AB78323

Anti-EID1 antibody [26]

Name: Anti-EID1 antibody [26] (ab78323) | Abcam
Brand: Abcam Limited
SKU: AB78323
Price: 585 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal EID1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EID1 aa 150 to C-terminus.

View Alternative Names

C15orf3, CRI1, RBP21, PNAS-22, PTD014, EID1, EP300-interacting inhibitor of differentiation 1, 21 kDa pRb-associated protein, CREBBP/EP300 inhibitory protein 1, E1A-like inhibitor of differentiation 1, EID-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-EID1 antibody [26] (AB78323)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EID1 antibody [26] (AB78323)

ab78323 staining EID1 in HeLA cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with Triton X-100 0.25% in PBS and blocked with 1.5% BSA for 30 minutes. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) overnight at 4°C. An Alexa Fluor^®488-conjugated Goat anti-mouse IgG polyclonal (1/1000 in PBS + 1% BSA) was used as the secondary antibody incubated for 1 hour. DAPI was used to stain the nuclear DNA.

Unknown

Western blot - Anti-EID1 antibody [26] (AB78323)

All lanes:

Western blot - Anti-EID1 antibody [26] (ab78323) at 1 µg/mL

Lane 1:

extracts from MCF7 cells transfected with control vector pCMV1

Lane 2:

extracts from MCF7 cells transfected with the EID1 expression vector pcDNA3/EID1

Secondary

All lanes:

HRP-conjugated mouse IgG

Predicted band size: 21 kDa

Observed band size: 21 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human EID1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6B2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

ab78323 was purified by propriety chromatography under mild conditions as IgG fraction from serum free growth medium of mouse hybridoma clone and sterilized by filtration.

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EID1 also known as EP300 interacting inhibitor of differentiation 1 is a nuclear protein involved in transcriptional regulation. It has a molecular mass of approximately 22 kDa. EID1 primarily expresses in various tissues and acts as a co-regulator by interacting with transcription factors and modifying the transcription process. Its ability to bind with different cofactors allows it to play a role in multiple cellular functions.

Biological function summary

EID1 modulates transcriptional repression and differentiation processes. It participates in complexes with other proteins such as histone deacetylases and methyltransferases to alter chromatin structure and gene expression. EID1 can inhibit the activity of certain transcription factors influencing cell cycle progression and developmental pathways. In these roles EID1 exerts a substantial impact on cellular differentiation and proliferation.

Pathways

EID1 significantly impacts pathways like retinoblastoma (RB) and Wnt signaling. In the RB pathway EID1 modulates cell cycle control by interacting with proteins like p300/CBP. EID1's role in the Wnt signaling pathway involves influencing β-catenin activity which impacts cell fate determination and stem cell renewal. These interactions highlight EID1's role in signaling cascades that regulate proliferation and differentiation.

EID1 associates with cancer and neurodegenerative diseases. In cancer EID1 can alter tumor suppressor pathways by interacting with proteins such as p300 potentially affecting the tumor's growth and progression. In neurodegenerative diseases its role in gene expression modulation may result in atypical cellular functions that contribute to disease development. EID1's interactions with various co-regulators make it a target for research in diseased states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.

See full target information EID1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 21:219 PubMed37612584

2023

RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Hyun Jo,Jong-Uk Park,Yeong-Mu Kim,Seon-Mi Ok,Dong-Kyu Kim,Dong-Hyun Jung,Hye-Ji Kim,Hyun-A Seong,Hyo Je Cho,Jihoon Nah,Sangjune Kim,Haiqing Fu,Christophe E Redon,Mirit I Aladjem,Sang-Min Jang

Journal of cellular physiology 235:6725-6735 PubMed32056205

2020

EID1 suppresses lipid accumulation by inhibiting the expression of GPDH in 3T3-L1 preadipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tomohiko Sato,Diana Vargas,Kakushin Miyazaki,Kaoru Uchida,Winda Ariyani,Mitsue Miyazaki,Junichi Okada,Fernando Lizcano,Noriyuki Koibuchi,Noriaki Shimokawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EID1 antibody [26]

Immunocytochemistry/ Immunofluorescence - Anti-EID1 antibody [26] (AB78323)

Western blot - Anti-EID1 antibody [26] (AB78323)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise