Rabbit Recombinant Monoclonal eIF1A antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunoprecipitation-img9153.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85387.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)")
![Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunocytochemistry-immunofluorescence-img97303.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)")
![Flow Cytometry (Intracellular) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--flow-cytometry-intracellular-img32922.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109876.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (AB243919)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
| Mouse | Predicted | Predicted | Expected | Predicted | Predicted |
| Rat | Predicted | Predicted | Expected | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Species Rat | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Species Human | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon (PubMed:9732867). This protein enhances formation of the cap-proximal complex (PubMed:9732867). Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes (PubMed:9732867). After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex (PubMed:35732735). Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B (PubMed:35732735). Its globular part is located in the A site of the 40S ribosomal subunit (PubMed:35732735). Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC (PubMed:24319994). In contrast to yeast orthologs, does not bind EIF1 (PubMed:24319994).
Alternative names
EIF1A, EIF4C, EIF1AX, eIF-1A X isoform, eIF1A X isoform, Eukaryotic translation initiation factor 4C, eIF-4C
Recommended products
Rabbit Recombinant Monoclonal eIF1A antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12466(B)
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab243919 is the carrier-free version of Anti-eIF1A antibody [EPR12466(B)] ab177939.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF1A also known as Eukaryotic Initiation Factor 1A plays an important role in the process of initiating translation. It has a mass of approximately 17.5 kDa. This factor is expressed in various tissues playing a role in forming the pre-initiation complex ensuring mRNA and ribosomal alignment. eIF1A's structure and binding capabilities make it an essential component for the fidelity and efficiency of translation initiation.
- Biological function summary
EIF1A functions as part of the eukaryotic translation initiation complex. It stabilizes the binding of the small ribosomal subunit to mRNA. eIF1A works closely with other initiation factors including eIF1 eIF3 and eIF5 to facilitate the proper scanning of mRNA for the correct start codon. This precise role in translation initiation serves as a regulatory point for protein synthesis in cells.
- Pathways
EIF1A engages in the mRNA translation pathway particularly in the initiation phase. It is critical in ensuring the mRNA translation accuracy by preventing premature joining of the large ribosomal subunit. eIF1A associates with proteins like eIF3 and eIF2 during the initial stages of translation. This involvement ensures cellular homeostasis and efficient cellular response to various stimuli.
- Associated diseases and disorders
EIF1A may influence cancer development and progression. Alterations in its expression levels or mutations can disrupt normal translation processes potentially leading to oncogenesis. Research indicates a connection between eIF1A and tumor suppressor proteins highlighting its impact on cellular proliferation. Additionally eIF1A may relate to neurodegenerative disorders by affecting proteins such as eIF2 impacting neuronal translation control and protein synthesis regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunoprecipitation-img9153.jpg "Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
Anti-eIF1A antibody [EPR12466(B)] ab177939 (purified) at 1:30 dilution (2μg) immunoprecipitating eIF1A in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): Anti-eIF1A antibody [EPR12466(B)] ab177939 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-eIF1A antibody [EPR12466(B)] ab177939 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF1A antibody [EPR12466(B)] ab177939)
All lanes: Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] (Anti-eIF1A antibody [EPR12466(B)] ab177939)
Predicted band size: 16 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85387.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrium cancer tissue sections labeling eIF1A with Purified Anti-eIF1A antibody [EPR12466(B)] ab177939 at 1:350 dilution (1.61 μg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF1A antibody [EPR12466(B)] ab177939)
![Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunocytochemistry-immunofluorescence-img97303.jpg "Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling eIF1A with Purified Anti-eIF1A antibody [EPR12466(B)] ab177939 at 1:100 dilution (5.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF1A antibody [EPR12466(B)] ab177939)
![Flow Cytometry (Intracellular) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--flow-cytometry-intracellular-img32922.jpg "Flow Cytometry (Intracellular) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
Flow Cytometry (Intracellular) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling eIF1A with Purified Anti-eIF1A antibody [EPR12466(B)] ab177939 at 1/60 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF1A antibody [EPR12466(B)] ab177939)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109876.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling EIF1AX with Anti-eIF1A antibody [EPR12466(B)] ab177939 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF1A antibody [EPR12466(B)] ab177939)
This image was generated using the unpurified version of the product.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunoprecipitation-img70356.jpg "Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
Western blot analysis on immunoprecipitation pellet from A375 cell lysate immunoprecipitated using Anti-eIF1A antibody [EPR12466(B)] ab177939 at 1/10 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF1A antibody [EPR12466(B)] ab177939)
This image was generated using the unpurified version of the product.
All lanes: Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] (Anti-eIF1A antibody [EPR12466(B)] ab177939)
Predicted band size: 16 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111695.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EIF1AX with Anti-eIF1A antibody [EPR12466(B)] ab177939 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF1A antibody [EPR12466(B)] ab177939)
This image was generated using the unpurified version of the product.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunocytochemistry-immunofluorescence-img133146.jpg "Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
Immunofluorescent analysis of HeLa cells labeling EIF1AX with Anti-eIF1A antibody [EPR12466(B)] ab177939 at 1/50 dilution (red). DAPI nuclear staining (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF1A antibody [EPR12466(B)] ab177939)
This image was generated using the unpurified version of the product.
![Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunoprecipitation-img70356.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111695.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
![Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919), expandable thumbnail](https://content.abcam.com/products/images/eif1a-antibody-epr12466b-bsa-and-azide-free-ab243919--immunocytochemistry-immunofluorescence-img133146.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com