AB272671

Anti-EHF antibody

Name: Anti-EHF antibody (ab272671) | Abcam
Brand: Abcam Limited
SKU: AB272671
Price: 655 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal EHF antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EHF aa 1-100.

View Alternative Names

ESE3, ESE3B, ESEJ, EHF, ETS homologous factor, hEHF, ETS domain-containing transcription factor, Epithelium-specific Ets transcription factor 3, ESE-3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHF antibody (AB272671)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHF antibody (AB272671)

Paraffin-embedded formalin-fixed human salivary tissue stained for EHF using ab272671 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHF antibody (AB272671)

Paraffin-embedded formalin-fixed human breast tissue stained for EHF using ab272671 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHF antibody (AB272671)

Paraffin-embedded formalin-fixed human liver tissue stained for EHF using ab272671 at 1/200 dilution in immunohistochemical analysis. Shows no expression as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EHF aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9NZC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHF also known as Ets homologous factor is a transcription factor within the ETS family featuring a mass of approximately 37 kDa. EHF regulates gene expression by binding to DNA sequences known as ETS motifs and influences the transcriptional processes. It exhibits significant expression in epithelial tissues including the lungs and esophagus indicating its importance in cellular activities within these tissues.

Biological function summary

This protein contributes to the regulation of epithelial cell differentiation and proliferation. EHF is not generally known to be a part of specific protein complexes but works closely with other ETS family transcription factors to exert its effects on target genes. Its influence extends to controlling factors that maintain epithelial integrity and function making it important for normal tissue development and homeostasis.

Pathways

EHF impacts gene expression involved in the growth signaling and differentiation. It participates chiefly in pathways such as the MAPK/ERK pathway influencing cell cycle regulation and response to growth stimuli. EHF interacts with various MAPK proteins coordinating cellular responses that dictate epithelial cell fate decisions.

EHF correlates with conditions like cancer and inflammatory disorders. Aberrant EHF expression links to certain malignancies including lung and esophageal cancers where it may act as a tumor suppressor or promoter depending on the context. In inflammatory disorders dysregulation of EHF expression affects pathways alongside proteins such as NF-kB contributing to chronic inflammation and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.

See full target information EHF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diagnostics (Basel, Switzerland) 12: PubMed35453848

2022

The ETS Homologous Factor (EHF) Represents a Useful Immunohistochemical Marker for Predicting Prostate Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Scimeca,Manuela Montanaro,Rita Bonfiglio,Lucia Anemona,Enrico Finazzi Agrò,Anastasios D Asimakopoulos,Roberto Bei,Vittorio Manzari,Nicoletta Urbano,Erica Giacobbi,Francesca Servadei,Elena Bonanno,Orazio Schillaci,Alessandro Mauriello

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EHF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHF antibody (AB272671)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHF antibody (AB272671)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHF antibody (AB272671)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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