AB41969

Anti-EHMT1/GLP antibody [B0422]

Name: Anti-EHMT1/GLP antibody [B0422] (ab41969) | Abcam
Brand: Abcam Limited
SKU: AB41969
Price: 510 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(32 Publications)

Mouse Monoclonal EHMT1/GLP antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 32 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ehmt1 aa 100-250.

View Alternative Names

Euhmtase1, Glp, Kmt1d, Ehmt1, Histone-lysine N-methyltransferase EHMT1, Euchromatic histone-lysine N-methyltransferase 1, G9a-like protein 1, Lysine N-methyltransferase 1D, Eu-HMTase1, GLP, GLP1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-EHMT1/GLP antibody [B0422] (AB41969)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EHMT1/GLP antibody [B0422] (AB41969)

ab41969 at 5μg/ml staining GLP KO mouse ES cell (Negative).

This image was generated using the ascites version of the product.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EHMT1/GLP antibody [B0422] (AB41969)

ab41969 at 5 μg/ml staining WT mouse ES cell (Positive).

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B0422

Isotype

IgG2a

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Mouse Ehmt1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q5DW34

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 3^rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EHMT1 also known as GLP (G9a-like protein) is a histone methyltransferase involved in the methylation of histone H3 on lysine 9 a modification leading to transcriptional repression. The EHMT1 protein has a mass of approximately 150 kDa and is expressed in numerous tissues including the brain and heart. It functions as part of a larger complex and acts in the modification of chromatin structure and gene expression regulation.

Biological function summary

EHMT1/GLP plays a critical role in cellular processes such as development and differentiation. It forms a complex with G9a another histone methyltransferase to facilitate these functions. This complex cooperates in controlling gene silencing and the preservation of cellular identity. Such complex mechanisms lead to alterations in gene expression patterns that are essential for normal cellular operation.

Pathways

The methylation activity of EHMT1/GLP participates in the epigenetic regulation pathway and chromatin remodeling pathways. Within these pathways it interacts closely with proteins like HP1 which binds to methylated histones to promote gene silencing. The connections with pathways that influence gene expression affirm its role in maintaining cellular processes like proliferation and embryonic development.

Researchers connect EHMT1/GLP to Kleefstra syndrome—a condition characterized by intellectual disability and developmental delays. It is also associated with some cancer types due to its epigenetic regulation influences. In these contexts abnormal EHMT1/GLP activity often involving other proteins like G9a can contribute to the disruptions in gene regulation that lead to disease manifestations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins : mediates dimethylation of 'Lys-373' of p53/TP53. Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with probable chromatin reader Baz2b (PubMed : 32103178).

See full target information Ehmt1

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 43: PubMed37782747

2023

Writers and readers of H3K9me2 form distinct protein networks during the cell cycle that include candidates for H3K9 mimicry.

Applications

Unspecified application

Species

Unspecified reactive species

Gareth Pollin,Thiago M De Assuncao,Salomao Doria Jorge,Young-In Chi,M Cristine Charlesworth,Benjamin Madden,Juan Iovanna,Michael T Zimmermann,Raul Urrutia,Gwen Lomberk

Cancer research communications 3:1716-1730 PubMed37663929

2023

Demethylation of EHMT1/GLP Protein Reprograms Its Transcriptional Activity and Promotes Prostate Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Besschetnova,Wanting Han,Mingyu Liu,Yanfei Gao,Muqing Li,Zifeng Wang,Maryam Labaf,Susan Patalano,Kavita Venkataramani,Rachel E Muriph,Jill A Macoska,Kellee R Siegfried,Jason Evans,Steven P Balk,Shuai Gao,Dong Han,Changmeng Cai

Cancer research 83:1684-1698 PubMed36877164

2023

LSD1 Inhibition Disrupts Super-Enhancer-Driven Oncogenic Transcriptional Programs in Castration-Resistant Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Muqing Li,Mingyu Liu,Wanting Han,Zifeng Wang,Dong Han,Susan Patalano,Jill A Macoska,Steven P Balk,Housheng Hansen He,Eva Corey,Shuai Gao,Changmeng Cai

Epigenetics & chromatin 15:36 PubMed36411491

2022

De novo methylation of histone H3K23 by the methyltransferases EHMT1/GLP and EHMT2/G9a.

