Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling EIF1AX with ab177939 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

This image was generated using the unpurified version of the product.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrium cancer tissue sections labeling eIF1A with Purified ab177939 at 1 : 350 dilution (1.61 μg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling eIF1A with Purified ab177939 at 1/60 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)

Immunofluorescent analysis of HeLa cells labeling EIF1AX with ab177939 at 1/50 dilution (red). DAPI nuclear staining (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

This image was generated using the unpurified version of the product.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EIF1AX with ab177939 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

This image was generated using the unpurified version of the product.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)

Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling eIF1A with Purified ab177939 at 1 : 100 dilution (5.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

• IP

Supplier Data

Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)

Western blot analysis on immunoprecipitation pellet from A375 cell lysate immunoprecipitated using ab177939 at 1/10 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

This image was generated using the unpurified version of the product.

All lanes:

Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] (<a href='/en-us/products/primary-antibodies/eif1a-antibody-epr12466b-ab177939'>ab177939</a>)

Predicted band size: 16 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)

ab177939 (purified) at 1 : 30 dilution (2μg) immunoprecipitating eIF1A in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab177939 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab177939 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

All lanes:

Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] (<a href='/en-us/products/primary-antibodies/eif1a-antibody-epr12466b-ab177939'>ab177939</a>)

Predicted band size: 16 kDa

false