AB222888

Anti-EHD2 antibody

Name: Anti-EHD2 antibody (ab222888) | Abcam
Brand: Abcam Limited
SKU: AB222888
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal EHD2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EHD2 aa 400 to C-terminus.

View Alternative Names

PAST2, EHD2, EH domain-containing protein 2, PAST homolog 2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody (AB222888)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody (AB222888)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EHD2 (green) using ab222888 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD2 antibody (AB222888)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD2 antibody (AB222888)

Paraffin-embedded human kidney tissue stained for EHD2 using ab222888 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-EHD2 antibody (AB222888)

All lanes:

Western blot - Anti-EHD2 antibody (ab222888) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Mouse liver lysate

Lane 3:

Mouse kidney lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 61 kDa

Observed band size: 62 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human EHD2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NZN4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHD2 also known as EH domain-containing protein 2 is a mechanochemical protein with a molecular mass of approximately 61 kDa. It belongs to the dynamin superfamily of GTPases. EHD2 is frequently expressed in muscle cells adipocytes and cardiac tissues. It associates with cellular membranes particularly near caveolae where it plays a pivotal role in regulating cellular membrane dynamics and endocytosis through binding to phosphoinositides.

Biological function summary

The protein connection with membranes affects the regulation of vesicle trafficking and endosomal recycling. EHD2 functions as part of a complex with other proteins like pacsin and syndapin which modulates curvature of membranes a process essential for diverse cellular activities. By facilitating endocytosis EHD2 has a role in signaling pathways that influence cell migration and membrane repair.

Pathways

EHD2 interacts significantly with lipid raft-associated pathways which are critical in cellular communication and signal transduction. This protein interacts with the caveolin protein family particularly Caveolin-1 during caveolae-mediated endocytosis. It contributes to the spatial organization of signaling domains impacting the cellular response to external stimuli and stress.

The malfunction or altered expression of EHD2 associates with disorders such as obesity and muscle-related diseases. EHD2 dysregulation can affect Caveolin-1 contributing to abnormal vesicular transport and lipid metabolism. Such dysfunction holds potential links to the development or progression of conditions like cardiac hypertrophy highlighting the protein's importance in cellular health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity). Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed : 17233914). Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (PubMed : 25588833).

See full target information EHD2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 64:15 PubMed38095908

2023

JAM-C Is Important for Lens Epithelial Cell Proliferation and Lens Fiber Maturation in Murine Lens Development.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Sun,Jiani Li,Jingyu Ma,Yuxing Zheng,Rong Ju,Xuri Li,Xiangrong Ren,Lijuan Huang,Rongyuan Chen,Xuhua Tan,Lixia Luo

International journal of clinical and experimental pathology 16:344-351 PubMed38059172

2023

EHD2, a novel HIF target gene, is a promising biomarker in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Chen,Song Xue,Jian Shi,Chunfeng He,Qingchuan Zhang

Nature communications 13:7234 PubMed36433988

2022

The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Matthaeus,Kem A Sochacki,Andrea M Dickey,Dmytro Puchkov,Volker Haucke,Martin Lehmann,Justin W Taraska

Journal of thoracic disease 14:2652-2664 PubMed35928621

2022

inhibits the invasive ability of lung adenocarcinoma and improves the prognosis of patients.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Wei,Jingjing Shao,Jinming Wang,Tianye Zhao,Jia Liu,Xiying Shen,Yidan Wang,Haizhen Chen,Gaoren Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EHD2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody (AB222888)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD2 antibody (AB222888)

Western blot - Anti-EHD2 antibody (AB222888)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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