibody [EPR4955] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EHD1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PAST, PAST1, CDABP0131, EHD1, EH domain-containing protein 1, PAST homolog 1, Testilin, hPAST1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EHD1 antibody [EPR4955] - BSA and Azide free (AB247922)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EHD1 antibody [EPR4955] - BSA and Azide free (AB247922)

This data was developed using ab109747, the same antibody clone in a different buffer formulation.ab109747, at 1/100 dilution, staining EHD1 in HeLa cells by Immunofluorescence.

Flow Cytometry (Intracellular) - Anti-EHD1 antibody [EPR4955] - BSA and Azide free (AB247922)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-EHD1 antibody [EPR4955] - BSA and Azide free (AB247922)

This data was developed using ab109747, the same antibody clone in a different buffer formulation.

Overlay histogram showing HeLa cells stained with ab109747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109747, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Western blot - Anti-EHD1 antibody [EPR4955] - BSA and Azide free (AB247922)
  • WB

Unknown

Western blot - Anti-EHD1 antibody [EPR4955] - BSA and Azide free (AB247922)

This data was developed using ab109747, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EHD1 antibody [EPR4955] (<a href='/en-us/products/primary-antibodies/ehd1-antibody-epr4955-ab109747'>ab109747</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Predicted band size: 61 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4955

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 66% homology with EHD3. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab247922 is the carrier-free version of ab109747.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHD1 also known as Eps15 homology domain-containing protein 1 or EH domain-containing protein 1 is an important component in the process of endocytic recycling. EHD1 belongs to the C-terminal EHD protein family and it has a molecular mass of approximately 61 kDa. Expression of EHD1 occurs in various tissues with significant levels in the brain heart and skeletal muscle. As a member of this family EHD1 plays an authoritative role in membrane trafficking specifically in the cycling of endosomes back to the plasma membrane.
Biological function summary

In cellular transport and organization EHD1 regulates the recycling and sorting of endocytic vesicles. It functions in concert with other partners including Rab proteins and syndapin forming a complex that promotes the tubulation and fission of recycling carriers. This involvement is fundamental for maintaining cell surface receptor levels and proper cell signaling. EHD1's role is necessary to ensure cellular homeostasis and receptor-mediated processes.

Pathways

EHD1 plays an influential role in intracellular trafficking pathways particularly the endocytic recycling pathway and the trafficking of GLUT4 in adipocytes and muscle cells. In collaboration with proteins such as Rab11 and SNX5 it supports the movement of recycled membrane proteins and receptors back to the cell surface. In the GLUT4 pathway EHD1 assists in glucose uptake by facilitating the correct localization of glucose transporters on the plasma membrane which is critical for maintaining glucose homeostasis.

EHD1's dysfunction has been linked to neurodegenerative diseases and cancer. Abnormal EHD1 activity can impact neuronal signaling pathways contributing to conditions such as Alzheimer's disease. The alteration of its function can also promote disrupted recycling of growth factor receptors therefore potentially contributing to oncogenic processes. EHD1’s interactions with proteins like Rab11 and SNX5 in these contexts highlight its involvement in maintaining cellular integrity and normal function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes (PubMed : 24019528). Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes (PubMed : 15020713, PubMed : 17233914, PubMed : 20801876). Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity). Plays a role in myoblast fusion (By similarity). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis (PubMed : 25686250).
See full target information EHD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of investigative dermatology 136:2003-2012 PubMed27349864

2016

Thyroid Hormones Enhance Mitochondrial Function in Human Epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Vidali,Jérémy Chéret,Melanie Giesen,Swantje Haeger,Majid Alam,Rachel E B Watson,Abigail K Langton,Matthias Klinger,Jana Knuever,Wolfgang Funk,Barbara Kofler,Ralf Paus
View all publications
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Product promise

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