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Rabbit Recombinant Monoclonal EHMT1/GLP antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.

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Images

Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (AB251187), expandable thumbnail
  • Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (AB251187), expandable thumbnail
  • Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (AB251187), expandable thumbnail
  • Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (AB251187), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (AB251187), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)IHC-PIP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Tested
Tested
Expected
Expected
Not recommended
Rat
Expected
Tested
Expected
Expected
Not recommended

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with probable chromatin reader BAZ2B (By similarity).

Additional Targets

EHMT2

Alternative names

EUHMTASE1, GLP, KIAA1876, KMT1D, EHMT1, Histone-lysine N-methyltransferase EHMT1, Euchromatic histone-lysine N-methyltransferase 1, G9a-like protein 1, Histone H3-K9 methyltransferase 5, Lysine N-methyltransferase 1D, Eu-HMTase1, GLP1, H3-K9-HMTase 5

Recommended products

Rabbit Recombinant Monoclonal EHMT1/GLP antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR18667
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251187 is the carrier-free version of Anti-EHMT2/G9A + EHMT1/GLP antibody [EPR18667] ab194299.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EHMT2 also known as G9a and EHMT1 referred to as GLP are lysine methyltransferases that add methyl groups to histone H3 on lysine 9 (H3K9). The functional proteins exist as dimers comprising a catalytic SET domain that mediates their enzymatic activity. EHMT2 has a mass of approximately 150 kDa while EHMT1 is about 143 kDa. These proteins are expressed in various tissues but show high expression levels in the brain liver and placenta. They play an essential role in chromatin modification which impacts gene expression.

Biological function summary

EHMT2 and EHMT1 form an important protein complex responsible for histone methylation. This complex regulates gene silencing through the tight modulation of chromatin structure affecting cellular processes such as differentiation and proliferation. EHMT2/G9a and EHMT1/GLP participate in establishing and maintaining transcriptional repression during development. The complex works in concert with other chromatin modifiers to control gene expression patterns contributing to the cellular and tissue-specific transcriptional programs.

Pathways

The functions of EHMT2 and EHMT1 integrate into the gene silencing pathways including the PRC2-mediated histone modification pathway. Within these pathways proteins like EZH2 a member of the Polycomb repressive complex 2 regulate histone methylation state further influencing gene expression patterns. EHMT2/G9a and EHMT1/GLP link signal transduction pathways to chromatin remodeling enabling cells to adapt their transcriptional activity according to external signals and developmental cues.

Associated diseases and disorders

Mutations or dysregulation of EHMT2 and EHMT1 have associations with neurological disorders like Kleefstra syndrome and certain cancers such as acute myeloid leukemia. The proteins' involvement in these disorders connects with other proteins like G9a and GLP through epigenetic silencing mechanisms emphasizing their influence on development and disease progression. Targeting EHMT2/G9a and EHMT1/GLP pathways can have potential therapeutic implications for treating these conditions highlighting the importance of understanding their functional roles in health and disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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