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Mouse Monoclonal EIF2A antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat, African green monkey samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF2A aa 400 to C-terminus.

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Images

Western blot - Anti-eIF2A antibody [3A7B11] (AB181467), expandable thumbnail
  • Western blot - Anti-eIF2A antibody [3A7B11] (AB181467), expandable thumbnail
  • Western blot - Anti-eIF2A antibody [3A7B11] (AB181467), expandable thumbnail
  • Flow Cytometry - Anti-eIF2A antibody [3A7B11] (AB181467), expandable thumbnail
  • Western blot - Anti-eIF2A antibody [3A7B11] (AB181467), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human EIF2A aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BY44

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
African green monkey
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/400.00000
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
1/200.00000 - 1/400.00000
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Species
Rat
Dilution info
1/200.00000 - 1/400.00000
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Species
African green monkey
Dilution info
1/200.00000 - 1/400.00000
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
Mouse
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
Rat
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
African green monkey
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Species
Rat
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Species
African green monkey
Dilution info
1/200 - 1/1000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.

Alternative names

CDA02, MSTP004, MSTP089, EIF2A, Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A

Recommended products

Mouse Monoclonal EIF2A antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat, African green monkey samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF2A aa 400 to C-terminus.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human EIF2A aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BY44
Clone number
3A7B11
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The eIF2A also referred to as eukaryotic translation initiation factor 2A is a protein involved in the initiation of mRNA translation. The eIF2A protein is distinct from eIF2α despite the similarity in their names. Its molecular weight is approximately 65 kDa. Expression of eIF2A occurs mainly in the cytoplasm of cells where it participates in the protein synthesis process. eIF2A functions as a mediator that targets the initiator methionylated-tRNA to the small ribosomal subunit in a manner independent from eIF2 complex.

Biological function summary

EIF2A impacts the translation mechanism by facilitating the binding of Met-tRNAi to the ribosome under particular conditions especially during stress responses where canonical eIF2 might be inhibited. It operates independently from the larger eIF2 complex which also includes eIF2β and eIF2γ subunits. This independent activity allows eIF2A to serve as a backup mechanism for translation initiation when the normal pathway is impaired.

Pathways

Translation regulation involves interaction with cellular stress pathways and the integrated stress response (ISR). eIF2A operates within pathways that respond to physiological stresses such as nutrient deprivation and oxidative stress which trigger alterations in protein synthesis rates. eIF2A is related to the eIF2 kinase which phosphorylates the α subunit of eIF2 leading to a general reduction in translation initiation under stress allowing selective translation of stress-response proteins.

Associated diseases and disorders

Irregularities in eIF2A function link to neurodegenerative diseases such as Alzheimer's disease and metabolic disorders like diabetes. These conditions often involve disruptions in the stress response pathways where eIF2A participates. The protein participates with others like PERK a kinase that phosphorylates eIF2α under endoplasmic reticulum stress contributing to disease-related changes in protein synthesis. By modulating translation under stress eIF2A becomes significant in these pathological contexts.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Western blot - Anti-eIF2A antibody [3A7B11] (ab181467), expandable thumbnail

    Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: eIF2A knockout HAP1 cell lysate (20 μg)
    Lane 3: Ramos cell lysate (20 μg)
    Lane 4: MOLT4 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181467 observed at 65 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
    ab181467 was shown to recognize eIF2A when eIF2A knockout samples were used, along with additional cross-reactive bands. Wild-type and eIF2A knockout samples were subjected to SDS-PAGE. ab181467 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)

    Predicted band size: 65 kDa

  • Western blot - Anti-eIF2A antibody [3A7B11] (ab181467), expandable thumbnail

    Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)

    All lanes: Western blot - Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

    Lane 1: MCF7 cell lysate

    Lane 2: PC-12 cell lysate

    Lane 3: HepG2 cell lysate

    Lane 4: HeLa cell lysate

    Lane 5: COS-7 cell lysate

    Lane 6: K562 cell lysate

    Lane 7: Jurkat cell lysate

    Lane 8: A431 cell lysate

    Lane 9: NIH3T3 cell lysate

    Predicted band size: 65 kDa

  • Western blot - Anti-eIF2A antibody [3A7B11] (ab181467), expandable thumbnail

    Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)

    Expected MWt is 40.3 kDa.

    All lanes: Western blot - Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

    All lanes: Recombinant Human eIF2A (aa 448-576) protein

    Predicted band size: 65 kDa

  • Flow Cytometry - Anti-eIF2A antibody [3A7B11] (ab181467), expandable thumbnail

    Flow Cytometry - Anti-eIF2A antibody [3A7B11] (ab181467)

    Flow cytometric analysis of HepG2 cells labeling eIF2A with ab181467 at 1/200 dilution (green) compared to a negative control (red).

  • Western blot - Anti-eIF2A antibody [3A7B11] (ab181467), expandable thumbnail

    Western blot - Anti-eIF2A antibody [3A7B11] (ab181467)

    All lanes: Western blot - Anti-eIF2A antibody [3A7B11] (ab181467) at 1/500 dilution

    Lane 1: Non-transfected HEK293 cell lysate

    Lane 2: eIF2A (aa 448-576)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 65 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody [3A7B11] (ab181467)

    Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling eIF2A with ab181467 at 1/200 dilution with DAB staining.

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Product protocols

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