Rabbit Polyclonal EHHADH antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EHHADH.
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- BioMed research international 2021:99170602021Increased EHHADH Expression Predicting Poor Survival of Osteosarcoma by Integrating Weighted Gene Coexpression Network Analysis and Experimental Validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuncheng Cui,Guoliang Yi,Jinxin Li,Yangtao Li,Dongyang QianPubMed 33997049
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EHHADH. The exact immunogen used to generate this antibody is proprietary information. Database link Q08426
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway (By similarity). Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (By similarity). Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed:15060085). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (PubMed:15060085).
Alternative names
ECHD, EHHADH, Peroxisomal bifunctional enzyme, PBE, PBFE, L-bifunctional protein, Multifunctional enzyme 1, LBP, MFE1
Recommended products
Rabbit Polyclonal EHHADH antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EHHADH.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EHHADH. The exact immunogen used to generate this antibody is proprietary information. Database link Q08426
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab136059 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EHHADH also known as enoyl-CoA hydratase/3-hydroxyacyl CoA dehydrogenase is a mitochondrial enzyme. Its molecular mass is approximately 82 kDa. EHHADH catalyzes two sequential reactions in the β-oxidation of fatty acids converting L-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. This enzyme expresses mainly in liver and kidney tissues playing a role in lipid metabolism.
- Biological function summary
EHHADH acts within the peroxisome a cellular organelle that facilitates lipid metabolism and detoxification processes. EHHADH is a part of the mitochondrial trifunctional protein complex. This complex helps in breaking down long-chain fatty acids highlighting EHHADH's role in energy production.
- Pathways
EHHADH engages in the fatty acid β-oxidation pathway and the peroxisomal fatty acid β-oxidation pathway. In these pathways EHHADH works alongside proteins like HADHA and HADHB both of which are part of the trifunctional protein complex. The collaboration between these proteins ensures efficient lipid degradation and energy release.
- Associated diseases and disorders
EHHADH links to disorders such as peroxisomal biogenesis disorders and Zellweger syndrome. Mutations affecting EHHADH or its associated proteins like VLCAD (very-long-chain acyl-CoA dehydrogenase) can disrupt normal mitochondrial functions. This disruption can lead to accumulation of unmetabolized fatty acids contributing to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-EHHADH antibody (ab136059)
All lanes: Western blot - Anti-EHHADH antibody (ab136059) at 1/500 dilution
All lanes: HepG2 cell lysate at 30 µg
Predicted band size: 79 kDa
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