Rabbit Polyclonal EIF1AY antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14602
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Alternatively Tris-EDTA buffer (pH8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B. Its globular part is located in the A site of the 40S ribosomal subunit. Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC. In contrast to yeast orthologs, does not bind EIF1.
Alternative names
eIF-1A Y isoform, eIF1A Y isoform, Eukaryotic translation initiation factor 4C, eIF-4C, EIF1AY
Recommended products
Rabbit Polyclonal EIF1AY antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EIF1AY aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O14602
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF1AY also known as eukaryotic translation initiation factor 1A Y-linked is a protein that plays a critical role in the initiation of protein synthesis. It has a molecular mass of about 17 kDa and is expressed specifically on the Y chromosome making it uniquely present in males. EIF1AY is part of the translation initiation complex where it facilitates the recruitment and correct positioning of the ribosome on mRNA a step essential for the formation of the pre-initiation complex in protein synthesis.
- Biological function summary
EIF1AY contributes to cellular translation processes by forming a complex with other translation initiation factors. This complex stabilizes the mRNA binding and enables the scanning process for the start codon which is an important step in initiating translation. Through these interactions EIF1AY ensures the fidelity and efficiency of protein synthesis which is fundamental for cell growth and proliferation.
- Pathways
EIF1AY is involved in the mTOR signaling pathway which regulates growth and metabolism in response to environmental cues. This pathway involves other important translation initiation factors including eIF4E and eIF4G which work together to control protein synthesis in response to nutrients and growth factors. EIF1AY also plays a role in the integrated stress response pathway which adjusts protein synthesis rates during cellular stress conditions by modulating eukaryotic initiation factors.
- Associated diseases and disorders
Disruptions in EIF1AY function are linked to certain male-specific conditions given its Y-linked expression. This includes infertility issues attributable to defects in spermatogenesis where EIF1AY’s role in protein synthesis is critical. EIF1AY may also relate to disorders involving aberrant protein synthesis in connection with proteins like eIF2 which regulates the initiation phase under stress conditions. Understanding these connections can provide insights into targeted treatments for affected conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-EIF1AY antibody (ab155546)
15% SDS PAGE
All lanes: Western blot - Anti-EIF1AY antibody (ab155546) at 1/500 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF1AY antibody (ab155546)
Immunohistochemical analysis of paraffin-embedded Human Lung AdCA, labeling EIF1AY using ab155546 at 1/500 dilution.
Western blot - Anti-EIF1AY antibody (ab155546)
15 % SDS-PAGE
All lanes: Western blot - Anti-EIF1AY antibody (ab155546) at 1/1000 dilution
All lanes: PC-12 whole cell lysate at 30 µg
Predicted band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-EIF1AY antibody (ab155546)
Immunofluorescent analysis of methanol fixed A431 cells, labeling EIF1AY with ab155546 at 1/500 dilution (green).
A431 cells were fixed in ice-cold Methanol for 5 min.
Right panel shows Hoechst 33343 staining (blue).
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