Rabbit Recombinant Monoclonal eIF-6 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (AB248473), expandable thumbnail](https://content.abcam.com/products/images/eif-6-antibody-epr6513-bsa-and-azide-free-ab248473--western-blot-img142101.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (AB248473)")
![OI-RD Scanning - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (AB248473), expandable thumbnail](https://content.abcam.com/products/images/eif-6-antibody-epr6513-bsa-and-azide-free-ab248473--oi-rd-scanning-img157576.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (AB248473)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm (PubMed:10085284, PubMed:14654845, PubMed:21536732, PubMed:32669547). Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity (PubMed:10085284, PubMed:14654845, PubMed:21536732). In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (By similarity). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:17507929). Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (By similarity).
Alternative names
EIF3A, ITGB4BP, OK/SW-cl.27, EIF6, Eukaryotic translation initiation factor 6, eIF-6, B(2)GCN homolog, B4 integrin interactor, CAB, p27(BBP)
Recommended products
Rabbit Recombinant Monoclonal eIF-6 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6513
- Purification technique
- Affinity purification
- Dissociation constant
- 5.7 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248473 is the carrier-free version of Anti-eIF-6 antibody [EPR6513] ab133321.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF-6 also known as eukaryotic translation initiation factor 6 is a protein involved in the control of ribosomal function. The protein has a molecular mass of approximately 25 kDa and is expressed in the cytoplasm of eukaryotic cells. It acts by binding to the 60S ribosomal subunit and preventing premature association with the 40S subunit. This regulation ensures the proper assembly and function of the 80S ribosome essential for protein synthesis.
- Biological function summary
Eukaryotic translation initiation factor 6 plays a significant role in ribosome biogenesis and translation initiation. It functions as a part of the ribosomal precursor complex facilitating the formation of functional 60S ribosomal units. Through its action eIF-6 modulates cellular protein synthesis rates impacting cell growth and proliferation. Additionally eIF-6 influences cellular responses to nutrient availability stressing its role in metabolic regulation.
- Pathways
Eukaryotic translation initiation factor 6 is integral to the mTOR signaling pathway and ribosome biogenesis pathway. The mTOR pathway involves proteins like mTOR itself and S6 kinase which connect to eIF-6-mediated regulation of protein synthesis. eIF-6 maintains the balance between ribosome production and protein translation rates coordinating cellular growth with environmental cues.
- Associated diseases and disorders
Aberrations in eukaryotic translation initiation factor 6 levels link to cancer and metabolic diseases. Overexpression of eIF-6 can lead to excessive protein synthesis contributing to oncogenic processes by promoting unchecked cell proliferation. Additionally the dysregulation of eIF-6 can affect the mTOR pathway contributing to metabolic dysfunction. Connections between eIF-6 and other proteins like cyclin D1 highlight its impact on tumorigenesis and cell cycle control.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473), expandable thumbnail](https://content.abcam.com/products/images/eif-6-antibody-epr6513-bsa-and-azide-free-ab248473--western-blot-img142101.jpg "Western blot - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)")
Western blot - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
This data was developed using Anti-eIF-6 antibody [EPR6513] ab133321, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473) at 1/10000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDa
![OI-RD Scanning - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473), expandable thumbnail](https://content.abcam.com/products/images/eif-6-antibody-epr6513-bsa-and-azide-free-ab248473--oi-rd-scanning-img157576.jpg "OI-RD Scanning - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)")
OI-RD Scanning - Anti-eIF-6 antibody [EPR6513] - BSA and Azide free (ab248473)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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