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Mouse Monoclonal EHHADH antibody. Suitable for ICC, IP and reacts with Human samples.

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Images

Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (AB110299), expandable thumbnail
  • Immunocytochemistry - Anti-EHHADH antibody [7F6AF11] (AB110299), expandable thumbnail

Key facts

Isotype
IgG3
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIP
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway (By similarity). Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (By similarity). Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed:15060085). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (PubMed:15060085).

Alternative names

ECHD, EHHADH, Peroxisomal bifunctional enzyme, PBE, PBFE, L-bifunctional protein, Multifunctional enzyme 1, LBP, MFE1

Recommended products

Mouse Monoclonal EHHADH antibody. Suitable for ICC, IP and reacts with Human samples.

Key facts

Isotype
IgG3
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
7F6AF11
Purification technique
Proprietary technique
Light chain type
kappa
Concentration
Loading...
Purification notes

Near homogeneity as judged by SDS-PAGE (>95% purity). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EHHADH also known as enoyl-CoA hydratase/3-hydroxyacyl CoA dehydrogenase is a mitochondrial enzyme. Its molecular mass is approximately 82 kDa. EHHADH catalyzes two sequential reactions in the β-oxidation of fatty acids converting L-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. This enzyme expresses mainly in liver and kidney tissues playing a role in lipid metabolism.

Biological function summary

EHHADH acts within the peroxisome a cellular organelle that facilitates lipid metabolism and detoxification processes. EHHADH is a part of the mitochondrial trifunctional protein complex. This complex helps in breaking down long-chain fatty acids highlighting EHHADH's role in energy production.

Pathways

EHHADH engages in the fatty acid β-oxidation pathway and the peroxisomal fatty acid β-oxidation pathway. In these pathways EHHADH works alongside proteins like HADHA and HADHB both of which are part of the trifunctional protein complex. The collaboration between these proteins ensures efficient lipid degradation and energy release.

Associated diseases and disorders

EHHADH links to disorders such as peroxisomal biogenesis disorders and Zellweger syndrome. Mutations affecting EHHADH or its associated proteins like VLCAD (very-long-chain acyl-CoA dehydrogenase) can disrupt normal mitochondrial functions. This disruption can lead to accumulation of unmetabolized fatty acids contributing to disease progression.

Product promise

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Full details and terms and conditions can be found here:
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2 product images

  • Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299), expandable thumbnail

    Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)

    Immonprecipitation analysis of enriched subcellular organelles from human liver, which contains mitochondria and peroxisome. EHHADH immunocaptured using ab110299.

    All lanes: Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)

    Predicted band size: 79 kDa

  • Immunocytochemistry - Anti-EHHADH antibody [7F6AF11] (ab110299), expandable thumbnail

    Immunocytochemistry - Anti-EHHADH antibody [7F6AF11] (ab110299)

    Immunocytochemistry image of EHHADH stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated ab110299 at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.

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Product protocols

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