AB123490

Anti-EHHADH antibody

Name: Anti-EHHADH antibody (ab123490) | Abcam
Brand: Abcam Limited
SKU: AB123490
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal EHHADH antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human EHHADH aa 650 to C-terminus.

View Alternative Names

ECHD, EHHADH, Peroxisomal bifunctional enzyme, PBE, PBFE, L-bifunctional protein, Multifunctional enzyme 1, LBP, MFE1

2 Images

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Immunoprecipitation - Anti-EHHADH antibody (AB123490)

Lane 1 : Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of EHHADH.
Lane 2 : Detection of Human EHHADH by Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123490 at 6 µg/mg lysate for IP.
Lane 3 : Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab123490 at 1 µg/ml.
Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-EHHADH antibody (ab123490)

Predicted band size: 79 kDa

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Unknown

Western blot - Anti-EHHADH antibody (AB123490)

All lanes:

Western blot - Anti-EHHADH antibody (ab123490) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human EHHADH aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q08426

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHHADH also known as enoyl-CoA hydratase/3-hydroxyacyl CoA dehydrogenase is a mitochondrial enzyme. Its molecular mass is approximately 82 kDa. EHHADH catalyzes two sequential reactions in the β-oxidation of fatty acids converting L-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. This enzyme expresses mainly in liver and kidney tissues playing a role in lipid metabolism.

Biological function summary

EHHADH acts within the peroxisome a cellular organelle that facilitates lipid metabolism and detoxification processes. EHHADH is a part of the mitochondrial trifunctional protein complex. This complex helps in breaking down long-chain fatty acids highlighting EHHADH's role in energy production.

Pathways

EHHADH engages in the fatty acid β-oxidation pathway and the peroxisomal fatty acid β-oxidation pathway. In these pathways EHHADH works alongside proteins like HADHA and HADHB both of which are part of the trifunctional protein complex. The collaboration between these proteins ensures efficient lipid degradation and energy release.

EHHADH links to disorders such as peroxisomal biogenesis disorders and Zellweger syndrome. Mutations affecting EHHADH or its associated proteins like VLCAD (very-long-chain acyl-CoA dehydrogenase) can disrupt normal mitochondrial functions. This disruption can lead to accumulation of unmetabolized fatty acids contributing to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway (By similarity). Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (By similarity). Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed : 15060085). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (PubMed : 15060085).

See full target information EHHADH

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Metabolites 15: PubMed40422873

2025

Compound 3d Attenuates Metabolic Dysfunction-Associated Steatohepatitis via Peroxisome Proliferator-Activated Receptor Pathway Activation and Inhibition of Inflammatory and Apoptotic Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shouqing Zhang,Jiajia Yu,Sule Bai,Shuhan Li,Quanyuan Qiu,Xiangshun Kong,Cen Xiang,Zhen Liu,Peng Yu,Yuou Teng

Diabetes, metabolic syndrome and obesity : targets 13:53-63 PubMed32021351

2020

The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Chunrong Zhao,Xiufeng Yin,Chunping Zhao

Blood 99:3256-62 PubMed11964291

2002

Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.

Applications

Unspecified application

Species

Unspecified reactive species

Maria J Polyak,Julie P Deans

View all publications

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Product promise

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Anti-EHHADH antibody

Immunoprecipitation - Anti-EHHADH antibody (AB123490)

Western blot - Anti-EHHADH antibody (AB123490)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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