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AB172623

Anti-eIF1A antibody [EPR12467]

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(4 Publications)

Rabbit Recombinant Monoclonal eIF1A antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

EIF1A, EIF4C, EIF1AX, eIF-1A X isoform, eIF1A X isoform, Eukaryotic translation initiation factor 4C, eIF-4C

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (AB172623)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (AB172623)

Immunofluorescent analysis of A375 cells labeling eIF1A with ab172623 at 1/50 dilution (green) and counterstained with DAPI (blue)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (AB172623)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (AB172623)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling eIF1A with ab172623 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (AB172623)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (AB172623)

Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue labeling eIF1A with ab172623 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (AB172623)
  • IP

Supplier Data

Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (AB172623)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling eIF1A with ab172923 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)

Predicted band size: 16 kDa

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Western blot - Anti-eIF1A antibody [EPR12467] (AB172623)
  • WB

Supplier Data

Western blot - Anti-eIF1A antibody [EPR12467] (AB172623)

All lanes:

Western blot - Anti-eIF1A antibody [EPR12467] (ab172623) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

A375 cell lysate at 10 µg

Predicted band size: 16 kDa

false

Western blot - Anti-eIF1A antibody [EPR12467] (AB172623)
  • WB

CiteAb

Western blot - Anti-eIF1A antibody [EPR12467] (AB172623)

eIF1A western blot using anti-eIF1A antibody [EPR12467] ab172623. Publication image and figure legend from del Puerto-Nevado, L., Santiago-Hernandez, A., et al., 2019, Mol Oncol, PubMed 31199051.

ab172623 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab172623 please see the product overview.

Western blot analysis of MRPL18 (A), TIMM8B (B), and EIF1A (C) from in vitro experiments using a normal colon mucosa epithelial cell line exposed to high (HG) or NG condition. Relative densitometric quantification and representative immunoblots are shown. CO : (osmotic) control. Error bars indicate SEM. Mann–Whitney statistical U-test used with three biologically independent replicates included (*P-value < 0.05).

false

  • Carrier free

    Anti-eIF1A antibody [EPR12467] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12467

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF1A also known as Eukaryotic Initiation Factor 1A plays an important role in the process of initiating translation. It has a mass of approximately 17.5 kDa. This factor is expressed in various tissues playing a role in forming the pre-initiation complex ensuring mRNA and ribosomal alignment. eIF1A's structure and binding capabilities make it an essential component for the fidelity and efficiency of translation initiation.
Biological function summary

EIF1A functions as part of the eukaryotic translation initiation complex. It stabilizes the binding of the small ribosomal subunit to mRNA. eIF1A works closely with other initiation factors including eIF1 eIF3 and eIF5 to facilitate the proper scanning of mRNA for the correct start codon. This precise role in translation initiation serves as a regulatory point for protein synthesis in cells.

Pathways

EIF1A engages in the mRNA translation pathway particularly in the initiation phase. It is critical in ensuring the mRNA translation accuracy by preventing premature joining of the large ribosomal subunit. eIF1A associates with proteins like eIF3 and eIF2 during the initial stages of translation. This involvement ensures cellular homeostasis and efficient cellular response to various stimuli.

EIF1A may influence cancer development and progression. Alterations in its expression levels or mutations can disrupt normal translation processes potentially leading to oncogenesis. Research indicates a connection between eIF1A and tumor suppressor proteins highlighting its impact on cellular proliferation. Additionally eIF1A may relate to neurodegenerative disorders by affecting proteins such as eIF2 impacting neuronal translation control and protein synthesis regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon (PubMed : 9732867). This protein enhances formation of the cap-proximal complex (PubMed : 9732867). Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes (PubMed : 9732867). After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex (PubMed : 35732735). Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B (PubMed : 35732735). Its globular part is located in the A site of the 40S ribosomal subunit (PubMed : 35732735). Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC (PubMed : 24319994). In contrast to yeast orthologs, does not bind EIF1 (PubMed : 24319994).
See full target information EIF1AX
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1520070 PubMed40018039

2025

Integrative analysis of m7G methylation-associated genes prognostic signature with immunotherapy and identification of LARP1 as a key oncogene in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xu,Zihao You,Zhongping Zhu,Min Liu,Zheng Zhang,Panpan Xu,Juanjuan Dong,Yuting Huang,Chao Wang,Haotian Qin

Molecular oncology 13:1887-1897 PubMed31199051

2019

Diabetes-mediated promotion of colon mucosa carcinogenesis is associated with mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Del Puerto-Nevado,Aranzazu Santiago-Hernandez,Sonia Solanes-Casado,Nieves Gonzalez,Marta Ricote,Marta Corton,Isabel Prieto,Sebastian Mas,Ana Belen Sanz,Oscar Aguilera,Carmen Gomez-Guerrero,Carmen Ayuso,Alberto Ortiz,Federico Rojo,Jesus Egido,Jesus Garcia-Foncillas,Pablo Minguez,Gloria Alvarez-Llamas

Frontiers in microbiology 9:207 PubMed29487587

2018

The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Esther González-Almela,Hugh Williams,Miguel A Sanz,Luis Carrasco

Nature communications 6:7194 PubMed26018492

2015

eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference.

Applications

WB, IP

Species

Unspecified reactive species, Unspecified reactive species

Tingfang Yi,Haribabu Arthanari,Barak Akabayov,Huaidong Song,Evangelos Papadopoulos,Hank H Qi,Mark Jedrychowski,Thomas Güttler,Cuicui Guo,Rafael E Luna,Steven P Gygi,Stephen A Huang,Gerhard Wagner
View all publications
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Product promise

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