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AB248843

Anti-EHM2 antibody [EP6191] - BSA and Azide free

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Rabbit Recombinant Monoclonal EHM2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

EHM2, LULU2, EPB41L4B, Band 4.1-like protein 4B, Erythrocyte membrane protein band 4.1-like 4B, FERM-containing protein CG1, Protein EHM2

1 Images
Western blot - Anti-EHM2 antibody [EP6191] - BSA and Azide free (AB248843)
  • WB

Unknown

Western blot - Anti-EHM2 antibody [EP6191] - BSA and Azide free (AB248843)

This data was developed using ab138487, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EHM2 antibody [EP6191] (<a href='/en-us/products/primary-antibodies/ehm2-antibody-ep6191-ab138487'>ab138487</a>) at 1/1000 dilution

Lane 1:

U87-MG cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP6191

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248843 is the carrier-free version of ab138487.

This antibody was developed as part of a collaboration with the lab of Andrei Ivanov at the University of Rochester.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHM2 also known as Emp24 domain-containing protein 2 is a mechanistic component of the vesicular trafficking process. Its molecular weight is approximately 27 kDa. This protein is mainly expressed in tissues such as the liver and heart. EHM2 plays a role in the transport of cargo molecules from the endoplasmic reticulum to the Golgi apparatus assisting in cellular organization and protein sorting.
Biological function summary

EHM2 functions as a part of the p24 protein complex which is essential for the vesicular transport system. This complex helps maintain the integrity and fidelity of transport vesicles. EHM2 interacts with the COPII and COPI coat protein complexes ensuring proper cargo selection and vesicle budding. It aids in maintaining cellular homeostasis through its contribution to efficient intracellular transport mechanisms.

Pathways

EHM2 participates in intracellular protein transport and secretion pathways. It plays a role in the ER-Golgi transport pathway and interacts with proteins like Sec24 and COPI integral components for vesicle formation and budding from the ER. These interactions facilitate the accurate transport of protein cargo and are necessary for maintaining protein distribution throughout the cell.

EHM2 shows connection to metabolic and cardiovascular diseases. Due to its expression in the liver and heart imbalances in EHM2 function may contribute to disorders such as fatty liver disease and cardiomyopathy. EHM2 interacts with proteins like apolipoproteins in these conditions potentially influencing lipid metabolism and cardiovascular health. Understanding EHM2's role could offer insights into therapeutic interventions for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Up-regulates the activity of the Rho guanine nucleotide exchange factor ARHGEF18 (By similarity). Involved in the regulation of the circumferential actomyosin belt in epithelial cells (PubMed : 22006950). Promotes cellular adhesion, migration and motility in vitro and may play a role in wound healing (PubMed : 23664528). May have a role in mediating cytoskeletal changes associated with steroid-induced cell differentiation (PubMed : 14521927).
See full target information EPB41L4B
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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