Applications

Unspecified application

Species

Unspecified reactive species

David A Vinson,Kimberly E Stephens,Robert N O'Meally,Shri Bhat,Blair C R Dancy,Robert N Cole,Srinivasan Yegnasubramanian,Sean D Taverna

International journal of biological sciences 18:4513-4531 PubMed35864958

2022

Maternal EHMT2 is essential for homologous chromosome segregation by regulating transcription in oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tie-Gang Meng,Wen-Long Lei,Xukun Lu,Xiao-Yu Liu,Xue-Shan Ma,Xiao-Qing Nie,Zheng-Hui Zhao,Qian-Nan Li,Lin Huang,Yi Hou,Ying-Chun Ouyang,Lei Li,Tie-Shan Tang,Heide Schatten,Wei Xie,Shao-Rong Gao,Xiang-Hong Ou,Zhen-Bo Wang,Qing-Yuan Sun

Frontiers in oncology 12:855167 PubMed35600406

2022

Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jyothishmathi Swaminathan,Shinji Maegawa,Shavali Shaik,Ajay Sharma,Javiera Bravo-Alegria,Lei Guo,Lin Xu,Arif Harmanci,Vidya Gopalakrishnan

Journal of immunology (Baltimore, Md. : 1950) 207:1437-1447 PubMed34400522

2021

Virus Infection Induces Keap1 Binding to Cytokine Genes, Which Recruits NF-κB p50 and G9a-GLP and Represses Cytokine Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Elizabeth Burns,Tom Klaus Kerppola

Nature communications 12:4359 PubMed34272378

2021

Complete loss of H3K9 methylation dissolves mouse heterochromatin organization.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Montavon,Nicholas Shukeir,Galina Erikson,Bettina Engist,Megumi Onishi-Seebacher,Devon Ryan,Yaarub Musa,Gerhard Mittler,Alexandra Graff Meyer,Christel Genoud,Thomas Jenuwein

Molecular cell 81:2944-2959.e10 PubMed34166609

2021

Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Lenka Skalska,Victoria Begley,Manuel Beltran,Saulius Lukauskas,Garima Khandelwal,Peter Faull,Amandeep Bhamra,Manuel Tavares,Rachel Wellman,Andrey Tvardovskiy,Benjamin M Foster,Igor Ruiz de Los Mozos,Javier Herrero,Silvia Surinova,Ambrosius P Snijders,Till Bartke,Richard G Jenner

Cell death & disease 12:409 PubMed33866326

2021

FGF23 ameliorates ischemia-reperfusion induced acute kidney injury via modulation of endothelial progenitor cells: targeting SDF-1/CXCR4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Huang-Ming Chang,Kang-Yung Peng,Chieh-Kai Chan,Chiao-Yin Sun,Ying-Ying Chen,Han-Mei Chang,Chun-Lin Huang,Pei-Chun Liu,Peng-Ying Chen,Kuo-Chuan Wang,Wei-Jie Wang,Chen-Chi Wu,Yu-Feng Lin,Tai-Shuan Lai,Tao-Min Huang,Guang-Huar Young,Shuei-Liong Lin,Marlies Ostermann,Tzong-Shinn Chu,Jeff S Chueh,Vin-Cent Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EHMT1/GLP antibody [B0422]

Immunocytochemistry/ Immunofluorescence - Anti-EHMT1/GLP antibody [B0422] (AB41969)

Immunocytochemistry/ Immunofluorescence - Anti-EHMT1/GLP antibody [B0422] (AB41969)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

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