Rabbit Polyclonal EIF1AY antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14602
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Alternatively Tris-EDTA buffer (pH8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits (By similarity).
Alternative names
eIF-1A Y isoform, Eukaryotic translation initiation factor 4C, eIF-4C, EIF1AY
Recommended products
Rabbit Polyclonal EIF1AY antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus.
Alternative names
eIF-1A Y isoform, Eukaryotic translation initiation factor 4C, eIF-4C, EIF1AY
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14602
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
EIF1AY also known as eukaryotic translation initiation factor 1A Y-linked is a protein that plays a critical role in the initiation of protein synthesis. It has a molecular mass of about 17 kDa and is expressed specifically on the Y chromosome making it uniquely present in males. EIF1AY is part of the translation initiation complex where it facilitates the recruitment and correct positioning of the ribosome on mRNA a step essential for the formation of the pre-initiation complex in protein synthesis.
- Biological function summary
EIF1AY contributes to cellular translation processes by forming a complex with other translation initiation factors. This complex stabilizes the mRNA binding and enables the scanning process for the start codon which is an important step in initiating translation. Through these interactions EIF1AY ensures the fidelity and efficiency of protein synthesis which is fundamental for cell growth and proliferation.
- Pathways
EIF1AY is involved in the mTOR signaling pathway which regulates growth and metabolism in response to environmental cues. This pathway involves other important translation initiation factors including eIF4E and eIF4G which work together to control protein synthesis in response to nutrients and growth factors. EIF1AY also plays a role in the integrated stress response pathway which adjusts protein synthesis rates during cellular stress conditions by modulating eukaryotic initiation factors.
- Associated diseases and disorders
Disruptions in EIF1AY function are linked to certain male-specific conditions given its Y-linked expression. This includes infertility issues attributable to defects in spermatogenesis where EIF1AY’s role in protein synthesis is critical. EIF1AY may also relate to disorders involving aberrant protein synthesis in connection with proteins like eIF2 which regulates the initiation phase under stress conditions. Understanding these connections can provide insights into targeted treatments for affected conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-EIF1AY antibody (ab155546)
15% SDS PAGE
All lanes: Western blot - Anti-EIF1AY antibody (ab155546) at 1/500 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF1AY antibody (ab155546)
Immunohistochemical analysis of paraffin-embedded Human Lung AdCA, labeling EIF1AY using ab155546 at 1/500 dilution.
Western blot - Anti-EIF1AY antibody (ab155546)
15 % SDS-PAGE
All lanes: Western blot - Anti-EIF1AY antibody (ab155546) at 1/1000 dilution
All lanes: PC-12 whole cell lysate at 30 µg
Predicted band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-EIF1AY antibody (ab155546)
Immunofluorescent analysis of methanol fixed A431 cells, labeling EIF1AY with ab155546 at 1/500 dilution (green).
A431 cells were fixed in ice-cold Methanol for 5 min.
Right panel shows Hoechst 33343 staining (blue).
Downloads
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