AB181467

Anti-eIF2A antibody [3A7B11]

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(3 Publications)

Mouse Monoclonal EIF2A antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat, African green monkey samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF2A aa 400 to C-terminus.

View Alternative Names

CDA02, MSTP004, MSTP089, EIF2A, Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A

7 Images

Flow Cytometry - Anti-eIF2A antibody [3A7B11] (AB181467)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-eIF2A antibody [3A7B11] (AB181467)

Flow cytometric analysis of HepG2 cells labeling eIF2A with ab181467 at 1/200 dilution (green) compared to a negative control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (AB181467)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (AB181467)

Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (AB181467)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.

Lab

Western blot - Anti-eIF2A antibody [3A7B11] (AB181467)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : eIF2A knockout HAP1 cell lysate (20 μg)
Lane 3 : Ramos cell lysate (20 μg)
Lane 4 : MOLT4 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab181467 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab181467 was shown to recognize eIF2A when eIF2A knockout samples were used, along with additional cross-reactive bands. Wild-type and eIF2A knockout samples were subjected to SDS-PAGE. ab181467 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)

Predicted band size: 65 kDa

false

Supplier Data

Western blot - Anti-eIF2A antibody [3A7B11] (AB181467)

All lanes:

Western blot - Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

Lane 1:

MCF7 cell lysate

Lane 2:

PC-12 cell lysate

Lane 3:

HepG2 cell lysate

Lane 4:

HeLa cell lysate

Lane 5:

COS-7 cell lysate

Lane 6:

K562 cell lysate

Lane 7:

Jurkat cell lysate

Lane 8:

A431 cell lysate

Lane 9:

NIH3T3 cell lysate

Predicted band size: 65 kDa

false

Supplier Data

Western blot - Anti-eIF2A antibody [3A7B11] (AB181467)

Expected MWt is 40.3 kDa.

All lanes:

Western blot - Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

All lanes:

Recombinant Human eIF2A (aa 448-576) protein

Predicted band size: 65 kDa

false

Supplier Data

Western blot - Anti-eIF2A antibody [3A7B11] (AB181467)

All lanes:

Western blot - Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

Lane 1:

Non-transfected HEK293 cell lysate

Lane 2:

eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 65 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A7B11

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human, African green monkey

Applications

IHC-P, Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human EIF2A aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BY44

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eIF2A also referred to as eukaryotic translation initiation factor 2A is a protein involved in the initiation of mRNA translation. The eIF2A protein is distinct from eIF2α despite the similarity in their names. Its molecular weight is approximately 65 kDa. Expression of eIF2A occurs mainly in the cytoplasm of cells where it participates in the protein synthesis process. eIF2A functions as a mediator that targets the initiator methionylated-tRNA to the small ribosomal subunit in a manner independent from eIF2 complex.

Biological function summary

EIF2A impacts the translation mechanism by facilitating the binding of Met-tRNAi to the ribosome under particular conditions especially during stress responses where canonical eIF2 might be inhibited. It operates independently from the larger eIF2 complex which also includes eIF2β and eIF2γ subunits. This independent activity allows eIF2A to serve as a backup mechanism for translation initiation when the normal pathway is impaired.

Pathways

Translation regulation involves interaction with cellular stress pathways and the integrated stress response (ISR). eIF2A operates within pathways that respond to physiological stresses such as nutrient deprivation and oxidative stress which trigger alterations in protein synthesis rates. eIF2A is related to the eIF2 kinase which phosphorylates the α subunit of eIF2 leading to a general reduction in translation initiation under stress allowing selective translation of stress-response proteins.

Irregularities in eIF2A function link to neurodegenerative diseases such as Alzheimer's disease and metabolic disorders like diabetes. These conditions often involve disruptions in the stress response pathways where eIF2A participates. The protein participates with others like PERK a kinase that phosphorylates eIF2α under endoplasmic reticulum stress contributing to disease-related changes in protein synthesis. By modulating translation under stress eIF2A becomes significant in these pathological contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.

See full target information EIF2A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:13555 PubMed35941362

2022

ID1 marks the tumorigenesis of pancreatic ductal adenocarcinoma in mouse and human.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanxin Tang,Sheng Zhang,Jiazi Li,Chunli Wu,Qing Fan

International journal of cancer 149:657-674 PubMed33837564

2021

FOXD1 promotes dedifferentiation and targeted therapy resistance in melanoma by regulating the expression of connective tissue growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Sun,Daniel Novak,Laura Hüser,Juliane Poelchen,Huizi Wu,Karol Granados,Aniello Federico,Ke Liu,Tamara Steinfass,Marlene Vierthaler,Viktor Umansky,Jochen Utikal

Journal of cellular physiology 234:11315-11329 PubMed30565689

2018

Endoplasmic reticulum stress and unfolded protein response profile in quadriceps of sarcopenic patients with respiratory diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Barreiro,Anna Salazar-Degracia,Antonio Sancho-Muñoz,Joaquim Gea

View all publications

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