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AB235147

Anti-eIF2A antibody

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(2 Publications)

Rabbit Polyclonal EIF2A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CDA02, MSTP004, MSTP089, EIF2A, Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody (AB235147)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody (AB235147)

4% paraformaldehyde-fixed, 0.3%Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling elF2A using ab235147 at 5 μg/mL overnight at 4°C in ICC/IF analysis. Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5 μg/mL for 1 hour at room temperature.

Western blot - Anti-eIF2A antibody (AB235147)
  • WB

Supplier Data

Western blot - Anti-eIF2A antibody (AB235147)

All lanes:

Western blot - Anti-eIF2A antibody (ab235147) at 5 µg/mL

Lane 1:

Daudi whole cell lysate at 35 µg

Lane 2:

MCF7 whole cell lysate at 35 µg

Lane 3:

HeLa whole cell lysate

Secondary

All lanes:

Donkey anti-Goat HRP at 1/40000 dilution

Predicted band size: 65 kDa

false

Exposure time: 35s

Western blot - Anti-eIF2A antibody (AB235147)
  • WB

Supplier Data

Western blot - Anti-eIF2A antibody (AB235147)

All lanes:

Western blot - Anti-eIF2A antibody (ab235147) at 1/1000 dilution

Lane 1:

eIF2A human protein over HEK293 expressing lysate

Lane 2:

HEK293 lysate (empty vector)

Secondary

All lanes:

Donkey Fab2 anti-Goat HRP at 1/70000 dilution

Predicted band size: 65 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

AB229912

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eIF2A also referred to as eukaryotic translation initiation factor 2A is a protein involved in the initiation of mRNA translation. The eIF2A protein is distinct from eIF2α despite the similarity in their names. Its molecular weight is approximately 65 kDa. Expression of eIF2A occurs mainly in the cytoplasm of cells where it participates in the protein synthesis process. eIF2A functions as a mediator that targets the initiator methionylated-tRNA to the small ribosomal subunit in a manner independent from eIF2 complex.
Biological function summary

EIF2A impacts the translation mechanism by facilitating the binding of Met-tRNAi to the ribosome under particular conditions especially during stress responses where canonical eIF2 might be inhibited. It operates independently from the larger eIF2 complex which also includes eIF2β and eIF2γ subunits. This independent activity allows eIF2A to serve as a backup mechanism for translation initiation when the normal pathway is impaired.

Pathways

Translation regulation involves interaction with cellular stress pathways and the integrated stress response (ISR). eIF2A operates within pathways that respond to physiological stresses such as nutrient deprivation and oxidative stress which trigger alterations in protein synthesis rates. eIF2A is related to the eIF2 kinase which phosphorylates the α subunit of eIF2 leading to a general reduction in translation initiation under stress allowing selective translation of stress-response proteins.

Irregularities in eIF2A function link to neurodegenerative diseases such as Alzheimer's disease and metabolic disorders like diabetes. These conditions often involve disruptions in the stress response pathways where eIF2A participates. The protein participates with others like PERK a kinase that phosphorylates eIF2α under endoplasmic reticulum stress contributing to disease-related changes in protein synthesis. By modulating translation under stress eIF2A becomes significant in these pathological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.
See full target information EIF2A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1249 PubMed40830221

2025

A robust method for assessing mitochondrial function in healthy and diseased frozen cardiac tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Liangyu Hu,Alexia van Rinsum,Rocco Caliandro,Xi Qi,Shuxiu Fan,Xinyi Jiang,Jur Massop,Melissa Bekkenkamp-Grovenstein,Christiane Ott,Tilman Grune,Melissa A E van de Wal,Werner J H Koopman,Marcel Giesbers,Monika Gladka,Jaap Keijer,Deli Zhang

International journal of molecular sciences 23: PubMed36430538

2022

GRP94 Inhabits the Immortalized Porcine Hepatic Stellate Cells Apoptosis under Endoplasmic Reticulum Stress through Modulating the Expression of IGF-1 and Ubiquitin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Wang,Hairui Xin,Chuang Zhang,Xianhong Gu,Yue Hao
View all publications
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Product promise

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