JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155546

Anti-EIF1AY antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal EIF1AY antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus.

View Alternative Names

eIF-1A Y isoform, eIF1A Y isoform, Eukaryotic translation initiation factor 4C, eIF-4C, EIF1AY

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-EIF1AY antibody (AB155546)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EIF1AY antibody (AB155546)

Immunofluorescent analysis of methanol fixed A431 cells, labeling EIF1AY with ab155546 at 1/500 dilution (green). A431 cells were fixed in ice-cold Methanol for 5 min. Right panel shows Hoechst 33343 staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF1AY antibody (AB155546)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF1AY antibody (AB155546)

Immunohistochemical analysis of paraffin-embedded Human Lung AdCA, labeling EIF1AY using ab155546 at 1/500 dilution.

Western blot - Anti-EIF1AY antibody (AB155546)
  • WB

Unknown

Western blot - Anti-EIF1AY antibody (AB155546)

15% SDS PAGE

All lanes:

Western blot - Anti-EIF1AY antibody (ab155546) at 1/500 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 16 kDa

false

Western blot - Anti-EIF1AY antibody (AB155546)
  • WB

Unknown

Western blot - Anti-EIF1AY antibody (AB155546)

15 % SDS-PAGE

All lanes:

Western blot - Anti-EIF1AY antibody (ab155546) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 30 µg

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O14602

style
left-column

Complementary Products

Primary Antibodies

AB101518

Anti-ATPAF1 antibody

Western blot - Anti-ATPAF1 antibody (AB101518)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB31217

Anti-eIF4A1 antibody

Western blot - Anti-eIF4A1 antibody (AB31217)

  • 5
  • 6
Primary Antibodies

AB169528

Anti-eIF2A antibody [EPR11042]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody [EPR11042] (AB169528)

  • 5
  • 5
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Alternatively Tris-EDTA buffer (pH8.0) may be used.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF1AY also known as eukaryotic translation initiation factor 1A Y-linked is a protein that plays a critical role in the initiation of protein synthesis. It has a molecular mass of about 17 kDa and is expressed specifically on the Y chromosome making it uniquely present in males. EIF1AY is part of the translation initiation complex where it facilitates the recruitment and correct positioning of the ribosome on mRNA a step essential for the formation of the pre-initiation complex in protein synthesis.
Biological function summary

EIF1AY contributes to cellular translation processes by forming a complex with other translation initiation factors. This complex stabilizes the mRNA binding and enables the scanning process for the start codon which is an important step in initiating translation. Through these interactions EIF1AY ensures the fidelity and efficiency of protein synthesis which is fundamental for cell growth and proliferation.

Pathways

EIF1AY is involved in the mTOR signaling pathway which regulates growth and metabolism in response to environmental cues. This pathway involves other important translation initiation factors including eIF4E and eIF4G which work together to control protein synthesis in response to nutrients and growth factors. EIF1AY also plays a role in the integrated stress response pathway which adjusts protein synthesis rates during cellular stress conditions by modulating eukaryotic initiation factors.

Disruptions in EIF1AY function are linked to certain male-specific conditions given its Y-linked expression. This includes infertility issues attributable to defects in spermatogenesis where EIF1AY’s role in protein synthesis is critical. EIF1AY may also relate to disorders involving aberrant protein synthesis in connection with proteins like eIF2 which regulates the initiation phase under stress conditions. Understanding these connections can provide insights into targeted treatments for affected conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B. Its globular part is located in the A site of the 40S ribosomal subunit. Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC. In contrast to yeast orthologs, does not bind EIF1.
See full target information EIF1AY
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of clinical oncology 15:755-764 PubMed38946832

2024

Tankyrase 2 promotes lung cancer cell malignancy.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Yong-Jun Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com