AB151694

Anti-EHD4 antibody [EPR9816(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal EHD4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

HCA10, HCA11, PAST4, FKSG7, EHD4, EH domain-containing protein 4, Hepatocellular carcinoma-associated protein 10/11, PAST homolog 4

1 Images

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Western blot - Anti-EHD4 antibody [EPR9816(B)] (AB151694)

All lanes:

Western blot - Anti-EHD4 antibody [EPR9816(B)] (ab151694) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

BxPC3 cell lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9816(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 93% homology with EHD3 and EHD1, 79% homology with EHD2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHD4 also known as EH domain-containing protein 4 is a member of the C-terminal Eps15 homology domain-containing protein family. This protein has a mass of approximately 68 kDa. Mechanically EHD4 participates in the regulation of endocytic transport by assisting in the remodeling of membranes. It is expressed in different tissues significantly in the brain and testes where it fulfills several cellular functions.

Biological function summary

EHD4 plays a role in intracellular trafficking processes essential for cellular homeostasis. It associates with the endosomal recycling complex facilitating the transport of receptors from endosomes back to the plasma membrane or the trans-Golgi network. This activity supports the maintenance of receptor availability on the cell surface influencing cellular responsiveness to external stimuli.

Pathways

EHD4 functions within endocytic and recycling pathways coordinating these with other cellular proteins. For instance EHD4 interacts with RAB proteins such as RAB11 to regulate membrane dynamics and vesicular traffic. These interactions are critical in the coordination and regulation of proteins involved in the recycling of membrane compartments within the cell.

EHD4 has been implicated in neurodegenerative diseases and certain forms of cancer. In the context of cancer EHD4's role in membrane trafficking affects cell signaling and proliferation while its dysregulation can lead to aberrant cellular behaviors. Furthermore it interacts with other proteins like EHD1 influencing pathophysiological processes linked to these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.

See full target information EHD4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 11:2032-2043 PubMed32127931

2020

MRPS16 facilitates tumor progression via the PI3K/AKT/Snail signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Wang,Junjun Li,Xiaobing Long,Liwu Jiao,Minghui Zhou,Kang Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EHD4 antibody [EPR9816(B)]

Western blot - Anti-EHD4 antibody [EPR9816(B)] (AB151694)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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