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AB187894

Anti-EI2BL/MRI1 antibody

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(2 Publications)

Rabbit Polyclonal EI2BL/MRI1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MRI1 aa 250 to C-terminus.

View Alternative Names

MRDI, UNQ6390/PRO21135, MRI1, Methylthioribose-1-phosphate isomerase, M1Pi, MTR-1-P isomerase, Mediator of RhoA-dependent invasion, S-methyl-5-thioribose-1-phosphate isomerase, Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EI2BL/MRI1 antibody (AB187894)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EI2BL/MRI1 antibody (AB187894)

Immunofluorescent analysis of Human cell line U-2 OS labeling EI2BL/MRI1 with ab187894 at 4μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-EI2BL/MRI1 antibody (AB187894)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EI2BL/MRI1 antibody (AB187894)

Immunofluorescent analysis of Human cell line U-2 OS labeling EI2BL/MRI1 with ab187894 at 4μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EI2BL/MRI1 antibody (AB187894)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EI2BL/MRI1 antibody (AB187894)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human thyroid gland tssue labeling EI2BL/MRI1 with ab187894 at 1/1000 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MRI1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BV20

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EI2BL/MRI1 also known as TRMT11 serves as a methyltransferase enzyme with a molecular mass of approximately 68 kDa. It primarily functions in the methylation of guanosine at the N2 position within tRNA molecules. Expression of this protein occurs mainly in the cytoplasm but it can also be found in the nucleus of various cellular types including human tissue.
Biological function summary

EI2BL/MRI1 plays a role in the biosynthesis and modification of tRNA which is essential for accurate protein synthesis. It is part of a complex that functions with methyltransferases to ensure proper methylation of RNA ultimately supporting translation fidelity. This activity helps maintain the stability and integrity of tRNA molecules necessary for protein production.

Pathways

EI2BL/MRI1 actively participates in the RNA modification and processing pathways specifically in tRNA methylation. It interrelates with other proteins such as tRNase Z and METTL1 that are also involved in RNA processing. These interactions are critical for maintaining the proper function of the translation machinery by ensuring properly modified tRNA molecules are available.

Disruptions in EI2BL/MRI1 have implications in disorders related to protein synthesis malfunction. For example aberrant tRNA methylation linked to this protein has associations with certain types of cancer where transcriptional and translational regulation are compromised. Moreover its interaction with METTL1 can influence oncogenic processes suggesting EI2BL/MRI1 as a potential target for therapeutic intervention in cancer treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P). Independently from catalytic activity, promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation and stress fiber turnover.
See full target information MRI1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical pathology 73:139-146 PubMed31586937

2019

Overexpression of EI2BL promoted human non-small cell lung cancer progression by inducing cell EMT phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Ran Li,Bai-Quan Qiu,Jian Gao,Chun Jin,Jia-Hao Jiang,Jian-Yong Ding

Genes & development 33:1381-1396 PubMed31488579

2019

, the nuclear exosome targeting component, is mutated in familial pulmonary fibrosis and is required for telomerase RNA maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Dustin L Gable,Valeriya Gaysinskaya,Christine C Atik,C Conover Talbot,Byunghak Kang,Susan E Stanley,Elizabeth W Pugh,Nuria Amat-Codina,Kara M Schenk,Murat O Arcasoy,Cory Brayton,Liliana Florea,Mary Armanios
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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