AB154784

Anti-EHD2 antibody [EPR9821]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal EHD2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

PAST2, EHD2, EH domain-containing protein 2, PAST homolog 2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody [EPR9821] (AB154784)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody [EPR9821] (AB154784)

Immunofluorescent analysis of HeLa cells labeling EHD2 with ab154784 at 1/250 dilution.

Unknown

Western blot - Anti-EHD2 antibody [EPR9821] (AB154784)

All lanes:

Western blot - Anti-EHD2 antibody [EPR9821] (ab154784) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 61 kDa

false

Carrier free

Anti-EHD2 antibody [EPR9821] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9821

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHD2 also known as EH domain-containing protein 2 is a mechanochemical protein with a molecular mass of approximately 61 kDa. It belongs to the dynamin superfamily of GTPases. EHD2 is frequently expressed in muscle cells adipocytes and cardiac tissues. It associates with cellular membranes particularly near caveolae where it plays a pivotal role in regulating cellular membrane dynamics and endocytosis through binding to phosphoinositides.

Biological function summary

The protein connection with membranes affects the regulation of vesicle trafficking and endosomal recycling. EHD2 functions as part of a complex with other proteins like pacsin and syndapin which modulates curvature of membranes a process essential for diverse cellular activities. By facilitating endocytosis EHD2 has a role in signaling pathways that influence cell migration and membrane repair.

Pathways

EHD2 interacts significantly with lipid raft-associated pathways which are critical in cellular communication and signal transduction. This protein interacts with the caveolin protein family particularly Caveolin-1 during caveolae-mediated endocytosis. It contributes to the spatial organization of signaling domains impacting the cellular response to external stimuli and stress.

The malfunction or altered expression of EHD2 associates with disorders such as obesity and muscle-related diseases. EHD2 dysregulation can affect Caveolin-1 contributing to abnormal vesicular transport and lipid metabolism. Such dysfunction holds potential links to the development or progression of conditions like cardiac hypertrophy highlighting the protein's importance in cellular health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity). Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed : 17233914). Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (PubMed : 25588833).

See full target information EHD2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 34:ar124 PubMed37703099

2023

EHD2 regulates plasma membrane integrity and downstream insulin receptor signaling events.

Applications

Unspecified application

Species

Unspecified reactive species

Mathis Neuhaus,Claes Fryklund,Holly Taylor,Andrea Borreguero-Muñoz,Franziska Kopietz,Hamidreza Ardalani,Oksana Rogova,Laura Stirrat,Shaun K Bremner,Peter Spégel,Nia J Bryant,Gwyn W Gould,Karin G Stenkula

Nature communications 14:4557 PubMed37507371

2023

Cancer cell-mitochondria hybrid membrane coated Gboxin loaded nanomedicines for glioblastoma treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zou,Yajing Sun,Yibin Wang,Dongya Zhang,Huiqing Yang,Xin Wang,Meng Zheng,Bingyang Shi

Molecular biology of the cell 30:1147-1159 PubMed30811273

2019

EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Björn Morén,Björn Hansson,Florentina Negoita,Claes Fryklund,Richard Lundmark,Olga Göransson,Karin G Stenkula

Science advances 2:e1601470 PubMed28028537

2016

A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Li,Ryan J Schulze,Shaun G Weller,Eugene W Krueger,Micah B Schott,Xiaodong Zhang,Carol A Casey,Jun Liu,Jacqueline Stöckli,David E James,Mark A McNiven

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EHD2 antibody [EPR9821]

Immunocytochemistry/ Immunofluorescence - Anti-EHD2 antibody [EPR9821] (AB154784)

Western blot - Anti-EHD2 antibody [EPR9821] (AB154784)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise