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AB249018

Anti-EHD4 antibody [EPR9816(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EHD4 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HCA10, HCA11, PAST4, FKSG7, EHD4, EH domain-containing protein 4, Hepatocellular carcinoma-associated protein 10/11, PAST homolog 4

1 Images
Western blot - Anti-EHD4 antibody [EPR9816(B)] - BSA and Azide free (AB249018)
  • WB

Unknown

Western blot - Anti-EHD4 antibody [EPR9816(B)] - BSA and Azide free (AB249018)

This data was developed using ab151694, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EHD4 antibody [EPR9816(B)] (<a href='/en-us/products/primary-antibodies/ehd4-antibody-epr9816b-ab151694'>ab151694</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

BxPC3 cell lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 61 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9816(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 93% homology with EHD3 and EHD1, 79% homology with EHD2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

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Product details

ab249018 is the carrier-free version of ab151694.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHD4 also known as EH domain-containing protein 4 is a member of the C-terminal Eps15 homology domain-containing protein family. This protein has a mass of approximately 68 kDa. Mechanically EHD4 participates in the regulation of endocytic transport by assisting in the remodeling of membranes. It is expressed in different tissues significantly in the brain and testes where it fulfills several cellular functions.
Biological function summary

EHD4 plays a role in intracellular trafficking processes essential for cellular homeostasis. It associates with the endosomal recycling complex facilitating the transport of receptors from endosomes back to the plasma membrane or the trans-Golgi network. This activity supports the maintenance of receptor availability on the cell surface influencing cellular responsiveness to external stimuli.

Pathways

EHD4 functions within endocytic and recycling pathways coordinating these with other cellular proteins. For instance EHD4 interacts with RAB proteins such as RAB11 to regulate membrane dynamics and vesicular traffic. These interactions are critical in the coordination and regulation of proteins involved in the recycling of membrane compartments within the cell.

EHD4 has been implicated in neurodegenerative diseases and certain forms of cancer. In the context of cancer EHD4's role in membrane trafficking affects cell signaling and proliferation while its dysregulation can lead to aberrant cellular behaviors. Furthermore it interacts with other proteins like EHD1 influencing pathophysiological processes linked to these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.
See full target information EHD4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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